The Absorption of Water by Gelatin

Lloyd, Dorothy Jordan; Pleass, Winnifred Bertha

doi:10.1042/bj0211352

Cited by 29 publications

(9 citation statements)

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“…22 The unique amino acid composition of gelatin is responsible for its unusual hydrogen bonding properties and hence a high water retention capacity. 23,24 The preparation of nanoparticles by the desolvation method has been well studied and documented. 25,26 The effect of various reaction parameters such as temperature and pH on nanoparticle size has been investigated.…”

Section: Introductionmentioning

confidence: 99%

The role of surface charge in the desolvation process of gelatin: implications in nanoparticle synthesis and modulation of drug release

Ahsan¹,

Rao²

2017

IJN

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The process of moving hydrophobic amino acids into the core of a protein by desolvation is important in protein folding. However, a rapid and forced desolvation can lead to precipitation of proteins. Desolvation of proteins under controlled conditions generates nanoparticles – homogeneous aggregates with a narrow size distribution. The protein nanoparticles, under physiological conditions, undergo surface erosion due to the action of proteases, releasing the entrapped drug/gene. The packing density of protein nanoparticles significantly influences the release kinetics. We have investigated the desolvation process of gelatin, exploring the role of pH and desolvating agent in nanoparticle synthesis. Our results show that the desolvation process, initiated by the addition of acetone, follows distinct pathways for gelatin incubated at different pH values and results in the generation of nanoparticles with varying matrix densities. The nanoparticles synthesized with varying matrix densities show variations in drug loading and protease-dependent extra- and intracellular drug release. These results will be useful in fine-tuning the synthesis of nanoparticles with desirable drug release profiles.

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Section: Introductionmentioning

confidence: 99%

The role of surface charge in the desolvation process of gelatin: implications in nanoparticle synthesis and modulation of drug release

Ahsan¹,

Rao²

2017

IJN

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“…With keratin (Jordan Lloyd & Marriott, 1934) and silk Coleman & Howitt, 1947) there is also an increase rather than a decrease in swelling at high pH values. With silk this increased swelling is accompanied by splitting up of the fibres, and is attributed to a weakening of the cohesive forces of the fibre.The swelling of gelatin in alkaline solutions up to pH 10 is reduced by the addition of sodium chloride or other appropriate salts, in the same manner as swelling in acid solutions, but at higher pH values the curves have an upward trend, and the suppression of swelling is less marked (Jordan Lloyd & Pleass, 1927, 1928. There is also some evidence that the nature of the anion has some influence on swelling; e.g.…”

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confidence: 99%

“…The swelling of gelatin in alkaline solutions up to pH 10 is reduced by the addition of sodium chloride or other appropriate salts, in the same manner as swelling in acid solutions, but at higher pH values the curves have an upward trend, and the suppression of swelling is less marked (Jordan Lloyd & Pleass, 1927, 1928. There is also some evidence that the nature of the anion has some influence on swelling; e.g.…”

mentioning

confidence: 99%

“…A number of swelling curves of gelatin in alkaline solutions have been reported (Jordan Lloyd, 1920, 1930Jordan Lloyd & Pleass, 1927, 1928 Loeb, 1921a, b, c, d; Kunitz, 1923-4;Pleass, 1930), but in general these do not extend above pH 12-0 and make no allowance for solution of the gelatin. A few aLkaline swelling curves of collagen have also been reported (Kaye & Jordan Lloyd, 1924a, b;Jordan Lloyd, Marriott & Pleass, 1933;Highberger, 1936), but in all these there are insufficient points to determine the exact shape of the curves above pH 11-0.…”

mentioning

confidence: 99%

“…There is also some evidence that the nature of the anion has some influence on swelling; e.g. sodium nitrate depresses swelling to a lesser extent than sodium chloride at the same molar concentration (Jordan Lloyd & Pleass, 1927, 1928 order to minimize solution of the gelatin. The pieces of gelatin were removed and lightly wiped with blotting paper.…”

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confidence: 99%

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The swelling of collagen in alkaline solutions. 1. Swelling in solutions of sodium hydroxide

Bowes

Kenten

1950

Biochemical Journal

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The swelling of collagen and other fibrous proteins in acid and alkaline solutions is governed by the osmotic pressure differences arising between the protein phase and the external solution as a result of the formation of protein salts-Donnan membrane effect- (Procter, 1914;Bolam, 1932;Donnan, 1933), and by the cohesion of the protein (Procter, 1914; Jordan Lloyd, 1920, 1938, i.e. the forces opposing swelling, such as interweaving ofthe fibres and intermolecular forces. In order to interpret the effect of pH changes on swelling it is, therefore, necessary to consider the influence ofthe acid or alkali both on the Donnan equilibria and on the cohesion ofthe protein.The swelling of collagen and gelatin in alkaline solutions has received much less attention than that in acid solutions. A number of swelling curves of gelatin in alkaline solutions have been reported (Jordan Lloyd, 1920, 1930Jordan Lloyd & Pleass, 1927, 1928 Loeb, 1921a, b, c, d; Kunitz, 1923-4;Pleass, 1930), but in general these do not extend above pH 12-0 and make no allowance for solution of the gelatin. A few aLkaline swelling curves of collagen have also been reported (Kaye & Jordan Lloyd, 1924a, b;Jordan Lloyd, Marriott & Pleass, 1933;Highberger, 1936), but in all these there are insufficient points to determine the exact shape of the curves above pH 11-0. They show, however, no definite decrease in swelling at high pH values corresponding to that occurring in acid solutions below pH 2-0. With keratin (Jordan Lloyd & Marriott, 1934) and silk Coleman & Howitt, 1947) there is also an increase rather than a decrease in swelling at high pH values. With silk this increased swelling is accompanied by splitting up of the fibres, and is attributed to a weakening of the cohesive forces of the fibre.The swelling of gelatin in alkaline solutions up to pH 10 is reduced by the addition of sodium chloride or other appropriate salts, in the same manner as swelling in acid solutions, but at higher pH values the curves have an upward trend, and the suppression of swelling is less marked (Jordan Lloyd & Pleass, 1927, 1928. There is also some evidence that the nature of the anion has some influence on swelling; e.g. sodium nitrate depresses swelling to a lesser extent than sodium chloride at the same molar concentration (Jordan Lloyd & Pleass, 1927, 1928

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Fortschritte auf dem Gebiete der Gerbereichemie und ‐technik 1928–1934

Herfeld

1935

Angewandte Chemie

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The Absorption of Water by Gelatin

Cited by 29 publications

References 1 publication

The role of surface charge in the desolvation process of gelatin: implications in nanoparticle synthesis and modulation of drug release

The role of surface charge in the desolvation process of gelatin: implications in nanoparticle synthesis and modulation of drug release

The swelling of collagen in alkaline solutions. 1. Swelling in solutions of sodium hydroxide

Fortschritte auf dem Gebiete der Gerbereichemie und ‐technik 1928–1934

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