Abstract:The action pattern of sugarcane leaf amylase was investigated by paper chromatographic techniques. The enzyme was extracted with water from lyophilized leaf tissues, and precipitated by ammonium sulfate between 48- and 60-percent saturation. Aliquots were removed from enzyme-starch digests and inactivated in boiling water at intervals ranging from 5 minutes to 72 hours. Samples were spotted on Whatman No. 1 filter paper, irrigated with butanol-pyridine-water (6:4:3, v/v), and products identified by the silver … Show more
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