The acute effects of ultraviolet radiation on the blood transcriptome are independent of plasma 25OHD3

Hernández-Ferrer, Carles; Sarria, Yaris; Harrison, Graham I.; Nonell, Lara; Kang, Wenjing; Friedländer, Marc R.; Estivill, Xavier; González, Juan R.; Nieuwenhuijsen, Mark; Young, Antony R.

doi:10.1016/j.envres.2017.07.045

Cited by 13 publications

(12 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have also reported marked transcriptional changes in human skin after a single exposure of narrow or broadband UVB . In contrast, only 21 genes were differently expressed in blood in the same volunteers after whole body exposure to 3 SED of FSSR . Although some pathways detected at 6 h and 24 h were the same, others were more prominent at a specific time point suggesting a sequential response to FSSR.…”

Section: Discussionsupporting

confidence: 80%

See 1 more Smart Citation

Dose and time effects of solar‐simulated ultraviolet radiation on the in vivo human skin transcriptome

et al. 2019

View full text Add to dashboard Cite

Summary Background Terrestrial ultraviolet (UV) radiation causes erythema, oxidative stress, DNA mutations and skin cancer. Skin can adapt to these adverse effects by DNA repair, apoptosis, keratinization and tanning. Objectives To investigate the transcriptional response to fluorescent solar‐simulated radiation (FSSR) in sun‐sensitive human skin in vivo. Methods Seven healthy male volunteers were exposed to 0, 3 and 6 standard erythemal doses (SED). Skin biopsies were taken at 6 h and 24 h after exposure. Gene and microRNA expression were quantified with next generation sequencing. A set of candidate genes was validated by quantitative polymerase chain reaction (qPCR); and wavelength dependence was examined in other volunteers through microarrays. Results The number of differentially expressed genes increased with FSSR dose and decreased between 6 and 24 h. Six hours after 6 SED, 4071 genes were differentially expressed, but only 16 genes were affected at 24 h after 3 SED. Genes for apoptosis and keratinization were prominent at 6 h, whereas inflammation and immunoregulation genes were predominant at 24 h. Validation by qPCR confirmed the altered expression of nine genes detected under all conditions; genes related to DNA repair and apoptosis; immunity and inflammation; pigmentation; and vitamin D synthesis. In general, candidate genes also responded to UVA1 (340–400 nm) and/or UVB (300 nm), but with variations in wavelength dependence and peak expression time. Only four microRNAs were differentially expressed by FSSR. Conclusions The UV radiation doses of this acute study are readily achieved daily during holidays in the sun, suggesting that the skin transcriptional profile of ‘typical’ holiday makers is markedly deregulated. What's already known about this topic? The skin's transcriptional profile underpins its adverse (i.e. inflammation) and adaptive molecular, cellular and clinical responses (i.e. tanning, hyperkeratosis) to solar ultraviolet radiation. Few studies have assessed microRNA and gene expression in vivo in humans, and there is a lack of information on dose, time and waveband effects. What does this study add? Acute doses of fluorescent solar‐simulated radiation (FSSR), of similar magnitude to those received daily in holiday situations, markedly altered the skin's transcriptional profiles. The number of differentially expressed genes was FSSR‐dose‐dependent, reached a peak at 6 h and returned to baseline at 24 h. The initial transcriptional response involved apoptosis and keratinization, followed by inflammation and immune modulation. In these conditions, microRNA expression was less affected than gene expression.

show abstract

Section: Discussionsupporting

confidence: 80%

“…The study was conducted according to the Declaration of Helsinki after approval was obtained from the Ethics Committee of St Thomas's Hospital, London, U.K. All participants gave written informed consent. Seven healthy men with similar sun‐sensitive skin types II were enrolled (Table and Fig. S1; see Supporting Information).…”

Section: Methodsmentioning

confidence: 99%

Dose and time effects of solar‐simulated ultraviolet radiation on the in vivo human skin transcriptome

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The production of UVR‐induced vitamin D metabolites also did not correlate with FSSR‐induced transcriptional changes. It had previously been reported that the concentration of 25(OH)D 3 in plasma continued to rise for 48 h after erythemal FSSR, while the transcriptional changes in blood cells were greater at 6 h and had returned to initial levels by 24 h. The same pattern of timing after FSSR exposure was exhibited in the skin as described in the present study . The second finding was reduced expression of many core pigment genes 6 h after exposure to FSSR, and thus a brake was being applied on the production of potentially toxic levels of pigment.…”

supporting

confidence: 83%

“…There were no significant changes in gene expression levels in FSSR‐unexposed control skin biopsies collected at 6 h and 24 h. These biopsies were important controls to ensure that any changes in genes following 24‐h circadian expression were not misinterpreted; such controls were not possible in the study of UVR‐induced changes to blood cell transcriptomes over 24 h …”

mentioning

confidence: 99%

The changing transcriptome in human skin following in vivo exposure to erythemal solar‐simulated ultraviolet radiation

Hart

2019

Br J Dermatol

View full text Add to dashboard Cite

show abstract

“…More recently, there has been greater awareness of the diversity of cutaneous mediators released in response to UVR, which also has a large effect on the skin transcriptome [ 5 , 6 ]. There is also evidence that solar UVR may affect the blood transcriptome [ 6 , 7 ], including genes for immunity. This suggests that reported beneficial effects of sunlight may be mediated by multiple signalizing molecules, individually or in concert, rather than via a single mediator such as vitamin D. Hence, in the following discussion, higher vitamin D concentrations (measured as serum 25-hydroxy vitamin D (25(OH)D)) are considered as a proxy for sun exposure so that their association with health benefits will be taken as indicating a beneficial effect of sunlight but not necessarily a benefit of vitamin D.…”

Section: Introductionmentioning

confidence: 99%

Insufficient Sun Exposure Has Become a Real Public Health Problem

Alfredsson

Armstrong

Butterfield

et al. 2020

IJERPH

Self Cite

108

View full text Add to dashboard Cite

This article aims to alert the medical community and public health authorities to accumulating evidence on health benefits from sun exposure, which suggests that insufficient sun exposure is a significant public health problem. Studies in the past decade indicate that insufficient sun exposure may be responsible for 340,000 deaths in the United States and 480,000 deaths in Europe per year, and an increased incidence of breast cancer, colorectal cancer, hypertension, cardiovascular disease, metabolic syndrome, multiple sclerosis, Alzheimer’s disease, autism, asthma, type 1 diabetes and myopia. Vitamin D has long been considered the principal mediator of beneficial effects of sun exposure. However, oral vitamin D supplementation has not been convincingly shown to prevent the above conditions; thus, serum 25(OH)D as an indicator of vitamin D status may be a proxy for and not a mediator of beneficial effects of sun exposure. New candidate mechanisms include the release of nitric oxide from the skin and direct effects of ultraviolet radiation (UVR) on peripheral blood cells. Collectively, this evidence indicates it would be wise for people living outside the tropics to ensure they expose their skin sufficiently to the sun. To minimize the harms of excessive sun exposure, great care must be taken to avoid sunburn, and sun exposure during high ambient UVR seasons should be obtained incrementally at not more than 5–30 min a day (depending on skin type and UV index), in season-appropriate clothing and with eyes closed or protected by sunglasses that filter UVR.

show abstract

The acute effects of ultraviolet radiation on the blood transcriptome are independent of plasma 25OHD3

Cited by 13 publications

References 69 publications

Dose and time effects of solar‐simulated ultraviolet radiation on the in vivo human skin transcriptome

Dose and time effects of solar‐simulated ultraviolet radiation on the in vivo human skin transcriptome

The changing transcriptome in human skin following in vivo exposure to erythemal solar‐simulated ultraviolet radiation

Insufficient Sun Exposure Has Become a Real Public Health Problem

Contact Info

Product

Resources

About