The activation of directional stem cell motility by green light-emitting diode irradiation

Ong, Wei Kee; Chen, How Foo; Tsai, Cheng Ting; Fu, Yao‐Shi; Wong, Yi Shan; Yen, Ta‐Jen; Chang, Tzu Hao; Huang, Hsien Da; Lee, Oscar K.; Chien, Shu; Ho, Jennifer H.

doi:10.1016/j.biomaterials.2012.11.065

Cited by 43 publications

(25 citation statements)

References 54 publications

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“…De Villiers et al [21] Human adipose tissue 24, 48, and 72 h after irradiation The irradiated cell group had a statistically significant increase on cell proliferation at 24 and 72 h when compared to the nonirradiated group Horvat-Karajz et al [22] Murine bone marrow 24, 48, and 72 h after the first irradiation Biostimulation at lower doses in all time point evaluated and inhibition in cell growth after 48 h at higher doses (11.7 J/cm 2 ) Mvula et al [23] Human adipose tissue 24 and 48 h after irradiation Increase of cell proliferation in irradiated cells when compared to nonirradiated cells at the intervals of 24 and 48 h Ong et al [24] Human orbital fat tissues 24, 48, and 72 h after irradiation LED irradiation showed no effect on human orbital fat stem cells proliferation Soares et al [25] Human periodontal ligament 0, 24, 48, and 72 h after irradiation The dose of 1.0 J/cm 2 promoted a significant increase in cell proliferation at 48 and 72 h when compared to the control and the group that received a dose of 0.5 J/cm 2 Giannelli et al [26] Murine bone marrow 0, 24, 48, and 72 h after irradiation Cell proliferation was significantly higher at 72 h after irradiation compared to the control group Pereira et al [27] Healthy and inflamed human dental pulp 24, 48, 72, and 96 h after irradiation…”

Section: Discussionmentioning

confidence: 98%

“…Twelve studies evaluated the effect of LLLT on proliferation of stem cells from bone marrow [1-3, 9, 10, 12, 13, 22, 26, 28-30], while the adipose tissue was the source of stem cells in four studies [8,21,23,24], dental pulp in two studies [7,27], and the periodontal ligament [25] in one paper. Table 3 shows in summary form the cell types evaluated in each paper, as well as the time points used for assessment of cell proliferation and the main findings of the studies.…”

Section: Description Of the Studiesmentioning

confidence: 99%

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Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review

et al. 2015

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Low-level laser therapy (LLLT) has been used in several in vitro experiments in order to stimulate cell proliferation. Cells such as fibroblasts, keratinocytes, lymphocytes, and osteoblasts have shown increased proliferation when submitted to laser irradiation, although little is known about the effects of LLLT on stem cells. This study aims to assess, through a systematic literature review, the effects of LLLT on the in vitro proliferation of mesenchymal stem cells. Using six different terms, we conducted an electronic search in PubMed/Medline database for articles published in the last twelve years. From 463 references obtained, only 19 papers met the search criteria and were included in this review. The analysis of the papers showed a concentration of experiments using LLLT on stem cells derived from bone marrow, dental pulp, periodontal ligament, and adipose tissue. Several protocols were used to irradiate the cells, with variations on wavelength, power density, radiation time, and state of light polarization. Most studies demonstrated an increase in the proliferation rate of the irradiated cells. It can be concluded that the laser therapy positively influences the in vitro proliferation of stem cells studied, being necessary to carry out further experiments on other cell types and to uniform the methodological designs.

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Section: Discussionmentioning

confidence: 98%

Section: Description Of the Studiesmentioning

confidence: 99%

Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review

et al. 2015

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“…de Sousa et al 36 also demonstrated that the use of green and red LED light promoted proliferation of rat fibroblasts. But Ong et al 37 reported that 530 nm LED irradiation showed no effect on human orbital fat stem cells proliferation. It is not easy to compare the experimental results of LLLI due to the variation of experimental design.…”

Section: Discussionmentioning

confidence: 99%

Biological effects of low-level laser irradiation on umbilical cord mesenchymal stem cells

Chen

Wang

et al. 2016

AIP Advances

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Low-level laser irradiation (LLLI) can enhance stem cell (SC) activity by increasing migration and proliferation. This study investigated the effects of LLLI on proliferation, enzymatic activity, and growth factor production in human umbilical cord mesenchymal SCs (hUC-MSCs) as well as the underlying mechanisms. hUC-MSCs were assigned to a control group (non-irradiation group) and three LLLI treatment groups (635 nm group, 808 nm group, and 635/808 nm group). Laser power density and energy density of 20 mW/cm2 and 12 J/cm2, respectively, were used for each experiment. The proliferation rate was higher in the 635 nm as compared to the other groups. LLLI at 808 nm did not induce cell proliferation. ROS levels in cells exposed to 635, 808, and 635/808 nm radiation were increased by 52.81%, 26.89%, and 21.15%, respectively, relative to the control group. CAT, tGPx, and SOD activity was increased. LLLI at 808 nm increased the levels of IL-1, IL-6, and NFκB but not VEGF. LLLI improved hUC-MSCs function and increased antioxidant activity. Dual-wavelength LLLI had more potent effects on hUC-MSCs than single-wavelength treatment. LLLI has potential applications in the preconditioning of hUC-MSCs in vitro prior to transplantation, which could improve the regenerative capacity of cells.

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“…Alterations in signalling molecules involved in light-mediated responses can give rise to changes in gene expression as well as in the activation of transcription factors89. The highly conserved mitogen-activated protein kinase (MAPK) family of signalling molecules are involved in the response to growth-stimulatory signals as well as to adverse conditions, such as exposure to cytotoxic and genotoxic substances and radiation.…”

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confidence: 99%

Light-induced rapid Ca2+ response and MAPK phosphorylation in the cells heterologously expressing human OPN5

Sugiyama

Suzuki

Takahashi

2014

Sci Rep

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Molecular imaging is a powerful tool for investigating intracellular signalling, but it is difficult to acquire conventional fluorescence imaging from photoreceptive cells. Here we demonstrated that human opsin5 (OPN5) photoreceptor mediates light-induced Ca2+ response in human embryonic kidney (HEK293) and mouse neuroblastoma (Neuro2a) cell lines using a luminescence imaging system with a fluorescent indicator and a newly synthesized bioluminescent indicator. Weak light fluorescence and bioluminescence imaging revealed rapid and transient light-stimulated Ca2+ release from thapsigargin-sensitive Ca2+ stores, whereas long-lasting Ca2+ elevation was observed using a conventional fluorescence imaging system. Bioluminescence imaging also demonstrated that OPN5 activation in HEK293 cells induced a decrease in pertussis toxin–sensitive cAMP, confirming previous reports. In addition, ultraviolet radiation induced the phosphorylation of mitogen-activated protein kinases when OPN5 was stimulated in Neuro2a cells. These findings suggest that the combination of these imaging approaches may provide a new means to investigate the physiological characteristics of photoreceptors.

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The activation of directional stem cell motility by green light-emitting diode irradiation

Cited by 43 publications

References 54 publications

Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review

Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review

Biological effects of low-level laser irradiation on umbilical cord mesenchymal stem cells

Light-induced rapid Ca2+ response and MAPK phosphorylation in the cells heterologously expressing human OPN5

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