The activation of the complement system by polymer materials and their blood compatibility

Севастьянов, В. И.; Tseytlina, E. A.

doi:10.1002/jbm.820180902

Cited by 50 publications

(22 citation statements)

References 12 publications

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“…Moreover, these agents tend to aggregate with serum proteins in vivo and often mediate robust complement activation with potential deleterious effects (34)(35)(36). We recently reported the development of an amphipathic peptide-based siRNA nanoplatform (13,14) that uses a modified version of the bee venom component melittin to transport siRNA (15).…”

Section: Discussionmentioning

confidence: 99%

Peptide-siRNA nanocomplexes targeting NF-κB subunit p65 suppress nascent experimental arthritis

Zhou¹,

Yan²,

Pan³

et al. 2014

J. Clin. Invest.

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Section: Discussionmentioning

confidence: 99%

Peptide-siRNA nanocomplexes targeting NF-κB subunit p65 suppress nascent experimental arthritis

Zhou¹,

Yan²,

Pan³

et al. 2014

J. Clin. Invest.

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“…A method of measuring total hemolytic activity of human serum complement before and after its incubation with samples was chosen to elucidate the relationship between the polymer surface type and complement activation [18,19]. The registered parameter is the time for 50% lysis (¿ 1=2 ) of 5 ml sensitized sheep erythrocytes (5 £ 10 8 /ml) by 100 ¹l studied serum [17].…”

Section: Investigation Of Hemocompatibility Of Phasmentioning

confidence: 99%

“…An indicator of hemocompatibility of biomaterials is the state of the hemostasis system [15,16]. It can be evaluated by parameters such as the morphology of adhering platelets [15], complement activation [17][18][19] and others.…”

Section: Introductionmentioning

confidence: 99%

Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood

Sevastianov¹,

Perova²,

Shishatskaya³

et al. 2003

Journal of Biomaterials Science, Polymer Edition

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Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only beta-OH-C14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.

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“…4,5 The concentration of C3a/C3a desArg was determined using a commercial radioimmunoassay kit (Amersham).…”

Section: Complement Activationmentioning

confidence: 99%

New anionic polyelectrolyte hydrogel for corneal surgery

Honiger

Couturier

Goldschmidt

et al. 1997

J. Biomed. Mater. Res.

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A new high-water-content (78%) anionic polyelectrolyte hydrogel was obtained by phase inversion (demixion) of a polymer solution containing 9.0% poly(acrylonitrile sodium methallylsulphonate), 85.0% dimethylformamide, and 6.0% saline solution (0.9% NaCl). The hydrogel is permeable to water, saline, urea, creatinine, glucose, human albumin, and saline-dissolved oxygen. Investigation of the interactions between human serum and surfaces prepared with the new yielded hydrogel, compared to serum interaction with silica-free silicone (RTV), regenerated cellulose (Cuprophan), MMA/PVP copolymer (Lidofilcon), PMMA (Perspex), FIFE (Gore-Tex), and poly(acrylonitrile sodium methallylsulphonate) hemodialysis membrane (AN-69), showed the hydrogel and hemodialysis membrane (both prepared with AN-69 copolymer) to be the only materials devoid of complement (C')-activating ability.

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The activation of the complement system by polymer materials and their blood compatibility

Cited by 50 publications

References 12 publications

Peptide-siRNA nanocomplexes targeting NF-κB subunit p65 suppress nascent experimental arthritis

Peptide-siRNA nanocomplexes targeting NF-κB subunit p65 suppress nascent experimental arthritis

Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood

New anionic polyelectrolyte hydrogel for corneal surgery

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