The acetylcholinesterase as indicative of intoxication for pesticide in farmers of conventional and organic cultivation

Abstract

In this sense the objective of assessing the levels of pesticide poisoning in rural farmers of San Sebastian and take AL, using acetylcholinesterase enzymes Erythrocyte and plasma as biological indicator of intoxication. This is a prospective, transversal and descriptive variables analyzed were: year whose, sex, age group, education, location, condition, route of exposure. The data were acquired by collecting blood samples and socio demographic information of farmers. Was put as the determining factor the type of conventional and organic farming, and periods of drought and rainy. 56 volunteers were analyzed. The analyses were performed in the automatic biochemical Analyzer Cobas Integra 400 plus®. According to the results of the analyses, it was the largest number of individuals with reduced values of cholinesterase, specifically the Group of conventional farming, the period of greatest change index was in the rainy season, where the activity of AChE, expressive values presented in the city of São Sebastião, with 80% result of intoxicated, in the city of the foot – take, 21.73% over the same period. On analysis of the AChP, São Sebastião has obtained the highest number of contaminated with 18.75% and 30% respectively, in the District Take Foot stood between 10 and 21.73% of reduced levels of cholinesterase. This sets the organic system of cultivation, as the best alternative for prevention of future diseases, in addition to bringing quality of life for rural workers, as well as for consumers.

Toxicity Evaluation, Oxidative, and Immune Responses of Mercury on Nile Tilapia: Modulatory Role of Dietary Nannochloropsis oculata

“…Acetylcholinesterase (AChE) activity is considered a valuable tool for recognizing frequent and continuous toxicant exposure [ 31 ]. AChE is an enzyme that controls and regulates the transmission of nerve impulses within the cholinergic synapsis; the higher the toxicity and metal ions bioaccumulation, the lower its enzymatic response [ 5 ].…”

“…Acetylcholinesterase activity was estimated to evaluate organic poisoning as a biological indicator of intoxication [ 31 ]. The collected serum cholinesterase was measured following the manufacturer’s protocol of the Cobas c pack reagents using COBAS INTEGRA 400 plus analyzer Roche Diagnostics.…”

Efeitos dos agrotóxicos na saúde do agricultor familiar dos municípios de Capitólio e Pimenta - Minas Gerais: avaliação de biomarcadores colinesterase eritrocitárias e plasmática

“…Os organofosforados e carbamatos representam a principal classe de inseticidas envolvida nos casos de intoxicação. Eles inativam as enzinas da acetilcolinesterase plasmática e eritrocitária, causando elevação dos níveis de acetilcolina, levando à síndrome colinérgica aguda com surgimento de sinais e sintomas muscarínicos, nicotínicos e do Sistema Nervoso Central, sendo essas manifestações dependentes da dose e da via de exposição envolvida (YANKOVSKAYA et al, 2018;ALVES et al, 2021).…”

“…A inibição dessas enzimas por compostos organofosforados leva ao acúmulo de acetilcolina e o organismo passa a apresentar manifestações indesejáveis (PINTO, 2017). Os Anticolesterásicos atuam como inibidores da enzima acetilcolinesterase, acumulando acetilcolina nos receptores colinérgicos, fazendo com que a transmissão colinérgica pós-sináptica não cesse no momento adequado, levando à hiperestimulação (ARAÚJO et al, 2016;ALVES et al, 2021).…”