The ?accessory body? of Cajal in the neuronal nucleus

Lafarga, Miguel; Hervás, José-Pablo; Santa-Cruz, M. C.; Villegas, Juan; Crespo, David Revuelta

doi:10.1007/bf00317942

Cited by 58 publications

(11 citation statements)

References 31 publications

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“…The ultrastructural aspect of the CB is similar to that of the dense fibrillar component of the nucleolus (1,8,20). The nucleolus and CB are both equally and specifically stained by the silver staining method (8,21,22). CBs are also known to contain fibrillarin, which is a protein constituent ofthe fibrillar region of the nucleolus (17) and has been thought to be topologically specific for the nucleolus and prenucleolar bodies.…”

Section: Discussionmentioning

confidence: 73%

“…Electron micrographs frequently depict a CB in close association with the nucleolus, sometimes appearing as a budding structure (8,19,20). The ultrastructural aspect of the CB is similar to that of the dense fibrillar component of the nucleolus (1,8,20). The nucleolus and CB are both equally and specifically stained by the silver staining method (8,21,22).…”

Section: Discussionmentioning

confidence: 99%

“…The latter finding is especially interesting because we observed earlier that the specific fluorescent staining ofp80-coilin can be reduced or abolished if the cell substrate was treated with RNase but not with DNase (2). The detection of high levels of U-snRNA in CBs suggests that the functions for CB may be closely associated with some aspect of snRNA metabolism.Electron micrographs seldom show more than one CB per section of the nucleus (1,8). Using anti-p80-coilin antibodies in indirect immunofluorescence (IIF) microscopy, we have shown in many cell lines that the number of CBs varies from cell to cell and ranges from zero to six (2).…”

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confidence: 99%

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Immunocytochemical analysis of the coiled body in the cell cycle and during cell proliferation.

Andrade

Tan

Chan

1993

Proc. Natl. Acad. Sci. U.S.A.

187

151

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Coiled bodies (CBs) are small, round structures found in the nucleoplasm ofmost eukaryotic cells. Human autoantibodies to a 80-kDa protein, p80-coilin, are immunohistologic markers for CBs. A polyclonal rabbit antiserum (R288) raised against recombinant p80-coilin was shown to have similar immunochemical properties as human autoantibodies and was used to analyze the expression of p80-coilinassoiated CBs in cell cultures synchronized by double thymidine block, nocodazole arrest, serum starvation, or hormonal deprivation. By employing thymidine block and nocodazole arrest of HeLa cells, CBs were observed in immunofuorescent studies to be largest in size in the S and G2 phases of the cell cycle. These large CBs might have coalesced into one or two such structures per cell from smaller and more numerous CBs of three to eight per cell during the mid G1 phase of the cell cycle. No CB-llke structures were observed in mitosis and early G1. However, immunoblotting analyses showed that the total amount of p80-coilin remained approximately the same throughout the cell cycle. When HeLa cells were separated into soluble and particulate fractions, p80-collin was detected predominantly in the soluble fraction in mitosis and early G1, whereas it was present predominantly in the particulate fraction in late G1, S, and G2 when structurally distinct CBs were observed. In the analysis of CBs in two experimental models of cell proliferation (reversal of 3T3 serum starvation and FRTL-5 thyrotropin deprivation), proliferating ceOls contained larger, brighter, and more numerous CBs as well as a >2-fold increase in the total amount of p80-coilin compared to that in quiescent cells. The expression of p80-coffin in quiescent cells induced to proliferate and the cyclic formation and breakdown of CBs might be consistent with the notion that CBs may be specialized centers related to the maturation ofmRNA, but this evidence is indirect and needs further definitive study.The coiled body (CB) is a round-to-oval organelle with a diameter of 0.5-1 ,um and has been observed in many eukaryotic nuclei, ranging from human to plant. It was probably first described in light microscopy as the accessory body by the Spanish cytologist Ramon y Cajal in the beginning of the century. In the late 1960s, the CB was characterized by electron microscopy as a morphological structure distinct from other nuclear bodies (1). CBs are noncapsular structures composed of meandering threads loosely arranged in a coiled fashion. Despite the ubiquity of the CB in eukaryotic cells, little is known about its nature and function.Recently we have described human autoimmune antibodies that recognize an 80-kDa protein in CBs, and the autoantigen has been named p80-coilin (2). Using immunoelectron microscopy, we have shown that p80-coilin is highly enriched in the CB and that this autoantibody can be used as a specific probe for the CB (2, 3). Several components are now known to be present in CBs. Sm antigen has been demonstrated in CBs in earlier studies (4,5). Recent st...

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Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Immunocytochemical analysis of the coiled body in the cell cycle and during cell proliferation.

Andrade

Tan

Chan

1993

Proc. Natl. Acad. Sci. U.S.A.

187

151

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“…Indeed, the size and shape of these nuclear structures, as well as the localization in the nucleus, strongly suggest that they are Cajal bodies. Under the electron microscope we observed nuclear bodies as packed, coiled thick threads freely localized in the interchromatin region of the nucleus or adjacent to the nucleolus, which strongly resemble the ultrastructure of Cajal bodies [4, 7, 18, 42]. Additional evidence that the identified structures belong to the Cajal bodies class was the specific and strong accumulation of snRNP-related molecules in their area.…”

Section: Discussionmentioning

confidence: 93%

snRNP: Rich Nuclear Bodies inHyacinthus orientalisL. Microspores and Developing Pollen Cells

Zienkiewicz

Bednarska

2009

International Journal of Cell Biology

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The aim of the present work was the characterization of nuclear bodies in the microspore and developing pollen cells of Hyacinthus orientalis L.. The combination of Ag-NOR, immunofluorescence and immunogold techniques was used in this study. The obtained results showed the presence of highly agyrophylic extranucleolar bodies in microspore and developing pollen cells, which were finally identified as Cajal bodies. In all cases, a strong accumulation of snRNP-indicating molecules including TMG cap, Sm proteins and U2 snRNA, was observed in the examined nuclear bodies. In contrast to their number the size of the identified structures did not change significantly during pollen development. In the microspore and the vegetative cell of pollen grains CBs were more numerous than in the generative cell. At later stages of pollen development, a drastic decrease in CB number was observed and, just before anthesis, a complete lack of these structures was indicated in both pollen nuclei. On the basis of these results, as well as our previous studies, we postulate a strong relationship between Cajal body numbers and the levels of RNA synthesis and splicing machinery elements in microspore and developing pollen cells.

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“…One of the best known is the Cajal body (CB), first detected by light microscopy of sliver-stained nucleolar accessory bodies in 1903 by the Spanish cytologist Ramon y Cajal (1). Later, the same structure was characterized by electron microscopy as a round threaded non-membranelimited structure measuring 0.2 to 1.0 µm in diameter and denominated coiled body (2)(3)(4). Today, the structure is called Cajal body in honor of Ramon y Cajal (5).…”

Section: Introductionmentioning

confidence: 99%

Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cells

Silva

Christofolini

Mortara

et al. 2004

Braz J Med Biol Res

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Cajal bodies (CB) are ubiquitous nuclear structures involved in the biogenesis of small nuclear ribonucleoproteins and show narrow association with the nucleolus. To identify possible relationships between CB and the nucleolus, the localization of coilin, a marker of CB, and of a set of nucleolar proteins was investigated in cultured PtK 2 cells undergoing micronucleation. Nocodazol-induced micronucleated cells were examined by double indirect immunofluorescence with antibodies against coilin, fibrillarin, NOR-90/hUBF, RNA polymerase I, PM/Scl, and To/Th. Cells were imaged on a BioRad 1024-UV confocal system attached to a Zeiss Axiovert 100 microscope. Since PtK 2 cells possess only one nucleolus organizer region, micronucleated cells presented only one or two micronuclei containing nucleolus. By confocal microscopy we showed that in most micronuclei lacking a typical nucleolus a variable number of round structures were stained by antibodies against fibrillarin, NOR-90/hUBF protein, and coilin. These bodies were regarded as CB-like structures and were not stained by anti-PM/Scl and anti-To/Th antibodies. Anti-RNA polymerase I antibodies also reacted with CB-like structures in some micronuclei lacking nucleolus. The demonstration that a set of proteins involved in RNA/RNP biogenesis, namely coilin, fibrillarin, NOR-90/hUBF, and RNA polymerase I gather in CB-like structures present in nucleolidevoid micronuclei may contribute to shed some light into the understanding of CB function.

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The ?accessory body? of Cajal in the neuronal nucleus

Cited by 58 publications

References 31 publications

Immunocytochemical analysis of the coiled body in the cell cycle and during cell proliferation.

Immunocytochemical analysis of the coiled body in the cell cycle and during cell proliferation.

snRNP: Rich Nuclear Bodies inHyacinthus orientalisL. Microspores and Developing Pollen Cells

Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cells

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