The activity in the chicken alimentary tract of bacteriophages lytic for Salmonella typhimurium

Berchieri, Ângelo; Lovell, M. A.; Barrow, Paul

doi:10.1016/0923-2508(91)90187-f

Cited by 143 publications

(109 citation statements)

References 9 publications

Supporting

Mentioning

103

Contrasting

Unclassified

Order By: Relevance

“…A previous study reported similar results as the present study, where bacteriophages was reported as a factor resulting in reduced Salmonella CFU/g caecal content and reduced Salmonella colonization in broiler chicks after 5 days of treatment [26]. Bacteriophages have also been reported to reduce the viability of S. typhimurium in chicken cecum for up to 12 hours after inoculation [25]. …”

Section: Discussionsupporting

confidence: 89%

“…Spot tests were used in this study to confirm the presence of specific bacteriophages in the preparations, other studies such as Rahaman et al [17], have used similar strategies to test for the presence of specific bacteriophages. Further, the presence of bacteriophages during the experiment was verified by isolation of phages from caecal contents of chicks treated with bacteriophages, a similar method used in previous studies [25,26]. …”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Assessing the impact of bacteriophages in the treatment of Salmonella in broiler chickens

Nabil¹,

Tawakol²,

Hassan³

2018

Infection Ecology & Epidemiology

View full text Add to dashboard Cite

Salmonellosis is one of the main bacterial infections affecting commercial poultry, causing losses to poultry production, and posing a public health concern.Samples from internal organs (liver, cecum and spleen) of one hundred diseased broiler chickens were collected and subjected to Salmonella isolation, identification and serotyping. S. typhimurium and S. enteritidis were selected from the isolated Salmonella to prepare bacteriophages from sewage water taken at broiler farms. An experimental infection of one day old specific pathogen free (SPF) chicks followed by treatment with the prepared bacteriophages isolated from both Salmonella was performed. Caecal samples from infected chicks were subjected at intervals to bacteriophage isolation and Salmonella quantitation. The effectiveness of bacteriophage treatments on Salmonella colonization in cecum of infected chicks increased after five successive doses. At 3 day post infection (dpi), cecal contents showed a marginal decrease in Salmonella loads with more reduction at 5 dpi. From 7 dpi to the end of the experiment at 15 dpi, all the chicks were cleared for both Salmonella.The findings of this study demonstrate that bacteriophage treatment is efficacious in reducing S. typhimurium and S. enteritidis colonization in broiler chickens within a short period and could be used as an alternative to antibiotics.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 97%

Assessing the impact of bacteriophages in the treatment of Salmonella in broiler chickens

Nabil¹,

Tawakol²,

Hassan³

2018

Infection Ecology & Epidemiology

View full text Add to dashboard Cite

show abstract

“…Another study in which the action of the phage was only passive was reported by Berchieri et al (1991), who used Salmonella phage to treat chicks orally infected with Salmonella typhimurium. Although mortality was reduced from 60 to 3%, large numbers of phage were needed and there was no evidence of in vivo phage multiplication.…”

Section: Categories Of Behaviour Andmentioning

confidence: 99%

Understanding Bacteriophage Therapy as a Density-dependent Kinetic Process

Payne

Jansen

2001

Journal of Theoretical Biology

204

225

View full text Add to dashboard Cite

Studies of bacteriophage as therapeutic agents have had mixed and unpredictable outcomes. We argue that interpretation of these apparently paradoxical results requires appreciation of various density-dependent threshold e!ects. We use a mathematical model to delineate di!erent categories of outcome, including therapy by simple inundation, by active biocontrol, and by delayed active biocontrol. Counter-intuitively, there are situations in which earlier inoculation can be less e$cacious, and simultaneous inoculation with antibiotics can be detrimental. Predictions of therapeutic responses are made using formulae dependent on biologically meaningful parameters; experimental measurement of the parameters will be a prerequisite of application of the model to particular study systems. Such modelling can point to which aspects of phage biology might most fruitfully be engineered so as to enhance the viability of bacteriophage therapy.

show abstract

“…Animal experiments conducted with scientific rigor showed that phage therapy is effective in treating various bacterial infections (4,5,20,31), and one study (30) indicated that it might even be superior to antibiotics. Several cases in which temperate phage led to increased virulence of the respective pathogens have been reported (23,34).…”

mentioning

confidence: 99%

Therapy of Experimental Pseudomonas Infections with a Nonreplicating Genetically Modified Phage

Hagens

Habel

Ahsen

et al. 2004

Antimicrob Agents Chemother

168

107

View full text Add to dashboard Cite

Bacteriophage therapy of bacterial infections has received renewed attention owing to the increasing prevalence of antibiotic-resistant pathogens. A side effect of many antibiotics as well as of phage therapy with lytic phage is the release of cell wall components, e.g., endotoxins of gram-negative bacteria, which mediate the general pathological aspects of septicemia. Here we explored an alternative strategy by using genetically engineered nonreplicating, nonlytic phage to combat an experimental Pseudomonas aeruginosa infection. An export protein gene of the P. aeruginosa filamentous phage Pf3 was replaced with a restriction endonuclease gene. This rendered the Pf3 variant (Pf3R) nonreplicative and concomitantly prevented the release of the therapeutic agent from the target cell. The Pf3R phage efficiently killed a wild-type host in vitro, while endotoxin release was kept to a minimum. Treatment of P. aeruginosa infections of mice with Pf3R or with a replicating lytic phage resulted in comparable survival rates upon challenge with a minimal lethal dose of 3. However, the survival rate after phage therapy with Pf3R was significantly higher than that with the lytic phage upon challenge with a minimal lethal dose of 5. This higher survival rate correlated with a reduced inflammatory response elicited by Pf3R treatment relative to that with the lytic phage. Therefore, this study suggests that the increased survival rate of Pf3R-treated mice could result from reduced endotoxin release. Thus, the use of a nonreplicating modified phage for the delivery of genes encoding proteins toxic to bacterial pathogens may open up a new avenue in antimicrobial therapy.

show abstract

The activity in the chicken alimentary tract of bacteriophages lytic for Salmonella typhimurium

Cited by 143 publications

References 9 publications

Assessing the impact of bacteriophages in the treatment of Salmonella in broiler chickens

Assessing the impact of bacteriophages in the treatment of Salmonella in broiler chickens

Understanding Bacteriophage Therapy as a Density-dependent Kinetic Process

Therapy of Experimental Pseudomonas Infections with a Nonreplicating Genetically Modified Phage

Contact Info

Product

Resources

About