The action of phytotoxic substances of the fungusVerticillium dahliae on the permeability of a synthetic phospholipid membrane

Asamov, D. K.; Tursunkulova, R. Kh.; Isaev, P. I.; Otroshchenko, O. S.; Stepanichenko, N. N.

doi:10.1007/bf00567738

Cited by 3 publications

(7 citation statements)

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“…Phospholipids are the main components of the oomycete cell membrane, which is very important for maintaining cell membrane integrity and function [ 22 , 23 ]. In this study, the phospholipid content in the mycelia of LT263 and TA54 were detected.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Inhibitory Effect and Mechanism of Euphorbia Factor L3 against Phytophthora capsici

Zhang

Yang

et al. 2023

Molecules

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Phytophthora capsici is a highly destructive phytopathogenic oomycete with a broad host range and is responsible for tremendous losses. Euphorbia factor L3 (EFL3) is a natural plant-derived compound that has been widely studied in medicine and cosmetic applications. In this study, the sensitivity of 105 P. capsici isolates to EFL3 was determined, and the biological activity and physiological effects of EFL3 against P. capsici were investigated. The median effective concentration (EC50) values for EFL3 inhibition mycelial growth and spore germination ranged from 0.66 to 8.94 μg/mL (mean, 2.96 ± 0.91 μg/mL) and 1.63 to 13.16 μg/mL (mean, 5.30 ± 1.64 μg/mL), respectively. EFL3 treatment resulted in cell wall and cell membrane damage of P. capsici, which was revealed by morphological and ultrastructural observations, propidium iodide (PI) and calcofluor white (CFW) staining, and measurements of relative conductivity as well as malondialdehyde (MDA) and glycerol contents. In addition, the contents of phospholipid and cellulose, which are the major components of cell membrane and cell wall, were significantly reduced following EFL3 treatment. Furthermore, EFL3 provided protective as well as curative efficacies against P. capsici on detached tomato leaves and pepper seedlings in vivo. These data show that EFL3 exhibits strong inhibitory activity against P. capsici, thereby suggesting that it could be an effective alternative for controlling P. capsici-induced diseases.

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Section: Resultsmentioning

confidence: 99%

Evaluation of Inhibitory Effect and Mechanism of Euphorbia Factor L3 against Phytophthora capsici

Zhang

Yang

et al. 2023

Molecules

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“…Yet, the change of relative conductivity caused by compound 5b was much larger compared to the control (Figure b). According to previous reports, the cell membrane of fungi are the key action sites of the drugs, which can destroy the synthesis of phospholipid bilayer or protein and lead to the leakage of internal electrolytes. , Combined with the change of mycelial morphology after treatment with 5b , it can also be inferred that compound 5b could manifest antifungal properties by destroying the cell membrane structure . The above-reported findings revealed that the action target site of 5b on B. cinerea could be the cell membrane.…”

Section: Resultsmentioning

confidence: 85%

“…32,33 Combined with the change of mycelial morphology after treatment with 5b, it can also be inferred that compound 5b could manifest antifungal properties by destroying the cell membrane structure. 34 The above-reported findings revealed that the action target site of 5b on B. cinerea could be the cell membrane.…”

Section: ■ Results and Discussionmentioning

confidence: 90%

Antifungal Application of Rosin Derivatives from Renewable Pine Resin in Crop Protection

Pan

Jiang

et al. 2020

J. Agric. Food Chem.

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In the current work, we synthesized two series of dehydroabietyl amide derivatives from natural product rosin and evaluated their antifungal effects on Valsa mali, Phytophthora capsici, Botrytis cinerea, Sclerotinia sclerotiorum, and Fusarium oxysporum. In vitro and in vivo antifungal activities results indicated that rosin-based amide compounds containing thiophene heterocycles had better inhibitory effects on B. cinerea. In particular, compound 5b (5-fluoro-2-thiophene dehydroabietyl amide) exhibited the excellent antifungal properties against B. cinerea with an EC 50 of 0.490 mg/L, which was lower compared to the positive control penthiopyrad (0.562 mg/L). Physiological and biochemical studies showed that the primary action mechanism of compound 5b on B. cinerea changes mycelial morphology, increases cell membrane permeability, and inhibits the TCA pathway in respiratory metabolism. Furthermore, QSAR and SAR studies revealed that charge distribution of rosin-based amides derivatives have a key role in the antifungal activity through the hydrogen bonding, conjugation, and electrostatic interaction between the compounds and the receptors of the target. To sum up, this study contributes to the development of rosin-based antifungal agents with a novel structure and preferable biological activity.

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“…The change of relative conductivity indicates the variation of cell membrane permeability. According to literature, , the cell membrane of fungi is an important target for fungicides, which can inhibit the synthesis of a phospholipid bilayer or protein and cause the leakage of internal electrolytes. , As shown in Figure B, after treatment with compounds 13 and 25 , the mycelial relative conductivities of S. sclerotiorum, V. mali , and B.…”

Section: Resultsmentioning

confidence: 99%

“…According to literature, 34,36 the cell membrane of fungi is an important target for fungicides, which can inhibit the synthesis of a phospholipid bilayer or protein and cause the leakage of internal electrolytes. 37,38 As shown in Figure 6B, after treatment with compounds 13 and 25, the mycelial relative conductivities of S. sclerotiorum, V. mali, and B. cinerea increased with time, and the increase extent is much greater than that with the control, which indicates that 5. Compounds 13 and 25 have inhibitory effects on the respiration rate of B. cinerea mycelia, and their inhibition rates were 32.16 and 19.06%, respectively.…”

Section: Effects Of Compounds 13 and 25 On Cell Membranementioning

confidence: 93%

Bioactivity-Guided Synthesis Accelerates the Discovery of 3-(Iso)quinolinyl-4-chromenones as Potent Fungicide Candidates

Wang

Zhang

Wang

et al. 2020

J. Agric. Food Chem.

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Fungal infections could cause tremendous decreases in crop yield and quality. Natural products, including flavonoids and (iso)quinolines, have always been an important source for lead discovery in medicinal and agricultural chemistry. To promote the discovery and development of new fungicides, a series of 3-(iso)quinolinyl-4-chromenone derivatives was designed and synthesized by the active substructure splicing principle and evaluated for their antifungal activities. The lead optimization was guided by bioactivity. The bioassay data revealed that the 3-quinolinyl-4-chromenone 13 showed significant in vitro activities against S. sclerotiorum, V. mali, and B. cinerea with EC50 values of 3.65, 2.61, and 2.32 mg/L, respectively. The 3-isoquinolinyl-4-chromenone 25 exhibited excellent in vitro activity against S. sclerotiorum with an EC50 value of 1.94 mg/L, close to that of commercial fungicide chlorothalonil (EC50 = 1.57 mg/L) but lower than that of boscalid (EC50 = 0.67 mg/L). For V. mali and B. cinerea, 3-isoquinolinyl-4-chromenone 25 (EC50 = 1.56, 1.54 mg/L) showed significantly higher activities than chlorothalonil (EC50 = 11.24, 2.92 mg/L). In addition, in vivo experiments proved that compounds 13 and 25 have excellent protective fungicidal activities with inhibitory rates of 88.24 and 94.12%, respectively, against B. cinerea at 50 mg/L, while the positive controls chlorothalonil and boscalid showed inhibitory rates of 76.47 and 97.06%, respectively. Physiological and biochemical studies showed that the primary action of mechanism of compounds 13 and 25 on S. sclerotiorum and B. cinerea may involve changing mycelial morphology and increasing cell membrane permeability. In addition, compound 13 may also affect the respiratory metabolism of B. cinerea . This study revealed that compounds 13 and 25 could be promising candidates for the development of novel fungicides in crop protection.

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The action of phytotoxic substances of the fungusVerticillium dahliae on the permeability of a synthetic phospholipid membrane

Cited by 3 publications

References 0 publications

Evaluation of Inhibitory Effect and Mechanism of Euphorbia Factor L3 against Phytophthora capsici

Evaluation of Inhibitory Effect and Mechanism of Euphorbia Factor L3 against Phytophthora capsici

Antifungal Application of Rosin Derivatives from Renewable Pine Resin in Crop Protection

Bioactivity-Guided Synthesis Accelerates the Discovery of 3-(Iso)quinolinyl-4-chromenones as Potent Fungicide Candidates

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