1983
DOI: 10.1016/0161-5890(83)90008-1
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The active site of human C4a anaphylatoxin

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Cited by 21 publications
(8 citation statements)
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“…Variations between the C-terminal pentapeptide AGLQR in C4a and the corresponding peptide LGLAR in C3a are particularly interesting changes because they result in functional consequences. Substitution of a glutamine in human C4a corresponding to the alanine at position 76 of C3a is largely responsible for the lower bioactivity of C4a relative to C3a as deduced from synthetic peptide studies [50].…”
Section: A Primary Structure Of C3a C4a and C5amentioning
confidence: 99%
See 1 more Smart Citation
“…Variations between the C-terminal pentapeptide AGLQR in C4a and the corresponding peptide LGLAR in C3a are particularly interesting changes because they result in functional consequences. Substitution of a glutamine in human C4a corresponding to the alanine at position 76 of C3a is largely responsible for the lower bioactivity of C4a relative to C3a as deduced from synthetic peptide studies [50].…”
Section: A Primary Structure Of C3a C4a and C5amentioning
confidence: 99%
“…A series of synthetic C4a peptides were prepared and characterized in the same manner as those of the C3a series [50]. Although these synthetic model peptides are considerably less active than the corresponding C3a peptides, the C-terminal pentaand octapeptides of C4a did exhibit measurable activity indicating that the "active The upper view projects a predominantly hydrophobic surface while the lower view shows the arginine cluster and a predominantly hydrophilic surface.…”
Section: C3a Analoguesmentioning
confidence: 99%
“…Functional- [5] and immunoblot assays [6,7] of plasma from HAE patients show degradation of high molecular weight kininogen (HK) into smaller fragments, compatible with liberation of bradykinin, the major mediator of edema formation. A kinin-like peptide cleaved off from C2b by plasmin [8], and C4a [9] are probably less important mediators. Fibrinolysis may be enhanced as shown by elevated plasmin-alpha-2-antiplasmin (PAP) complexes [10,11].…”
Section: Introductionmentioning
confidence: 99%
“…Amino acid substitutions were made systematically at positions 73-76 in order to elucidate the functionally important features of their side chains. Analogues 5, 7,18, 20, and 21 have been described previously (Caporale et al, 1980;Hugli et al, 1983).…”
Section: Resultsmentioning
confidence: 99%
“…Human C4a binds to the same cellular receptor as C3a but exhibits only 1% of the spasmogenic activity of C3a (Gorski et al, 1979). This low level of activity is due to replacement of Leu-73 by alanine and Ala-76 by glutamine (Hugli et al, 1983). We expect that bovine C4a should be significantly more active than human C4a because its active-site pentapeptide is the Val-73 analogue (5), which is essentially as active as C3a active-site peptide 1 in the ileal contraction assay.…”
Section: Discussionmentioning
confidence: 94%