The acceleration by polylysine of the activation of factor X by factor IXa

Factor IXa Inhibition by Protease Nexin-2/Amyloid .beta.-Protein Precursor on Phospholipid Vesicles and Cell membranes

“…The enzymatic activity of human and porcine FlXa also was measured by its ability to activate human factor X using polylysine as an artifical surface (Schmaier et al, 1993;Lundblad & Roberts, 1982;Griffith et al, 1985;McCord et al, 1990). Factor IXa (4.45 nM) was incubated with factor X (400 nM) in 0.1 M triethanolamine and 0.1 M NaCl, pH 8.0, containing 0.1% poly(ethylene glycol) (Mr = 8000) and 60 nM polylysine for 40 min at 20-25 °C.…”

Protease Nexin-2/Amyloid β-Protein Precursor Inhibits Factor Xa in the Prothrombinase Complex

“…Measurement of Factor Xa Formation-The enzymatic activity of human FIXa was measured by its ability to activate human factor X using polylysine as an artifical surface (Schmaier et al, 1993(Schmaier et al, , 1995Lundblad and Roberts, 1982;Griffith et al, 1985;McCord et al, 1990). Factor IXa (4.45 nM) was incubated with factor X (400 nM) in 0.1 M triethanolamine, 0.1 M NaCl, pH 8.0, containing 0.1% polyethylene glycol (M r ϭ 8000), 0.2% bovine serum albumin, and 60 nM polylysine for 40 min at 20 -25°C.…”

Enhanced Plasmin Inhibition by a Reactive Center Lysine Mutant of the Kunitz-type Protease Inhibitor Domain of the Amyloid β-Protein Precursor

“…The K i values for the purified KPI domains and coagulation factor IXa were determined using a polylysine and polyethylene glycol based factor X activation assay (13,14,22,23). Factor Xa (1 nM) inhibitory activity was measured by incubation with the purified KPI domains (5-10 nM) in 0.1 M triethanolamine, pH 8.0, 0.1 M NaCl, containing 0.1% polyethylene glycol (M r ϭ 8000) and 100 g/ml bovine serum albumin in triplicate in 96-well microtiter plates.…”