Sixteen male, elite-level gymnasts performed 90 s of intermittent (3 x 30 s with 30 s rest) or continuous stretching (90 s) of the quadriceps muscle. A single-leg stretching and jumping design was used, with the contra-lateral limb serving as a control. The same individuals performed both conditions with alternate legs in a randomized, counterbalanced order. One leg CMJ height was measured for the stretched and the control leg after warm-up, immediately after stretching, and at regular intervals for 10 min after stretching. Range of motion (ROM) of the hip and knee joints was measured before, after, and 10 min post-stretching. Compared to the control leg, intermittent stretching increased CMJ height by 8.1±2.0%, 4 min into recovery (+2.2±2.0 cm, 95%CI: 1.0-3.4 cm, p=0.001), while continuous stretching decreased CMJ height by 17.5±3.3% immediately after (-2.9±1.7 cm, 95%CI: -2.0 to -3.7 cm, p=0.001) and by 12.0±2.7% one min after stretching (-2.2±2.1 cm, 95%CI: -1.2 to -3.2 cm, p=0.001). The increases in hip (2.9 and 3.6, p=0.001. d=2.4) and knee joint ROM (5.1 and 6.1, p=0.001. d=0.85) after the intermittent and continuous stretching protocols were not different. The opposite effects of intermittent vs. continuous stretching on subsequent CMJ performance suggests that stretching mode is an important variable when examining the acute effects of static stretching on performance in flexibility-trained athletes.