The action of all-trans-retinoic acid (ATRA) and synthetic retinoid analogues (EC19 and EC23) on human pluripotent stem cells differentiation investigated using single cell infrared microspectroscopy

Abstract

(2013) 'The action of all-trans-retinoic acid (ATRA) and synthetic retinoid analogues (EC19 and EC23) on human pluripotent stem cells di erentiation investigated using single cell infrared microspectroscopy.', Molecular bioSystems., 9 (4). pp. 677-692. Further information on publisher's website:http://dx.doi.org/10.1039/c3mb25505kPublisher's copyright statement:Additional information: Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-pro t purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details.

Characterising cytotoxic agent action as a function of the cell cycle using fourier transform infrared microspectroscopy

“…25,26 Briefly, this algorithm creates a binary mask based on the quality test of the FTIR mosaic as a function of protein content; hence, the "blank" areas that do not correlate to a cell within the slide are not processed further. 25,26 Briefly, this algorithm creates a binary mask based on the quality test of the FTIR mosaic as a function of protein content; hence, the "blank" areas that do not correlate to a cell within the slide are not processed further.…”

Pharmacological evidence for the role of RAR in axon guidance and embryonic development of a protostome species

“…EC23 has previously been shown to exert similar effects to atRA in vertebrates, including the ability to induce neural differentiation (Christie et al, 2008;Maltman et al, 2009). Indeed, EC23 mimics atRA in inducing differentiation of motoneurons from human embryonic stem cells (Christie et al, 2010) as well as differentiation of neurons from pluripotent human embryonal carcinoma cell lines (Clemens et al, 2013).…”

“…We have demonstrated here for the first time, that the synthetic retinoid EC23, known to act at vertebrate RARs (but not RXRs; Gambone et al, 2002) can closely mimic the effects of RA in an invertebrate protostome species. Indeed, EC23 mimics atRA in inducing differentiation of motoneurons from human embryonic stem cells (Christie et al, 2010) as well as differentiation of neurons from pluripotent human embryonal carcinoma cell lines (Clemens et al, 2013). Indeed, EC23 mimics atRA in inducing differentiation of motoneurons from human embryonic stem cells (Christie et al, 2010) as well as differentiation of neurons from pluripotent human embryonal carcinoma cell lines (Clemens et al, 2013).…”

Photoactivated cell-killing involving a low molecular weight, donor–acceptor diphenylacetylene

“…Inspired by our work on the design, synthesis and applications of synthetic retinoids for controlling cellular development, [18][19][20][21][22][23][24][25] we recently designed uorescent analogues of such compounds for use in uorescence-based biophysical studies. 26,27 It is known that the addition of a strong p-donor (-NR 2 ) and strong p-acceptor (-CO 2 R) to a diphenylacetylene scaffold results in efficient charge transfer from the donor moiety to the acceptor moiety upon photoexcitation.…”