The active transport of 5‐hydroxyindol‐3‐ylacetic acid and 3‐methoxy‐4‐hydroxyphenylacetic acid from a recirculatory perfusion system of the cerebral ventricles of the unanaesthetized dog

Ashcroft, G. W.; Dow, R. C.; Moir, A. T. B.

doi:10.1113/jphysiol.1968.sp008660

Cited by 155 publications

(48 citation statements)

References 30 publications

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“…Animal methodology The animals used in these experiments were adult male beagles, 10-14 kg which had had guide tubes directed towards the lateral ventricles implanted in the skull (Ashcroft, Dow & Moir, 1968b) at least two weeks before any subsequent experimental procedure.…”

Section: Methodsmentioning

confidence: 99%

“…From the rate of infusion of exogenous 5-HIAA and the equilibrium concentrations of 5-HIAA in the perfusion system before and during this infusion, the rate at which exogenous 5-HIAA was removed from the c.s.f. system was calculated (Ashcroft et al, 1968b). It was assumed that this was also the rate at which endogenous 5-HIAA was removed from the c.s.f.…”

Section: Ventricular Perfusion Experimentsmentioning

confidence: 99%

“…Recirculatory perfusion of the ventricular space from lateral ventricle to cisterna magna was carried out with donor c.s.f. while the dogs were conscious and unrestrained as has been fully described previously (Ashcroft et al, 1968b). Each recirculatory perfusion experiment was carried out in two parts.…”

Section: Ventricular Perfusion Experimentsmentioning

confidence: 99%

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Interaction in the cerebral metabolism of the biogenic amines. Effects of phenelzine on the cerebral metabolism of the 5‐hydroxyindoles in dog brain

Moir

1972

British J Pharmacology

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Summary Chronic administration of phenelzine to dogs caused 5‐hydroxyindol‐3‐ylacetic acid (5‐HIAA) concentrations in c.s.f. from the lateral ventricle to be maintained at 50% of their normal values, but did not alter the concentrations of 5‐HIAA in c.s.f. from the cisterna magna. Following 10 days treatment with phenelzine, the active transport of 5‐HIAA from c.s.f. which normally occurs in the region of the fourth ventricle, was inhibited. This transport system was also inhibited by the addition of phenylacetic acid, the acid metabolite of phenelzine, to the fluid perfusing the cerebral ventricles. After 10–12 days treatment with phenelzine all regions of brain showed concentration increases to approximately 300% for 5‐HT and 150% for 5‐HIAA, but no alteration in the tryptophan concentration. Intravenous administration of tryptophan to dogs pretreated with phenelzine caused large increases in the concentration of tryptophan in brain and body fluids but did not alter either the concentrations of 5‐hydroxytryptamine and 5‐HIAA in brain or of 5‐HIAA in c.s.f. A model of the cerebral metabolism of 5‐hydroxytryptamine is proposed and the results are interpreted to mean that phenelzine has inhibitory actions, either directly or in some instances indirectly, on intracerebral tryptophan 5‐hydroxylase, monoamine oxidase and the transport of 5‐HIAA from both brain and c.s.f.

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Section: Methodsmentioning

confidence: 99%

Section: Ventricular Perfusion Experimentsmentioning

confidence: 99%

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Interaction in the cerebral metabolism of the biogenic amines. Effects of phenelzine on the cerebral metabolism of the 5‐hydroxyindoles in dog brain

Moir

1972

British J Pharmacology

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“…Four male Beagle dogs with indwelling ventricular cannulae (Ashcroft, Dow & Moir, 1968) had 0.5 ml of CSF withdrawn on 3 occasions, at 0, 30 and 60 min before an intravenous loading dose of equal volumes of 0.9% w/v NaCl solution (saline), or D or Ltryptophan (50mg/kg made up in saline at a concentration of 20 mg/ml). This dose was chosen as that producing a change in 5-HT synthesis when cisternal CSF was used as an index of cerebral turnover (Eccleston, Ashcroft, Moir, Parker-Rhodes, Lutz & O'Mahoney, 1968).…”

Section: Methodsmentioning

confidence: 99%

A COMPARISON OF d AND l‐TRYPTOPHAN ON THE CEREBRAL METABOLISM OF 5‐HYDROXYTRYPTAMINE AND DOPAMINE IN THE DOG

Budny¹,

Dow

Eccleston

et al. 1976

British J Pharmacology

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After d and l‐tryptophan (50 mg/kg) were given intravenously in the dog, the concentration of the amino acid was increased in ventricular cerebrospinal fluid (CSF) during the subsequent 4 h of sampling, although the concentrations were significantly lower following the administration of the d‐isomer. There was no evidence that d‐tryptophan increased the synthesis of 5‐hydroxytryptamine (5‐HT) in dog brain as judged by the failure to cause a change in 5‐hydroxyindoleacetic acid (5‐HIAA) concentrations in ventricular CSF different from that seen with controls. There was no appreciable conversion of d‐tryptophan to l‐tryptophan in the dog. d‐tryptophan was cleared more rapidly from plasma than l‐tryptophan. No difference in plasma binding between d and l‐tryptophan was detected.

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“…It is localized in the region of the fourth ventricle (8). In CSF, there is a gradient in the homovanillic acid concentration: in man, the concentration falls from 466 £/1 in the ventricular CSF to 185 in the cisternal and to 53 /ig/1 in the lumbar CSF (9).…”

Section: Introductionmentioning

confidence: 99%

Gas Chromatographic Estimation of Homovanillic Acid in Serum of Normals and Psychotic Patients

Markianos¹,

Rüther²

1976

Clinical Chemistry and Laboratory Medicine

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Summary: Homovanillic acid is extracted from 0.5 ml serum with ethyl acetate at acidic pH, and its pentafluoropropionic anhydride and hexafluoroisopropanol derivative injected into a 3% SE-30 column at 140 °C, fitted with an electron capture detector. In a group of normal volunteers (n = 42) a mean value of 62 ± 45 jug/1 was found. The distribution of the serum concentrations was found to be bimodal. Using the same procedure for the homovanillic acid estimation in cerebrospinal fluid (CSF), mean values of 73.0 ± 41 ßg/i serum and 68 ± 36 %/\ CSF were found for a group of 22 untreated patients with paranoid-hallucinatory syndrome. After treatment with neuroleptics, for 1 week to 1 month, the homovanillic acid concentration increased significantly only in the CSF. By the procedure described, other acidic metabolites of biogenic amines are extracted together with homovanillic acid and can also be estimated in the same sample. Gaschromatographische Bestimmung von Homovanillinsäure im Serum Gesunder und psychotischer PatientenZusammenfassung: Homovanülinsäure wird aus 0.5 ml Serum mit Ethylacetat bei saurem pH extrahiert und nach Derivatisierung mit Pentafluorpropionsäureanhydrid und Hexafluorisopropanol auf einer 3% SE-30 Säule gaschromatographisch getrennt und mit Elektroneneinfangdetektor bestimmt. Für eine Gruppe von Normalpersonen (n = 42) wurde ein Mittelwert von 62 ± 45 #g/l Serum ermittelt, und die Verteilung der Konzentrationen war bimodal. Die gleiche Methode kann für die Homo vanillinsäure Bestimmung im Liquor cerebrospinalis angewandt werden. In einer Gruppe psychotischer Patienten mit paranoid-halluzinatorischem Syndrom (n = 22), unbehandelt, wurden Mittelwerte von 73 ± 41 /zg/1 Serum und 68 ± 36 g/l Liquor gefunden. Nach Behandlung mit Neuroleptika kam es zu einer Homovanillinsäure Anstieg nur im Liquor, Die Methode erfaßt außer Homovanülinsäure auch andere saure Metabolite biogener Amine.

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The active transport of 5‐hydroxyindol‐3‐ylacetic acid and 3‐methoxy‐4‐hydroxyphenylacetic acid from a recirculatory perfusion system of the cerebral ventricles of the unanaesthetized dog

Cited by 155 publications

References 30 publications

Interaction in the cerebral metabolism of the biogenic amines. Effects of phenelzine on the cerebral metabolism of the 5‐hydroxyindoles in dog brain

Interaction in the cerebral metabolism of the biogenic amines. Effects of phenelzine on the cerebral metabolism of the 5‐hydroxyindoles in dog brain

A COMPARISON OF d AND l‐TRYPTOPHAN ON THE CEREBRAL METABOLISM OF 5‐HYDROXYTRYPTAMINE AND DOPAMINE IN THE DOG

Gas Chromatographic Estimation of Homovanillic Acid in Serum of Normals and Psychotic Patients

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