The acid-induced aggregation of E. coli s-RNA

Millar, David B.; MacKenzie, Maxime

doi:10.1016/0006-291x(66)90558-4

Cited by 7 publications

(2 citation statements)

References 4 publications

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“…when filtered on Sephadex G100 with 0.1 A260 unit of [3H]tyrosyl-tRNAT3r IA as a marker, tRNATvr IB or tRNA'vr III (5 A2M units) gives one peak of optical density superimposable with the radioactive peak (not shown here). Nevertheless, it is possible that dimers or aggregates formed during chromatography then might be easily disrupted like the unstable, concentration-dependent aggregates characterized by Millar and Mackenzie (21).…”

Section: Fraction Numbermentioning

confidence: 99%

Study of tyrosine transfer ribonucleic acid modification in relation to sporulation in Bacillus subtilis

Menichi¹,

Heyman²

1976

J Bacteriol

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MATERIALS AND METHODS Strains. B. subtilis W168, B. subtilis W23 D obtained from R. H. Doi, and W23 S obtained from P. Schaeffer were stored as spore suspensions. Growth conditions. Cells were cultivated at 37 C with vigorous agitation on different media: nutrient broth medium (28); Penassay broth (Difco); SCM medium (11) (synthetic medium supplemented with Casamino Acids lDifco]); MS medium (29) (synthetic medium with 0.2% NH4Cl as a nitrogen source and 0.5% malate as a carbon source). MSS medium (8), synthetic sporulation medium supplemented with or without iron, contains (in grams per liter): sodium citrate, 2.0; sodium acetate, 2.0; glutamic acid, 2.0; NH4C1, 0.5; glycerol, 0.5. Iron was extracted from a 5 x-concentrated stock solution with chloroform-8-hydroxyquinoline by the method of Waring and Werkman (35). Before use, MnCl2 and (NO3)2Ca, filter sterilized, were added from stock solutions to give final concentrations of 10 ,uM and 0.5 mM, respectively. This medium was supplemented with or without 1 ml of 1 mM SO4Fe per liter, filter sterilized. Experimental cultures were inoculated from growing precultures in the same medium, themselves already having been inoculated with cells from overnight cultures at 30C in agar-nutrient broth medium.

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Section: Fraction Numbermentioning

confidence: 99%

Study of tyrosine transfer ribonucleic acid modification in relation to sporulation in Bacillus subtilis

Menichi¹,

Heyman²

1976

J Bacteriol

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“…At this temperature about half the secondary structure is gone as judged by absorbance data. As the Tm of native E. coli tRNA in 0.3 m KC1 is about 57-58° ( Millar and MacKenzie, 1966a) and other physical parameters (Millar and Steiner, 1966;Millar, 1966;Millar and MacKenzie, 1966b) are similar to those of yeast tRNA (Lindahl et al, 1965;Henley et al, 1966), a comparison of properties between the two tRNA's is justified. Since our values of s and [77] are determined at 20°, the reaction of formaldehyde with E. coli tRNA clearly produces a highly denatured molecule, probably unfolded.…”

Section: Discussionmentioning

confidence: 99%

Structure Stabilization in Escherichia coli Transfer Ribonucleic Acid^*

Millar¹,

MacKenzie²

1967

Biochemistry

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Formaldehyde treatment of Escherichia coli curves, and polarization of fluorescence thermal profiles B transfer ribonucleic acid (tRNA) appreciably changes indicate that (1) Watson-Crick hydrogen bonding hydrodynamic and optical properties. The increase is a determinant in the configuration and helix-coil in intrinsic viscosity, decrease in sedimentation coeffi-transition of native E. coli tRNA and (2) base stacking cient coupled with the change in absorbance melting contributes to the helical rigidity in native tRNA.

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Products of limited ribonuclease digestion of Escherichia coli soluble ribonucleic acid

Millar

Byrne

1967

Archives of Biochemistry and Biophysics

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The acid-induced aggregation of E. coli s-RNA

Cited by 7 publications

References 4 publications

Study of tyrosine transfer ribonucleic acid modification in relation to sporulation in Bacillus subtilis

Study of tyrosine transfer ribonucleic acid modification in relation to sporulation in Bacillus subtilis

Structure Stabilization in Escherichia coli Transfer Ribonucleic Acid^*

Products of limited ribonuclease digestion of Escherichia coli soluble ribonucleic acid

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The acid-induced aggregation of E. coli s-RNA

Cited by 7 publications

References 4 publications

Study of tyrosine transfer ribonucleic acid modification in relation to sporulation in Bacillus subtilis

Study of tyrosine transfer ribonucleic acid modification in relation to sporulation in Bacillus subtilis

Structure Stabilization in Escherichia coli Transfer Ribonucleic Acid*

Products of limited ribonuclease digestion of Escherichia coli soluble ribonucleic acid

Contact Info

Product

Resources

About

Structure Stabilization in Escherichia coli Transfer Ribonucleic Acid^*