The activity of adenosine deaminase and the level of nitric oxide in spinal cord of methotrexate administered rats: Protective effect of caffeic acid phenethyl ester

Uzar, Ertuğrul; Şahin, Önder; Koyuncuoğlu, Hasan Rıfat; Uz, Efkan; Baş, Orhan; Kilbas, Serkan; Yılmaz, H. Ramazan; Yürekli, Vedat Ali; Küçüker, Hüdaverdi; Songür, Ahmet

doi:10.1016/j.tox.2005.10.014

Cited by 50 publications

(45 citation statements)

References 43 publications

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“…Similar to the hepatic damage, oxidative stress and neutrophil infiltration were found to cause the renal damage (13), and a potential radical scavenger, caffeic acid phenethyl ester, had shown a protective effect against the MTX-induced renal damage (24). In rats administered with MTX, their increased levels of MDA, myeloperoxidase, and collagen content were statistically reduced, whereas in rats administered with L-carnitine, GSH levels were increased (21).…”

Section: Discussionmentioning

confidence: 86%

Vitamin C attenuates methotrexate-induced oxidative stress in kidney and liver of rats

et al. 2017

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Like several other anticancer drugs, methotrexate (MTX) causes side effects, such as neuropathic pain, hepatotoxicity, and nephrotoxicity. Abnormal production of reactive oxygen species has been suspected in the pathophysiology of MTX-induced hepatorenal toxicity. Therefore, the aim of this study was to investigate the probable protective role of vitamin C (Vit C) on oxidative stress induced by MTX in the liver and kidney tissues of rats. A total of 32 rats were randomly and equally divided into four groups. The first group served as the control group. The second group received a single dose of 20 mg/kg of MTX intraperitoneally. To demonstrate our hypothesis, the third and the fourth groups received 250 mg/kg of Vit C for 3 days by oral gavage, with or without MTX treatment. At the end of the study, the liver and kidney tissues of the rats were collected and examined using histology. Both the tissues were assayed for malondialdehyde concentration and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities. In hepatic and renal tissues, lipid peroxidation levels were increased, whereas SOD, CAT, and GSH-Px levels were decreased by MTX. All parameters, including CAT levels in hepatic tissue, were significantly restored after the administration of Vit C for 3 days. Similar to the biochemical findings, evidence of oxidative damage was examined in both types of tissues by histopathological examination. From the results of this study, we were able to observe that Vit C administration modulates the antioxidant redox system and reduces the renal and hepatic oxidative stress induced by MTX. Vit C can ameliorate the toxic effect of MTX in liver and kidney tissues of rat.

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Section: Discussionmentioning

confidence: 86%

Vitamin C attenuates methotrexate-induced oxidative stress in kidney and liver of rats

et al. 2017

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“…[17] The protective effects of CAPE have been practiced in various studies dealing with cerebral ischemia-reperfusion injury, spinal cord ischemia-reperfusion injury, Parkinson's disease, hypoxic ischemic brain damage in newborns, multiple sclerosis, convulsions, brain tumors, toxic effects of therapeutic agent in anticancer therapies, and hepatic encephalopathy. [3][4][5][6][7][8][9][10][11] There are only five studies in the literature evaluating the effects of CAPE in the spinal cord. [5,8,11,18,19] Two of these studies have been performed to evaluate the protective effects of CAPE against experimental allergic encephalomyelitisinduced oxidative stress, and in methotrexate administered rats.…”

Section: Discussionmentioning

confidence: 99%

“…[3][4][5][6][7][8][9][10][11] There are only five studies in the literature evaluating the effects of CAPE in the spinal cord. [5,8,11,18,19] Two of these studies have been performed to evaluate the protective effects of CAPE against experimental allergic encephalomyelitisinduced oxidative stress, and in methotrexate administered rats. [8,11] Kasai et al have reported that CAPE might be a promising therapeutic agent for reducing secondary neural damage in their hemi-transection model.…”

Section: Discussionmentioning

confidence: 99%

“…[5,8,11,18,19] Two of these studies have been performed to evaluate the protective effects of CAPE against experimental allergic encephalomyelitisinduced oxidative stress, and in methotrexate administered rats. [8,11] Kasai et al have reported that CAPE might be a promising therapeutic agent for reducing secondary neural damage in their hemi-transection model. [18] In the remaining two studies, researchers have evaluated the effects of CAPE in ischemia-reperfusion injury model.…”

Section: Discussionmentioning

confidence: 99%

“…Protective effects of CAPE in the nervous system have been reported in cerebral ischemic reperfusion injury, ischemic damage of the spinal cord, Parkinson's disease, convulsions, multiple sclerosis, brain tumors, hepatic encephalopathy, and against toxic effects of therapeutic agents in anticancer therapy. [3][4][5][6][7][8][9][10][11] Moreover, studies evaluating its protective effect in spinal cord ischemia-reperfusion models and hemi-transection model have been reported; however, there is no study investigating the acute effects of CAPE in traumatic acute spinal cord injury in clip compression model. Therefore, this study was conducted to evaluate the early anti-cytotoxic effects of CAPE in acute spinal cord injury.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Effects of Caffeic Acid Phenethyl Ester on Inflammatory Cytokines after Acute Spinal Cord Injury

Gülşen

Karaaslan

et al. 2015

Ulus Travma Acil Cerrahi Derg

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Decrease of adenosine deaminase activity and increase of the lipid peroxidation after acute methotrexate treatment in young rats: protective effects of grape seed extract

Pinheiro

Pimentel

Bona

et al. 2009

Cell Biochemistry & Function

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The methotrexate (MTX) is an anti-folate used to treat cancer and some inflammatory diseases. The efficacy of MTX is often limited by its severe toxicity. The present study was undertaken to determine whether Grape seed (Cabernet Sauvignon) extract (GSE) could ameliorate the MTX-induced oxidative injury and the effect on adenosine deaminase activity (ADA) in rats. The rats were pretreated with 50 mg/kg of GSE, i.p., prior to MTX administration (10 mg/kg, i.p.) with a second dose given 4 h and a third dose 16 h after MTX administration. Biochemical parameters were investigated 48 h after the last MTX administration. The administration of MTX increased thiobarbituric acid reactive species (TBARS) levels in hippocampus, kidney and liver, whereas induced a significant decreased in the ADA activity in the cerebral cortex, kidney and liver tissues. MTX administration significantly increased the activity of ALT(alanine aminotransferase) and urea levels and decreased uric acid levels in the serum. Urinary uric acid levels decreased in the MTX group when compared to those of the control group. The GSE along with MTX-administration significantly reversed these parameters toward to near normal. These results indicated that GSE could reduce hepatic and nephritic damage induced by MTX-treatment in young rats therefore having free radical scavenging.

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The activity of adenosine deaminase and the level of nitric oxide in spinal cord of methotrexate administered rats: Protective effect of caffeic acid phenethyl ester

Cited by 50 publications

References 43 publications

Vitamin C attenuates methotrexate-induced oxidative stress in kidney and liver of rats

Vitamin C attenuates methotrexate-induced oxidative stress in kidney and liver of rats

The Effects of Caffeic Acid Phenethyl Ester on Inflammatory Cytokines after Acute Spinal Cord Injury

Decrease of adenosine deaminase activity and increase of the lipid peroxidation after acute methotrexate treatment in young rats: protective effects of grape seed extract

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