The activity of immunoglobulin Y anti-Mycobacterium tuberculosis on proliferation and cytokine expression of rat peripheral blood mononuclear cells

Abstract

Objective:It has long been known that chickens, like mammals, are capable of producing antigen-specific immunoglobulin Y (IgY), which functions similar to IgG. The present study was performed to investigate the activity of IgY anti-Mycobacterium tuberculosis on proliferation, interleukin (IL)-2, and interferon (IFN)-γ expression of rat peripheral blood mononuclear cells (PBMCs).Materials and Methods:The activity of IgY anti-M. tuberculosis in different doses (25, 50, and 100 μg/ml) on rat PBMCs proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. The production of IL-2 and IFN-γ in the PBMC supernatant was determined using enzyme-linked immunosorbent assay. Investigation was performed on mRNA expression of IL-2 and IFN-γ by reverse transcription-polymerase chain reaction (RT-PCR).Results:IgY anti-M. tuberculosis significantly increased the proliferation of rat PBMC. Furthermore, IgY anti-M. tuberculosis dose dependently increased IL-2 and IFN-γ production in PBMC, suggesting that pharmacological activities of IgY anti-M. tuberculosis in PBMC may be mediated by regulating the production of cytokines. In the RT-PCR, expression of cytokines such as IL-2 and IFN-γ in PBMC cultures was increased by IgY anti-M. tuberculosis.Conclusions:We concluded that increasing IL-2 and IFN-γ productions in PBMC was related to IgY anti-M. tuberculosis, stimulating the mRNA transcription (gene expression) of these cytokines which can induce proliferation of PBMC.SUMMARY Lohman laying hens immunized intramuscularly with antigens of M. tuberculosis can produce specific IgY anti-Mycobacterium tuberculosis complexIgY anti-M. tuberculosis significantly increased the proliferation of rat peripheral blood mononuclear cell (PBMC)IgY anti-M. tuberculosis dose dependently increased interleukin 2 (IL-2) and interferon (IFN)-γ production in PBMCIn the reverse transcription-polymerase chain reaction, expression of cytokines such as IL-2 and IFN-γ in PBMC cultures was increased by IgY anti-M. tuberculosisThe increasing IL-2 and IFN-γ productions in PBMC were related to stimulation on mRNA transcription which can induce proliferation of PBMC. Abbreviations Used: IgY anti-M. tuberculosis: Immunoglobulin Y anti-Mycobacterium tuberculosis; IL-2: Interleukin-2; IFN-γ: Interferon-γ; PBMCs: Peripheral blood mononuclear cells.

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

“…IgY against Mycobacterium tuberculosis increased the proliferation of PBMC (peripheral blood mononuclear cells) in rats in a dose-dependent manner. An increased expression of cytokines that stimulate the cellular immune response was observed and an increase of mRNA level of IL-2 and IFN-γ was detected by RT-PCR, which indicates that anti-M. tuberculosis IgY is a potential immunotherapeutic that stimulates T-helper 1 immune response [66].…”

Immunoglobulin Y for Potential Diagnostic and Therapeutic Applications in Infectious Diseases

“…MBTC is increasingly becoming more difficult to treat due to antibiotic resistance ( 122 ). In a rat peripheral blood mononuclear cell model, administration of high concentrations of IgY anti-MBTC increased interleukin-2 and interferon expression ( 123 ). IgY against MBTC may warrant evaluation for use in combination with other immunotherapeutic treatments of tuberculosis ( 124 ).…”

The Research Progress in Immunotherapy of Tuberculosis

“…Similar to other immunoglobulins, IgY is a class of recognition proteins formed by the immune system in response to foreign substances. Sudjarwo et al found that IgY could significantly increase the proliferation of rat PBMCs and secretion of IL-2 and IFN-g in PBMCs, suggesting that the pharmacological activity of IgY against Mtb may be mediated by regulating the production of cytokines (Sudjarwo et al, 2017a).…”

“…Sudjarwo et al . found that IgY could significantly increase the proliferation of rat PBMCs and secretion of IL-2 and IFN-γ in PBMCs, suggesting that the pharmacological activity of IgY against Mtb may be mediated by regulating the production of cytokines ( Sudjarwo et al., 2017a ).…”