The Absorption of Calcium as a Function of the Body Saturation with Calcium

Nicolaysen, R

doi:10.1111/j.1748-1716.1943.tb02047.x

Cited by 77 publications

(21 citation statements)

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“…Periods of growth, pregnancy, lactation, and dietary calcium deprivation may enhance intestinal calcium absorption both in man and experimental animals (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12). Although the mechanism whereby increased body calcium needs stimulate intestinal calcium absorption remains uncertain, it is known that vitamin D is required for this adaptive response to occur (2,5).…”

Section: Introductionmentioning

confidence: 99%

Effects of Dietary Calcium Restriction and Chronic Thyroparathyroidectomy on the Metabolism of [3H]25-Hydroxyvitamin D3 and the Active Transport of Calcium by Rat Intestine

Favus¹,

Walling²,

Kimberg³

1974

J. Clin. Invest.

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Section: Introductionmentioning

confidence: 99%

Effects of Dietary Calcium Restriction and Chronic Thyroparathyroidectomy on the Metabolism of [3H]25-Hydroxyvitamin D3 and the Active Transport of Calcium by Rat Intestine

Favus¹,

Walling²,

Kimberg³

1974

J. Clin. Invest.

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“…INTESTINAL CALCIUM ABSORPTION and its regulation by vitamin D status have been studied for over 60 years (26). The active metabolite that controls this process is 1,25-dihydroxyvitamin D 3 [1,25(OH) 2 D 3 ] (27).…”

mentioning

confidence: 99%

Vitamin D-inducible calcium transport and gene expression in three Caco-2 cell lines

Fleet

Eksir

Hance

et al. 2002

American Journal of Physiology-Gastrointestinal and Liver Physi

102

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The parental cell line (P) of Caco-2 cells and two clones, BBe and TC7, were studied at 11 days postconfluence to test the facilitated diffusion model of vitamin D-mediated intestinal calcium absorption (CaTx). Nuclear vitamin D receptor (nVDR) and calbindin D 9k (CaBP) were measured by Western blot; 1,25-hydroxyvitamin D 3 24-hydroxylase (CYP24), CaBP, plasma membrane Ca-ATPase (PMCA), and Ca transport channel (CaT1) mRNA levels were examined by RT-PCR; and net apical-to-basolateral CaTx was examined after treating cells with vehicle or 10 nM calcitriol for 8 (mRNA levels) or 48 h (protein, CaBP mRNA, CaTx). nVDR level was lowest in BBe (38% P value) and directly related to CYP24 induction (TC7 ϭ P, which were 1.56 times greater than BBe). nVDR was inversely related to the vitamin D-induced levels of CaT1 mRNA, CaBP mRNA, PMCA mRNA, and net CaTx, with the highest induction seen in BBe. Basal CaBP mRNA (86 times greater than P) and protein levels were highest in TC7 cells and were not associated with higher net CaTx, suggesting CaBP may not be rate limiting for CaTx in these cells.intestine; calcium absorption; calbindin D 9k; calcium transport channel; plasma membrane Ca-adenosinetriphosphatase INTESTINAL CALCIUM ABSORPTION and its regulation by vitamin D status have been studied for over 60 years (26). The active metabolite that controls this process is 1,25-dihydroxyvitamin D 3 [1,25(OH) 2 D 3 ] (27). To date, two models have been proposed to explain this process: the facilitated diffusion model (2) and the vesicular model (23). In the facilitated diffusion model, summarized in Fig. 1, the basal rate of calcium uptake and calcium extrusion from the absorptive epithelial cell is proposed to be sufficient to accommodate the elevated rate of transport seen after vitamin D stimulation. In contrast, mathematical modeling shows that intracellular diffusion is the rate-limiting, as well as the most vitamin D responsive, step. Calbindin D 9k has been proposed as the protein that binds calcium and facilitates its diffusion between the apical and basolateral surfaces of the cell (8). The level of calbindin D 9k in the intestine is closely correlated with the efficiency of calcium absorption (2), and thus this protein plays a central role in the facilitated diffusion model. In the vesicular model, the cell uses lysosomes to sequester calcium and facilitate its movement to the basolateral membrane for extrusion (24). While calbindin D 28k (the form found in avian intestine) has been associated with lysosomes (25), the role of calbindin in this model is less clear. In the past, we have tested the facilitated diffusion model using cultures of the colonic carcinoma cell line Caco-2 (9, 11, 12, 32).Caco-2 cells are a human colon adenocarcinoma cell line that can spontaneously differentiate in culture and acquire a small intestinal phenotype, e.g., they become polarized columnar epithelial cells, they form tight junctions and domes, and they express several markers that are unique to differentiated small intestinal epithelium...

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“…This would represent one example of the Nicolaysen mechanism, which induces the gut to absorb calcium according to the needs of the body (103). This interpretation is also supported by the fact that lower doses of EHDP which in the rat lead to an increase in calcium absorption instead of the decrease seen at larger doses, stimulate rather than inhibit 1,25(OH),D, synthesis (104).…”

Section: Experimental Basis For the Clinical Use Of Diphosphonates Inmentioning

confidence: 49%

Experimental basis for the clinical use of diphosphonates in paget's disease of bone

Fleisch

1980

Arthritis & Rheumatism

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The Absorption of Calcium as a Function of the Body Saturation with Calcium

Cited by 77 publications

References 4 publications

Effects of Dietary Calcium Restriction and Chronic Thyroparathyroidectomy on the Metabolism of [3H]25-Hydroxyvitamin D3 and the Active Transport of Calcium by Rat Intestine

Effects of Dietary Calcium Restriction and Chronic Thyroparathyroidectomy on the Metabolism of [3H]25-Hydroxyvitamin D3 and the Active Transport of Calcium by Rat Intestine

Vitamin D-inducible calcium transport and gene expression in three Caco-2 cell lines

Experimental basis for the clinical use of diphosphonates in paget's disease of bone

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