The active Fe chelator proline‐2′‐deoxymugineic acid enhances peanut yield by improving soil Fe availability and plant Fe status

Abstract

Iron (Fe) deficiency restricts crop yields in calcareous soil. Thus, a novel Fe chelator, proline-2′-deoxymugineic acid (PDMA), based on the natural phytosiderophore 2′-deoxymugineic acid (DMA), was developed to solve the Fe deficiency problem.However, the effects and mechanisms of PDMA relevant to the Fe nutrition and yield of dicots grown under field conditions require further exploration. In this study, pot and field experiments with calcareous soil were conducted to investigate the effects of PDMA on the Fe nutrition and yield of peanuts. The results demonstrated that PDMA could dissolve insoluble Fe in the rhizosphere and up-regulate the expression of the yellow stripe-like family gene AhYSL1 to improve the Fe nutrition of peanut plants. Moreover, the chlorosis and growth inhibition caused by Fe deficiency were significantly diminished. Notably, under field conditions, the peanut yield and kernel micronutrient contents were promoted by PDMA application. Our results indicate that PDMA promotes the dissolution of insoluble Fe and a rich supply of Fe in the rhizosphere, increasing yields through integrated improvements in soil-plant Fe nutrition at the molecular and ecological levels. In conclusion, the efficacy of PDMA for improving the Fe nutrition and yield of peanut indicates its outstanding potential for agricultural applications.

Effects of stereoisomers of proline-deoxymugineic acid on iron uptake in Poaceae plants

“…1). 15 In addition, PDMA was also shown to be effective for cucumbers and peanuts in calcareous soil, [16][17] suggesting that PDMA is applicable to all plant species, not just the Poaceae. Most recently, the structure of HvYS1, the YS1/YSL transporter of barley, was elucidated by cryo-electron microscopy, and structural analysis revealed that the PDMA-Fe(III) complex passes the same binding site as DMA-Fe(III), meaning that PDMA functions as a fertilizer in exactly the same manner as natural DMA.…”

Integrated physiological, transcriptomic and metabolomic analyses reveal the mechanism of peanut kernel weight reduction under waterlogging stress

“…It is unclear whether WS affects hormones related to the regulation of starch and sucrose metabolism and kernel weight in peanuts. Transcriptomic and metabolomic analyses have been widely used to investigate the dynamic expression of genes related to starch and sucrose metabolism (Shen et al, 2022; Wang et al, 2023; Yang et al, 2022; Zhang et al, 2015), fatty acid metabolism (Xu et al, 2022), amino acid synthesis (Lou et al, 2022), and flavour formation (Wang et al, 2021) during kernel filling. Therefore, the waterlogging‐sensitive peanut variety, Huayu 39, was selected to examine the key developmental stages during which WS affects kernel filling.…”