The action of secretin, cholecystokinin‐‐pancreozymin and caerulein on pancreatic secretion in the rat

Dockray, Graham J.

doi:10.1113/jphysiol.1972.sp009963

Cited by 112 publications

(75 citation statements)

References 20 publications

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“…In particular, caerulein and CCK share the same C-terminal heptapeptide (with the exception of a single substitution of methionine in CCK for threonine in caerulein), and their C-terminal pentapeptides are identical with those in gastrin. Since the C-terminal heptapeptide of CCK is the minimal fragment of the molecule possessing strong activity on the guinea pig gall bladder (13) and probably on the rat pancreas also (14), it is not surprising to find that caerulein is highly active in these two preparations (5,6). …”

Section: Discussionmentioning

confidence: 99%

“…The action of caerulein on pancreatic secretion in urethane-anesthetized rats was also used as a bioassay (6). In both the guinea pig and rat bioassays the actions of the skin secretions were compared with those of synthetic caerulein (Farmitalia Ltd., Milan, Italy) and 10% natural porcine CCK (Gastrointestinal Hormone Research Unit, Karolinska lnstitutet, Stockholm, Sweden).…”

Section: Stimulation Of Secretionmentioning

confidence: 99%

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Caerulein secretion by dermal glands in xenopus laevis

Dockray¹,

Cr²

1975

The Journal of Cell Biology

128

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Since gastrin and its related peptides are secreted by a minority population of widely dispersed cells in mammalian tissues it has, in the past, been difficult to study the subcellular aspects of their secretion. From published reports (I, 2) it seemed possible that a satisfactory system for such studies might be provided by the skin of certain amphibians such as Xenopus laevis since in these tissues high concentrations of peptides such as caerulein exist, and there is some indication (3) that this, or a similar gastrin-like peptide, may be a dermal gland secretory product. We have therefore explored this possibility by studying the structure, secretory process, and secretory product of the most prominent nonmucous type of gland in the skin of X.laevis. These studies clearly demonstrate that most, if not all, of the caerulein in the skin is contained in secretion granules within the dermal glands and that its release can be specifically evoked by adrenergic stimulation. The release process by a holocrine mechanism expels all of the stored secretion onto the skin surface and thus for 724

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Section: Discussionmentioning

confidence: 99%

Section: Stimulation Of Secretionmentioning

confidence: 99%

Caerulein secretion by dermal glands in xenopus laevis

Dockray¹,

Cr²

1975

The Journal of Cell Biology

128

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“…The relevance of in vitro studies is difficult to assess since most of the work has been done in rat and guinea-pig pancreas in which, unlike the dog and man, secretin increases protein secretion proportionally with dose (Dockray, 1972;Gardner & Jackson, 1977).…”

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confidence: 99%

The role of nicotinic receptors in dog pancreatic exocrine secretion

Devaux

Díaz

Kubota

et al. 1983

British J Pharmacology

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The effects of pentamethonium, an autonomic ganglion blocker, were studied on the exocrine pancreatic secretion of six conscious dogs given intravenous infusions of urecholine, caerulein or pentagastrin on a background of submaximal doses of secretin. 2 Urecholine-induced protein secretion was not affected but both caerulein-and to a smaller extent, pentagastrin-induced protein secretions were depressed by pentamethonium. 3 These results indicate that intravenous caerulein and pentagastrin, but not urecholine, act at least partially via nicotinic receptors. 4 Volume and bicarbonate output were depressed by pentamethonium when stimulated by intravenous caerulein with a background of secretin, but not when stimulated by pentagastrin on a background of secretin. 5 From these data it is suggested that caerulein and pentagastrin may potentiate secretinstimulated hydrelatic secretion by different mechanisms. IntroductionExocrine pancreatic secretion is regulated by both neural and hormonal mechanisms. The interaction on exocrine pancreatic secretion between cholinergic nerves and gastrointestinal hormones (GI hormones) has been investigated chiefly with atropine, which blocks muscarinic receptors. In spite of extensive studies, there still exists a diversity of opinion about the action of atropine and acetylcholine on exocrine pancreatic secretion (Thomas, 1964;Magee, Fragola & White, 1965;Henriksen, 1969;Konturek, Tasler & Obtulowicz, 1972; Vaysse, Bastie, Pascal, Roux, Martinel, Lacroix & Ribet, 1975). In addition, it is not clear whether or not nicotinic receptors play a part in the exocrine pancreatic secretory response to GI hormones.The purpose of this study was to examine the effects of an autonomic ganglionic blocker (pentamethonium) on the pancreatic secretion stimulated by urecholine, caerulein or pentagastrin and to elucidate the possible role of the nicotinic receptors on stimulated pancreatic secretion. MethodsSix male Beagle dogs, weighing 12-17 kg, were prepared with Thomas cannulae (Thomas, 1941) in both duodenum and stomach after preliminary ligation of the accessory pancreatic duct. Experiments were started no sooner than three weeks after surgery. Dogs were deprived of food but not water for at least 18 h before each test. The gastric cannula was kept open during tests. The dogs were restrained in Pavlov stands.The exocrine pancreas was stimulated by: (1) urecholine (carbamylmethylcholine chloride, Sigma) 200 gig/h, (2) caerulein (ceruletide, Farmitalia) 35 ng kglh-1, or (3) pentagastrin (Peptavlon, ICI) 3ygkg-1h-1. Caerulein was used in preference to cholecystokinin (CCK) because it can be much more readily and inexpensively obtained in pure form. Each stimulant dissolved in saline was infused on a background of GIH secretin 0.5 clinical unit kg-I h-l. Without secretin the volume of juice varies between zero and 1-3 ml/10 min. It is, therefore, impossible to monitor change in both directions. In order to give these stimulants continuously, a Venocath (G-21, Abbott) was placed in a leg vein and a ...

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“…The tissue weights of the rat and hamster pancreas were about 0.34% (Lewi and Marsboom 1981) and 0.5% of total body weight, respectively. The maximal flow rate of juice per unit pancreatic tissue weight in the hamster in response to the maximal dose of caerulein (Fig, l) was small (0.8 pl/min/g pancreas) compared with that in the rat pancreas in vivo in response to the maximal dose of pure pancreozymin (5.3 u 1/min/g pancreas, Dockray 1972) and in the mouse pancrease in vivo in response to the pilocarpine (about 10 jil/min/g, Mangos et al 1973). Thus, in the hamster pancreas the acinar cell stimulants evoke a smaller fluid secretion than in the mouse or rat pancreas.…”

Section: Discussionmentioning

confidence: 99%

“…wt.) which was near the maximal dose for fluid secretion in the rat (Dockray 1972). The tissue weights of the rat and hamster pancreas were about 0.34% (Lewi and Marsboom 1981) and 0.5% of total body weight, respectively.…”

Section: Discussionmentioning

confidence: 99%

Fluid secretion and electrical properties of pancreatic acini of Syrian Golden hamster.

Suzuki

1986

Tohoku J. Exp. Med.

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SuzuKI, Y. Fluid Secretion and Electrical Properties of Pancreatic Acini of Syrian Golden Hamster. Tohoku J. exp. Med., 1986, 150 (2), [189][190][191][192][193][194][195][196][197][198][199][200][201][202][203][204][205][206][207][208] Mechanisms of electrolyte and fluid secretion in the hamster pancreatic acini were investigated by measurements of fluid and amylase secretion in vivo and in vitro, and membrane potential and resistance in vitro. Unlike mouse and rat pancreas the hamster pancreas secreted only a small amount of juice in response to acinar stimulants such as acetylcholine (ACh) and caerulein. The mean resting potential was -62 mV and the mean resting input resistance was 29 M2. Ionophoretic application of ACh evoked membrane depolarization accompanied by a reduction in input resistance. The reversal potential for this ACh-evoked depolarization (EAch) was -11 mV. The EAch was shifted by changing extracellular K, Na and Cl concentration in a similar way to that of mouse pancreatic acinar cells. The relative permeability and current for K, Na and Cl at the EAch calculated by using the Goldman-Hodgkin-Katz equations were PK/PNa/Pc, =1/0.9/0.43 and IK/INa/ Ic, =1/1.4/0.4 respectively (In the mouse Pk/PNa/Pc, =1/1.2/5 and IK/INa/Ic, =1/ 2.3/1.3, Petersen et al. 1981). The results indicate that mechanisms of electrolyte and fluid secretion in hamster pancreatic acini are qualitatively similar to those in the mouse and rat pancreas. The less permeability of the acinar cell membrane to Cl ions in the hamster than in the mouse and rat appears to be associated with the fact that the acinar stimulant evokes a far smaller amount of fluid secretion in this species than in the mouse and rat.hamster ; pancreatic acinar cells ; fluid secretion ; electrical properties ; membrane permeabilityIn response to secretagogues such as acetylcholine (ACh) and pancreozymin pancreatic acinar cells secrete not only enzymes but also fluid with various secretory rate in different species. Species can be classified into two groups with respect to the degree of fluid secretion ; the first group includes the rat (Petersen and Ueda 1977) and mouse (Mangos et al. 1973) in which the pancreas secretes enzymes together with a large amount of pancreatic juice, and the second group includes the cat (Case et al. 1969) and man (Rinderknecht et al. 1978) in which the pancrease secretes enzyme with little juice.Electrical properties of pancreatic acinar cells can be also classified into two groups with respect to the response to secretagogues. In the mouse and rat

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The action of secretin, cholecystokinin‐‐pancreozymin and caerulein on pancreatic secretion in the rat

Cited by 112 publications

References 20 publications

Caerulein secretion by dermal glands in xenopus laevis

Caerulein secretion by dermal glands in xenopus laevis

The role of nicotinic receptors in dog pancreatic exocrine secretion

Fluid secretion and electrical properties of pancreatic acini of Syrian Golden hamster.

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