The Actual Condition Elucidation of Microbiome in a Cool or Heat Pit Part 2: Comparison of Fungal Flora Between Indoor Environments and the Environment in a Pit

Abstract

The air and the surface fungal communities were collected from a cool/heat pit and two offices during the 2019 heating and the 2021 cooling seasons. A total of 16 samples were analyzed with ITS2. The results showed that 92.2% (heating season) and 82.3% (cooling season) of each collected fungi were classified under the phylum Basidiomycota and Ascomycota. Genera Malassezia, Cladosporium and Mycosphaerella were predominantly detected in both seasons. Schizophyllum commune and Aspergillus fumigatus were predominantly detected indoors. Fungi in the pit during the cooling season can affect offices and countermeasures are needed. This does not occur in the heating season.