The active constituents of asphodelus microcarpus Salzm et Vivi

Ghaleb, H. A.; Rizk, Sameh A.; Hammouda, F. M.; Abd-Elgawad, Mahfouz M. M.

doi:10.1007/bf01105520

Cited by 7 publications

(3 citation statements)

References 2 publications

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“…A broad range of in vitro and in vivo biological activities of Asphodelus species root extracts have been documented [4] and found to have antimicrobial [8][9][10][11][12][13][14], antiparasitic [15], antimalarial [16], antitumoral [17][18][19], antioxidant [17,[20][21][22][23], anti-inflammatory [24][25][26][27], hypotensive and diuretic [28] activities. They were mainly reported to have anthraquinone derivatives, triterpenoids, and naphthalene derivatives as major secondary metabolites [13,15,16,[29][30][31][32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

Monographic Quality Parameters and Genotoxicity Assessment of Asphodelus bento-rainhae and Asphodelus macrocarpus Root Tubers as Herbal Medicines

Malmir

Serrano

Lima

et al. 2022

Plants

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Root tubers of Asphodelus bento-rainhae subsp. bento-rainhae (AbR), an endemic species with relevant interest due to conservation concerns, and Asphodelus macrocarpus subsp. macrocarpus (AmR) have been traditionally used for culinary and medicinal purposes, mainly associated with skin infection and inflammation. The present study aims to establish the quality control criteria for the proper characterization of dried root tubers of both species as herbal substances, together with their preclinical safety assessments. Botanical identification using macroscopic and microscopic techniques and phytochemical evaluation/quantification of the main classes of marker secondary metabolites, including phenolic compounds (flavonoid, anthraquinone, condensed and hydrolysable tannin) and terpenoids were performed. Additionally, in vitro genotoxicity/mutagenicity was evaluated by Ames test. Evident morphological differences in the development of tubercles (3.5 × 1 cm in AbR and 8.7 × 1.4 cm in AmR) and microscopicly in the arrangements and characteristics of the vascular cylinder (metaxylem and protoxylems) were found. Anatomical similarities such as multiple-layered epidermis (velamen) and the cortex area with thin-walled idioblasts (134 ± 2.9 µm and 150 ± 27.6 µm) containing raphide crystals (37.2 ± 14.2 µm and 87.7 ± 15.3 µm) were observed between AbR and AmR, respectively. Terpenoids (173.88 ± 29.82 and 180.55 ± 10.57 mg OAE/g dried weight) and condensed tannins (128.64 ± 14.05 and 108.35 ± 20.37 mg CAE/g dried weight) were found to be the main class of marker secondary metabolites of AbR and AmR extracts, respectively. No genotoxicity (up to 5 mg/plate, without metabolic activation) was detected in these medicinal plants’ tested extracts. The obtained results will contribute to the knowledge of the value of the Portuguese flora and their future commercial cultivation utilization as raw materials for industrial and pharmaceutical use.

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Section: Introductionmentioning

confidence: 99%

Monographic Quality Parameters and Genotoxicity Assessment of Asphodelus bento-rainhae and Asphodelus macrocarpus Root Tubers as Herbal Medicines

Malmir

Serrano

Lima

et al. 2022

Plants

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“…For example, Merendera montana contains several tropolonic alkaloids (Gómez‐García et al, 2003, 2004) that can be lethal to cattle (Pijewska et al, 1967). Similarly, tubers of Asphodelus aestivus Brot., a close relative of Asphodelus albus , are rich in alkaloids and anthraquinones (Ghaleb et al, 1972) and several alkaloids have been identified in the bulbs of Narcissus species (Bastida et al, 2006). Such chemical compounds make consumption of these geophytes by wild boar unlikely.…”

Section: Discussionmentioning

confidence: 99%

Wild‐boar disturbance increases nutrient and C stores of geophytes in subalpine grasslands

Palacio

Bueno

Azorín

et al. 2013

American J of Botany

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“…Root tubers of Asphodelus species have been traditionally used for the treatment of skin-related disorders and infections such as wounds, eczema, alopecia and psoriasis [6]. Furthermore, ethnomedical information on several Asphodelus species, supported by in vitro and in vivo biological activity studies, indicates their strong antimicrobial potential [7][8][9][10][11][12][13], particularly against resistant pathogens, due to the presence of secondary metabolites such as anthraquinones, arylcoumarins, terpenoids and naphthalene derivatives [12,[14][15][16][17][18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Bioguided Identification of Active Antimicrobial Compounds from Asphodelus bento-rainhae and Asphodelus macrocarpus Root Tubers

et al. 2023

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Root tubers of Asphodelus bento-rainhae subsp. bento-rainhae (AbR), a vulnerable endemic species, and Asphodelus macrocarpus subsp. macrocarpus (AmR) have traditionally been used in Portugal to treat inflammatory and infectious skin disorders. The present study aims to evaluate the in vitro antimicrobial activity of crude 70% and 96% hydroethanolic extracts of both medicinal plants, specifically against multidrug-resistant skin-related pathogens, to identify the involved marker secondary metabolites and also to assess the pre-clinical toxicity of these medicinal plant extracts. Bioguided fractionation of the 70% hydroethanolic extracts of both species using solvents of increasing polarity, namely diethyl ether (DEE: AbR-1, AmR-1), ethyl acetate (AbR-2, AmR-2) and aqueous (AbR-3, AmR-3) fractions, enabled the identification of the DEE fractions as the most active against all the tested Gram-positive microorganisms (MIC: 16 to 1000 µg/mL). Furthermore, phytochemical analyses using TLC and LC-UV/DAD-ESI/MS techniques revealed the presence of anthracene derivatives as the main constituents of DEE fractions, and five known compounds, namely 7′-(chrysophanol-4-yl)-chrysophanol-10’-C-beta-D-xylopyranosyl-anthrone (p), 10,7′-bichrysophanol (q), chrysophanol (r), 10-(chrysophanol-7′-yl)-10-hydroxychrysophanol-9-anthrone (s) and asphodelin (t), were identified as the main marker compounds. All these compounds showed high antimicrobial activity, particularly against Staphylococcus epidermidis (MIC: 3.2 to 100 µg/mL). Importantly, no cytotoxicity against HepG2 and HaCaT cells (up to 125 µg/mL) for crude extracts of both species and genotoxicity (up to 5000 µg/mL, with and without metabolic activation) for AbR 96% hydroethanolic extract was detected using the MTT and Ames tests, respectively. Overall, the obtained results contribute to the concrete validation of the use of these medicinal plants as potential sources of antimicrobial agents in the treatment of skin diseases.

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The active constituents of asphodelus microcarpus Salzm et Vivi

Cited by 7 publications

References 2 publications

Monographic Quality Parameters and Genotoxicity Assessment of Asphodelus bento-rainhae and Asphodelus macrocarpus Root Tubers as Herbal Medicines

Monographic Quality Parameters and Genotoxicity Assessment of Asphodelus bento-rainhae and Asphodelus macrocarpus Root Tubers as Herbal Medicines

Wild‐boar disturbance increases nutrient and C stores of geophytes in subalpine grasslands

Bioguided Identification of Active Antimicrobial Compounds from Asphodelus bento-rainhae and Asphodelus macrocarpus Root Tubers

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