The accumulation of endogenous proline induces changes in gene expression of several antioxidant enzymes in leaves of transgenic Swingle citrumelo

Carvalho, Kênia Mara Baiocchi de; Campos, Marília Kaphan Freitas de; Domingues, Douglas Silva; Pereira, Luiz Filipe Protásio; Vieira, Luiz Gonzaga Esteves

doi:10.1007/s11033-012-2402-5

Cited by 145 publications

(71 citation statements)

References 62 publications

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“…In normal conditions the maximum proline content in K3653.2 obtained with K3545.6 showed no significant difference. Proline content increased significantly under drought and severe salt stress conditions in A. altissima seedlings, supporting its role as a protective agent under oxidative stress conditions [34]. Simple correlation between antioxidant enzyme and some growth characteristics shown in Table 4.…”

Section: Resultsmentioning

confidence: 68%

Impact of Salinity Stress on Proline Reaction, Peroxide Activity, and Antioxidant Enzymes in Maize (Zea mays L.)

Molazem

Bashirzadeh

2015

Pol. J. Environ. Stud.

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Salinity is one of the environmental limiting factors in agricultural production. For the aim of investigating the effects of salt stress on some physiological traits in eight varieties, including K3615/1, S.C704, B73, S.C302, Waxy, K3545/6, K3653/2, and Zaqatala in three salinity levels, Zero (control), 50, and 100 mM NaCl in three replicates for the factorial experiment was carried out in randomized complete block design. Treatments were planted in pots. During the experiment superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), chlorophyll a, chlorophyll b, LRWC, and proline were measured. Increasing salinity to 50 mM and 100 mM increased SOD in most varieties. Maximum SOD in 50 mM was seen in K3545/6 with 20.62 unit/min g fw, that with SC302 and B73 were no significant differences, but with other varieties had significant differences. In concentrations of 100 mM, there was no significant difference between varieties in ascorbate peroxidase (APX) enzyme. In these conditions maximum and minimum amount of (APX) was seen in Sc704 with 8.533 unit/min g fw and K3615.1 with 3.898 unit/min g fw, respectively. In concentrations of 100 mM, there was a significant difference between varieties in (CAT) enzyme. In these conditions maximum and minimum amounts of (CAT) were seen in Sc302 and Sc704, respectively. Between catalase with SOD (0.234) and proline (0.284) we found positive and significant correlation at the 5% level. Significant positive correlation between proline with ascorbate peroxidase, catalase, and superoxide dismutase were observed.

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Section: Resultsmentioning

confidence: 68%

Impact of Salinity Stress on Proline Reaction, Peroxide Activity, and Antioxidant Enzymes in Maize (Zea mays L.)

Molazem

Bashirzadeh

2015

Pol. J. Environ. Stud.

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“…The increase in proline synthesis in transgenic tobacco (Nicotiana tabacum) overproducing proline by elimination of feedback inhibition of P5CS, which catalyzes the proline biosynthesis, caused higher tolerance to its hyperosmotic stress (Hong et al 2000;Kishor et al 1995). Carvalho et al (2013) also demonstrated that proline could modify the expression of genes related to the plant responses to water deficit.…”

Section: Resultsmentioning

confidence: 97%

Improvement ofex vitroacclimatization of mulberry plantlets by supplement of abscisic acid to the last subculture medium

2017

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Mulberry (Morus sp.) of the family Moraceae is very economically important in Asian countries including Korea, because its leaf and fruit have been commercially used in sericulture and horticultural industries. Therefore it is necessary to develop the optimal production system for rapid and cost-effective propagation of mulberry. Our studies focused on establishing an acclimatization method for the successful plantlet production of new cultivar 'Cheongsu' which was transferred ex vitro after in vitro culture. In particular, effect of abscisic acid (ABA) addition into the last subculture medium on plantlet response to subsequent ex vitro transfer and its growth was investigated. During acclimatization, stomatal conductance and transpiration rate of ABA-pretreated plantlets were significantly lower than those of non-treated plantlets. Net photosynthetic rate of ABA-pretreated plantlets decreased after ex vitro transfer but increased after 14 days, and it was mostly higher than that of non-treated plantlets. Moreover, relative water content as well as chlorophyll contents and its ratio were also higher in ABA-pretreated plantlets. On the other hand, proline was considerably higher than in control plantlets. After 1 month of ex vitro transfer, survival rate of ABA-pretreated plantlets was 85.6%, which increased by 29.1% in comparison with control (56.5%). More vigorous growth was also observed in ABA-pretreated plantlets. From these results, it was found that application of ABA to the last subculture medium could improve acclimatization and promote survival of mulberry plantlets after ex vitro transfer, inducing water stress tolerance and alleviating abiotic stresses.Keywords Mulberry, Abscisic acid, Ex vitro transfer, Acclimatization, Water stress Introduction Mulberry (Morus sp.) is an economically important tree grown in India, China, Korea and several Asian countries where its foliage is used as food for silkworms in sericulture industry (Vijayan et al. 2012). It is also commercially valuable in the horticulture, food and cosmetic industries, in particular, its phytochemical and medicinal properties such as antioxidants (Yen et al. 1996) and hypoglycemia compounds (Kelkar et al. 1996) have been widely used as healthcare products. These days it is cultivated for fruit production, which is used for human consumption including jam, jelly, frozen desserts, pulp, juice and wine (Koyuncu 2004). Mulberry fruit is regarded as a traditional medicine for dysentery, constipation and avulsed teeth due to rich phenolic acids and flavonoids (Arfan et al. 2012; Lee and Bae 2011).Plant micropropagation has been widely applied to agriculture industry for large scale production of economically important and valuable species. But its commercial use is limited in many species due to the low plantlet survival rates during the acclimatization (Pospisilova et al. 2009a, b), which are known to be related to an abnormal morphology, anatomy and physiology under in vitro culture conditions (Dias et al. 2013a). Poor functioning of...

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“…The high level of proline is useful to protect against lipid peroxidation (Molinari et al, 2007) removing ROS directly or activating the antioxidant defense system (Carvalho et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

The presence of Microlobius foetidus cause changes in the antioxidant defense of Urochloa decumbens?

et al. 2015

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Urochloa decumbens (Stapf) R. D. Webster (Poaceae) is an exotic species with has spread rapidly through the Cerrado area of Pantanal, Mato Grosso do Sul, Brazil. It has covered the soil aggressively turning it into cultivated pastures. Thus, it has become a challenge to protect native areas due its capacity of exclusion of native species. It has been observed that Microlobius foetidus (Jacq.) M.Sousa & G.Andrade species (Fabaceae) shows a dominant pattern over the development of U. decumbens. This work shows that M. foetidus interfere on the natural growth of U. decumbens within 10 m ratio. Between 15 and 20 m, it was observed an increase of Importance Value index (IVI) and Relative cover (RC) values. It was also observed a variation on the antioxidant defense system of U. decumbens within 10m ratio from M. foetidus. The enzymes superoxide dismutase, catalase and peroxidase present higher levels of activity then those found for glutathione reductase. This data indicates that M. foetidus may have an effect on U. decumbens, increase the activity of antioxidant enzymes. This effect probably happens as means to neutralize the toxic effects of the oxygen generated due to the presence of allelochemicals, which increases oxidative stress.Keywords: antioxidant enzymes, signal grass, garlic stick, competition. A presença de

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The accumulation of endogenous proline induces changes in gene expression of several antioxidant enzymes in leaves of transgenic Swingle citrumelo

Cited by 145 publications

References 62 publications

Impact of Salinity Stress on Proline Reaction, Peroxide Activity, and Antioxidant Enzymes in Maize (Zea mays L.)

Impact of Salinity Stress on Proline Reaction, Peroxide Activity, and Antioxidant Enzymes in Maize (Zea mays L.)

Improvement ofex vitroacclimatization of mulberry plantlets by supplement of abscisic acid to the last subculture medium

The presence of Microlobius foetidus cause changes in the antioxidant defense of Urochloa decumbens?

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