The action of serotonin in the rat hippocampal slice preparation.

Segal, Menahem

doi:10.1113/jphysiol.1980.sp013297

Cited by 195 publications

(70 citation statements)

References 26 publications

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“…When the ionophoresis was terminated, the depolarization was often followed by a long lasting hyperpolarization, generally accompanied by a decrease in membrane conductance, in agreement with similar results obtained in spinal motoneurones and in neurones of the olfactory cortex (Engberg et al 1979;. Such responses might be due at least in part, to an activation of the Na+-K+ pump (Segal, 1981), or possibly to a release of cathecholamines from the neighbouring monoaminergic fibres (Engberg et al 1979), which would explain the observed decrease in membrane conductance on the basis of the well known action of norepinephrine on Purkinje cell membranes (Siggins, Oliver, Hoffer & Bloom, 1971).…”

Section: Excitator Y Amino Acids In Cerebellar Slicessupporting

confidence: 76%

Effect of glutamate, aspartate and related derivatives on cerebellar Purkinje cell dendrites in the rat: an in vitro study

Crépel

Dhanjal

Sears

1982

The Journal of Physiology

198

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SUMMARY1. The responses ofPurkinje cells to short duration (pulse) ionophoretic applications of L-aspartate (L-asp), L-glutamate (L-glu), N-methyl DL-aspartate (NMDLA) and quisqualic acid in their dendritic fields were studied in vitro on sagittal slices of lobules IX and X of the adult rat cerebellum.2. Pulse application of L-asp or L-glu evoked transient and dose-dependent increases in the firing rate of the simple spikes recorded extracellularly as single units. When the ionophoretic electrode was positioned in the dendritic field of the Purkinje cells, the lowest thresholds for L-glu and L-asp mediated excitations of the cells were as low as 25 and 35 pC respectively, with a latency for maximal responses as brief as 7 ms.3. In intracellular recordings these excitatory responses consisted ofdepolarizations of up to 18 mV in amplitude and with depolarizing slopes up to 0-52 mV/ms. They were generally unaccompanied by changes in cell input resistance in contrast to the marked decrease which occurred in response to steady applications of large doses of L-asp and L-glu.4. The spatial distribution of the excitatory sites confirmed that the dendritic sensitivity to L-glu was greater than that of the soma and showed that the same was true for L-asp. In 34 % of cells the sensitivity for L-asp declined markedly in the upper region of the molecular layer, whereas it remained high for L-glu; no such differential sensitivity was detected in the remaining 66 % of cells. F. CREPEL, S. S. DHANJAL AND T. A. SEARS greater and its action more prolonged. Furthermore, its steady application led to an abrupt and marked decrease in cell membrane resistance.8. The excitatory effects of L-asp, L-glu and quisqualic acid were antagonized by L-glutamic acid diethyl ester more consistently than by D-oz-aminoadipate, suggesting together with previous observations that L-asp and L-glu act on Purkinje cells via quisqualic acid rather than via NMDLA receptors.

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Section: Excitator Y Amino Acids In Cerebellar Slicessupporting

confidence: 76%

Effect of glutamate, aspartate and related derivatives on cerebellar Purkinje cell dendrites in the rat: an in vitro study

Crépel

Dhanjal

Sears

1982

The Journal of Physiology

198

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“…Applications of micromolar concentrations of 5-HT exert multiple and well characterized actions on CAl pyramidal neurones through distinct 5-HT receptor subtypes (Anwyl, 1990). Postsynaptic actions of 5-HT on CAI pyramidal neurones include hyperpolarization of the cell membrane associated with decreased input resistance, and a reduction of the afterhyperpolarization (AHP; Segal, 1980;Andrade & Nicoll, 1987;Colino & Halliwell, 1987;Ropert, 1988). Activation of an inwardly rectifying potassium channel mediates the membrane hyperpolarization and decrease in input resistance, through a 5-HTlA receptor (Andrade & Nicoll, 1987;Colino & Halliwell, 1987) associated with a G protein (Andrade et al, 1986) The slow excitatory action and reduction of the amplitude of the AHP (Chaput et al, 1990;Andrade & Chaput, 1991) is due to the closing of calcium-dependent K+ channels (Imp; Andrade & Nicoll, 1987;Colino & Halliwell, 1987), supposedly through the activation of 5-HT4-like receptors (Chaput et al, 1990;Andrade & Chaput, 1991).…”

Section: Introductionmentioning

confidence: 99%

Effects of DAU 6215, a novel 5‐hydroxytryptamine₃ (5‐HT₃) antagonist on electrophysiological properties of the rat hippocampus

Passani

Pugliese

Azzurrini

et al. 1994

British J Pharmacology

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1 The aim of the present study was to test the effects of DAU 6215 (endo-N-(8-methyl-8-azabicyclo-[3.2.1]-octo-3-yl)-2,3-dihydro-2-oxo-lH-benzimidazole-1-carboxamide hydrochloride), a newly synthesized, selective aminophosphonovaleric acid (APV; 501M) and 6,7-dinitroquinoxaline-2,3-dione (DNQX; 25 tiM), and were elicited by the selective 5-HT3 agonist, 2-methyl-5-HT (2-Me-5-HT, 50 pM).5 The increase in frequency of s.p.s.ps induced by 5-HT was significantly antagonized by DAU 6215 in 70% of the cases, whereas the 5-HT3 antagonist always suppressed the effect of 2-Me-5-HT, at concentrations as low as 60 nM. 6 The antagonistic effect of DAU 6215 was also tested on the 5-HT3-mediated block of induction of long-term potentiation (LTP), elicited by a primed burst (PB) stimulation. Extracellular recordings showed that low concentrations (60 nM) of DAU 6215 suppressed the inhibitory action of 5-HT on PB-induced LTP, without affecting the 5-HTlA-induced reduction in the amplitude of the population spike. 7 These results provide evidence that DAU 6215 is an effective antagonist of the 5-HT3-mediated responses in the central nervous system and may offer a cellular correlate for the pharmacological effects of DAU 6215 as an anxiolytic and cognition enhancer.

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“…The advent of the slice preparation facilitated the study of neural interactions as it allows prolonged intracellular recording and control of the cellular environment. Thus, recent studies have characterized some properties of hippocampal neurones in vitro (Yamamoto, 1972;Schwartzkroin, 1975; Andersen, Sundberg, Sveen & Wigstrom, 1977;Segal, 1980).…”

Section: Introductionmentioning

confidence: 99%

Visualization of the spread of electrical activity in rat hippocampal slices by voltage‐sensitive optical probes

Grinvald

Manker

Segal

1982

The Journal of Physiology

234

123

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SUMMARY1. Voltage-sensitive membrane-bound dyes and a matrix of 100 photodetectors were used to detect the spread of evoked electrical activity at the CAI region of rat hippocampus slices. A display processor was designed in order to visualize the spread of electrical activity in slow motion.2. The stimulation of the Schaffer collateral-commissural path in the stratum radiatum evoked short latency (2-4 msec) fast optical signals, followed by longer latency (4-15 msec) slow signals which decayed within 20-50 msec. Multiple fast signals were frequently detected at the stratum pyramidale; they propagated toward the stratum oriens with an approximate conduction velocity of 0-1 m/sec.3. The fast signals were unaltered in a low Ca2+ high Mg2+ medium but were blocked by tetrodotoxin. These signals probably represent action potentials in the Schaffer collateral axons. Their conduction velocity was about 0-2 m/sec and their refractory period about 3-4 msec.4. The slow signals were absent in a low Ca2+ medium and probably represent excitatory post-synaptic potentials (e.p.s.p.s) generated in the apical dendrites of the pyramidal cells. They were generated in the stratum radiatum, where the presynaptic signals were seen, and spread into somata and basal dendrites (the stratum pyramidale and oriens, respectively).5. The timing of the signals with fast rise-time, which were detected at the status pyramidale, approximately coincided with the timing of the extracellularly recorded field potentials. These multiple discharges probably represent action potentials of the pyramidal cells. They spread back into the apical dendrites but with significant attenuation of the amplitudes of the high frequency components of the pyramidal action potentials.6. Hyperpolarizing potentials could be detected when strong stimuli were applied to the stratum radiatum or alveus. The net hyperpolarizations were detected only in the stratum pyramidale and the border region between the stratum pyramidale and radiatum. Frequently the inhibition was masked by the large e.p.s.p.s. However, its existence could be demonstrated by treatment of the slice with picrotoxin or a low Cl-medium. Under these conditions a long-lasting depolarization of the apical dedrites was evoked by the stimulation. This was associated with an increase of the multiple discharges in the stratum pyramidale and oriens.7. These studies illustrate the usefulness of voltage-sensitive dyes in the analysis A. GRINVALD, A. MANKER AND M. SEGAL of passive and active electrical properties, pharmacological properties and synaptic connexions in mammalian brain slices, at the level both of small neuronal elements (dendrites, axons) and of synchronously active neuronal populations.

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The action of serotonin in the rat hippocampal slice preparation.

Cited by 195 publications

References 26 publications

Effect of glutamate, aspartate and related derivatives on cerebellar Purkinje cell dendrites in the rat: an in vitro study

Effect of glutamate, aspartate and related derivatives on cerebellar Purkinje cell dendrites in the rat: an in vitro study

Effects of DAU 6215, a novel 5‐hydroxytryptamine₃ (5‐HT₃) antagonist on electrophysiological properties of the rat hippocampus

Visualization of the spread of electrical activity in rat hippocampal slices by voltage‐sensitive optical probes

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The action of serotonin in the rat hippocampal slice preparation.

Cited by 195 publications

References 26 publications

Effect of glutamate, aspartate and related derivatives on cerebellar Purkinje cell dendrites in the rat: an in vitro study

Effect of glutamate, aspartate and related derivatives on cerebellar Purkinje cell dendrites in the rat: an in vitro study

Effects of DAU 6215, a novel 5‐hydroxytryptamine3 (5‐HT3) antagonist on electrophysiological properties of the rat hippocampus

Visualization of the spread of electrical activity in rat hippocampal slices by voltage‐sensitive optical probes

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Effects of DAU 6215, a novel 5‐hydroxytryptamine₃ (5‐HT₃) antagonist on electrophysiological properties of the rat hippocampus