The acidogenic microbial composition of dental plaque from caries-free and caries-prone people

Hayes, Michael L.; Carter, E.C.; Griffiths, S

doi:10.1016/0003-9969(83)90132-2

Cited by 21 publications

(11 citation statements)

References 7 publications

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“…The results of this study are consistent with our previous study with adults (24). Although not a focus of the present study, one possible explanation for the lesser microbial diversity in S-ECC plaque is that caries plaques contain higher proportions of acidogenic and aciduric bacteria than caries-free plaques, as some research papers have reported (4,18,26,48). For S-ECC, several studies suggest that this form of caries results predominantly from a mutans streptococcus infection (3,5,27).…”

Section: Discussionsupporting

confidence: 92%

Genetic Profiling of the Oral Microbiota Associated with Severe Early-Childhood Caries

et al. 2007

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The determination of the composition of the microbial community in the oral cavity is usually based on cultivation methods; however, nearly half of the bacteria in the saliva and the dental plaque are not cultivable. In this study, we evaluated the difference in oral microbial diversity between children with severe early-childhood caries (S-ECC) and caries-free (CF) controls by means of a cultivation-independent approach called denaturing gradient gel electrophoresis (DGGE). Pooled dental plaque samples were collected from 20 children aged 2 to 8 years. Total microbial genomic DNA was isolated from those subjects, and a portion of the 16S rRNA gene locus was PCR amplified by using universal primers. We observed that the mean species richness of the bacterial population was greater in the CF children (n ‫؍‬ 12) (42 ؎ 3.7) than in the S-ECC children (n ‫؍‬ 8) (35 ؎ 4.3); the difference was statistically significant (P ‫؍‬ 0.005). The overall diversity of plaque samples as measured by the Shannon index was 3.5 for the S-ECC group and 3.7 for the CF group (P ‫؍‬ 0.004). Differences in DGGE profiles were distinguished on the basis of a cluster analysis. Sequence analysis of excised DGGE bands consisted of 2.7 phylotypes, on average. After adjusting for the number of observed bands, we estimated that the S-ECC group exhibited 94.5 total phylotypes and that the CF group exhibited 113.4. These results suggest that the microbial diversity and complexity of the microbial biota in dental plaque are significantly less in S-ECC children than in CF children.

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Section: Discussionsupporting

confidence: 92%

Genetic Profiling of the Oral Microbiota Associated with Severe Early-Childhood Caries

et al. 2007

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“…Scheie et al [1984] pointed out that the effect of the dietary sucrose regulation of S. mutans in plaque may not be especially pronounced in a short-term experiment. Our findings with raised acidogenicity without a significant increase in the species studied are supported by Hayes et al [1983], who found that a frequent sucrose intake up to 20 days can increase the acidogenic ratio of dental plaque but cannot cause any corresponding change in the total oral flora. Although this study was focused on MS and S. sanguis, it is important to remember that other acidogenic micro-organisms, such as non-MS, can be numerous in dental plaque and, even in cariesassociated plaques, frequently far outnumber MS [van Houte et al, 1991[van Houte et al, , 1994.…”

Section: Discussionsupporting

confidence: 78%

Influence of Short-Term Sucrose Exposure on Plaque Acidogenicity and Cariogenic Microflora in Individuals with Different Levels of Mutans Streptococci

Quiroz¹,

Lingström²,

Birkhed³

2003

Caries Res

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The aim was to study the short-term effect of a varying sucrose exposure on plaque acidogenicity and cariogenic microflora in two groups of subjects with different levels of mutans streptococci (MS). Eight subjects with low (<10⁴) and 8 with high (>10⁶) numbers of MS per millilitre saliva participated. Three 7-day test periods were conducted. During two of these, the subjects rinsed either 5 or 10 times daily with 10% sucrose; the third period without any mouth rinses served as control. The subjects refrained from oral hygiene during the last 3 days of each test period. On day 7, the following parameters were measured: plaque pH after a sucrose rinse, numbers of MS, Streptococcus sanguis and lactobacilli in saliva, percent MS in plaque and plaque index. The results revealed that plaque acidogenicity was more pronounced for the high-MS group compared to the low-MS group after all three test periods, i.e. lower resting pH, deeper pH falls and a lower final pH. For both groups, the greatest pH-lowering capacity of plaque was found after the period with 10 sucrose rinses/day. An increase in bacterial counts was noted for both groups during the test periods with the 5- and 10-time rinse regimen; this increase was larger for the high-MS group compared to the group with low MS counts. The highest plaque index was, irrespective of the test period, found for the high-MS group.

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“…A similar correlation be tween caries and the number of salivary S. mutans has been reported by Van Houte et al [1981] and Zickert et al [1983]. However, no significant correlation be tween caries and the number of S. mutans in saliva or plaque was observed by Edwardsson et al [1972], Rundgren and Ericson [1978], and Hayes et al [1983]. While Loesche et al [1975] found a positive correla tion between caries and the number of S. mutans iso lated from fissures or pooled interproximal plaque, they could not demonstrate a correlation between salivary S. mutans and caries.…”

Section: Discussionmentioning

confidence: 51%

Long-Term Exposure of Plaque to Zinc Citrate

et al. 1988

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The effect of 3 years’ continuous exposure to dentifrices containing different levels of sodium monofluorophosphate, with or without 0.5% zinc citrate, has been investigated on plaque growth inhibition. Additionally, changes in the plaque microflora during prolonged exposure to zinc have been monitored in children with high or low caries increments. Following long-term use the inhibitory effect of zinc citrate on plaque growth was fully maintained. There was no evidence that prolonged exposure to zinc caused shifts in the plaque population of Streptococcus mutans, Streptococcus sanguis, lactobacilli, or Actinomyces species. A positive association was observed between children with high caries increments and high numbers of S. mutans in plaque and saliva. Caries increments were also associated with total viable count per microgram plaque protein. A negative association was observed between combined caries increment and number of S. sanguis in plaque.

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The acidogenic microbial composition of dental plaque from caries-free and caries-prone people

Cited by 21 publications

References 7 publications

Genetic Profiling of the Oral Microbiota Associated with Severe Early-Childhood Caries

Genetic Profiling of the Oral Microbiota Associated with Severe Early-Childhood Caries

Influence of Short-Term Sucrose Exposure on Plaque Acidogenicity and Cariogenic Microflora in Individuals with Different Levels of Mutans Streptococci

Long-Term Exposure of Plaque to Zinc Citrate

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