The action of vincaleukoblastine on mitosis in vitro

Cytological effects of medicinal plants in Qatar. I. Mitotic effect of water extract of Pulicaria crispa on Allium cepa.

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Several years ago, many investigators had the interest to study the cytological effects of extracts of medicinal plants, among them, Hussein and Hakeem (1961), (Podophyllin), Tarkowska (1971), (Nerium extract), Palmer et al (1960), Cardinali et al (1961 and Srivastava et al (1973), (Vincoleukoblastine).In this survey the cytological effects of water extracts from a folkmedicinal plants in Qatar were studied.Pulicaria crispa is a perennial plant and its extract is used by the inhabitants against diarrhoea and to enhance bile secretion.

Material and methodsPreparation of the extract 200gm of the dry plant of Pulicaria crispa was boiled in 1000cc distilled water under reflux condenser for 5mn. Then the extract was filtered while hot through a suction pump.

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Cytological and Autoradiographic Studies of the Effects of Vincaleukoblastine (VLB) on the Root Meristem Cells of <i>Vicia faba</i>

“…Earlier studies (Palmer et al 1960, Cardinali et al 1961, Cutts 1961 indicated that VLB interfered with cell division in animal tissues resulting in an increase in the mitotic index due to accumulation of metaphases. The present study has shown that in addition to its effect on metaphases, VLB has some effect on pro phases as well as post-metaphasic stages.…”

“…The effects of Vincaleukoblastine (VLB) have been studied on the tissue cul ture cells, ascites tumor cells and normal bone marrow cells (Palmer et al 1960, Cardinali et al 1961, Cutts 1961. In these studies, it has been shown that VLB acts like colchicine as it produces mitotic arrest resulting in accumulation of meta phases.…”

Action of vindesine sulfate on ultrastructure of metaphasic chromosomes of human lymphocytes.

“…2, metaphase en couronne. chromosomes apparents (Palmer et al 1960); la vincristine des metaphases a chro mosomes compacts et fuseaux contractes; la vindesine des fuseaux agrandis et des chromosomes disperses; De plus, la vindesine est trois fois plus efficace que la vincristine, dix fois plus que la vinblastine pour 1'obtention d'un blocage a 15 des cellules en mitose. Elle est equivalente a la vincristine et trois fois plus puis sante que la vinblastine pour un blocage a 50% (Bayssas and Barral 1978).…”