The absorption of 131I-labelled homologous and heterologous serum proteins fed orally to young rats

Bangham, D. R.; Terry, R. J.

doi:10.1042/bj0660579

Cited by 42 publications

(21 citation statements)

References 10 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Passage through the stomach may render some portion of the antibodies present in the milk unsuitable for transmission through the enterocytes and antibodies which pass out of the proximal small intestine into the ileum will not be transmitted (Morris & Morris, 1975). These are factors which contribute to the very low estimates of the amount of antibody passed into the circulation from orally fed doses, 7-5 % (Bangham & Terry, 1957) and 12 % (Jones, 1972). These estimates did not include antibody which had left the vascular compartment during the experiment.…”

Section: Discussionmentioning

confidence: 99%

“…It has been observed by many workers (Bangham & Terry, 1957;Brambell, Halliday & Hemmings, 1961;Jones, 1972) that only a small proportion of the antibodies or y-globulins given orally to young rats and mice reached the circulation intact. Jones (1972), working with 12-day rats, estimated that only 12 % of the labelled rat immunoglobulin G administered orally could be recovered intact in the circulation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Quantitative assessment of the transmission of labelled protein by the proximal and distal regions of the small intestine of young rats.

Morris¹,

Morris²

1976

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. The plasma volume in rats aged 15-16 days was measured by dilution analysis using homologous, 1251-labelled immunoglobulin G. A mean plasma volume of 5-53 ml./100 g and a mean blood volume of 8-01 ml./100 g were obtained.2. After the injection of labelled immunoglobulin G into the heart, homogenates of various abdominal organs and of the carcass were prepared. Labelled immunoglobulin G left the vascular compartment at a rate of about 9-10 %/hr over a 3 hr period. About 11 % of the labelled immunoglobulin G was catabolized in 2 hr.3. The data obtained from these studies was used to make quantitative estimates of the amount of intact immunoglobulin G transmitted from the proximal intestine and from the ileum after the intra-intestinal injection of 1000 ug of labelled immunoglobulin G. Homogenates of the experimental animals were prepared and it was estimated that over 40 % of the labelled immunoglobulin G was transmitted as intact protein from the proximal intestine. The results suggest that no intact immunoglobulin G was transmitted from the ileum, but about 15 % of the protein removed from the ileum was recovered in the whole body as degraded fragments precipitable with trichloroacetic acid.4. These observations are discussed in the context of the transmission of antibodies, and their relevance to the receptor hypothesis is considered.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantitative assessment of the transmission of labelled protein by the proximal and distal regions of the small intestine of young rats.

Morris¹,

Morris²

1976

The Journal of Physiology

View full text Add to dashboard Cite

show abstract

“…with the physiological studies of Bangham and Terry (1957), Brambell et al . (1958), and Jordan and Morgan (1968) .…”

Section: Route Of Transport Across Proximal Absorptive Cellsmentioning

confidence: 99%

“…Antibodies present in the colostrum and milk of the mother are absorbed from the small intestine in the suckling rat and transported functionally intact into the circulation . Several features of this transport (see reviews : Brambell, 1958: Brambell, , 1966: Brambell, , 1970Morris, 1968), most notably the high degree of selection for homologous IgG immunoglobulins (Halliday, a, 1958Bangham and Terry, 1957 ;Halliday and Kekwick, 1960 ;Morris, 1965 ;Jordan and Morgan, 1968), have led to the hypothesis first proposed by Bram-THE JOURNAL OF CELL BIOLOGY • VOLUME 58, 1973 • pages 189-211 bell et al . (1958) that transport is mediated by the selective binding of immunoglobulins to receptor sites present in the absorptive cells of the small intestine .…”

Section: Introductionmentioning

confidence: 99%

Intestinal Transport of Antibodies in the Newborn Rat

Rodewald

1973

The Journal of Cell Biology

325

162

View full text Add to dashboard Cite

Evidence has been reported that the proximal small intestine of the neonatal rat selectively transports antibodies into the circulation . This study describes the morphology of the absorptive epithelial cells in this region of the intestine and their transport of several immunoglobulin tracers : ferritin-conjugated immunoglobulins (IgG-Ft) and antiperoxidase antibodies . Cells exposed to rat IgG-Ft bound the tracer on the membrane of tubular invaginations of the apical cell surface . Tubular and coated vesicles within the cell also contained the tracer, as did the intercellular spaces . Uptake of tracer was highly selective and occurred only with rat or cow IgG-Ft ; when cells were exposed to chicken IgG-Ft, ferritin-conjugated bovine serum albumin, or free ferritin, tracer did not enter the cell or appear in the intercellular spaces . Experiments with rat and chicken antiperoxidase showed a similar selective uptake and transport of only the homologous antibody . When cells from the distal small intestine were exposed to the tracers, all tracers were absorbed nonselectively but none were released from the cells . Cells from the proximal small intestine of the 22-day-old rat failed to absorb even rat IgG-Ft . A model is presented for selective antibody transport in proximal cells of the neonatal rat in which antibodies are selectively absorbed at the apical cell surface by pinocytosis within tubular vesicles . The antibodies are then transferred to the intercellular space within coated vesicles . Distal cells function only to digest proteins nonselectively .

show abstract

“…Antibodies in maternal colostrum or milk are transported across the intestinal mucosa into intestinal lymphatics (3). It has been demonstrated that this process is selective, shows species specificity, can be competitively inhibited, and is associated with protein catabolism (4)(5)(6)(7)(8)(9)(10). Based on these experimental findings an hypothesis for the mechanism of intestinal protein transport in the neonatal rat has been put forward (2,6,11,12).…”

Section: Introductionmentioning

confidence: 99%

The mechanism of intestinal uptake and transcellular transport of IgG in the neonatal rat

Jones¹,

Waldmann²

1972

J. Clin. Invest.

259

152

View full text Add to dashboard Cite

The absorption of 131I-labelled homologous and heterologous serum proteins fed orally to young rats

Cited by 42 publications

References 10 publications

Quantitative assessment of the transmission of labelled protein by the proximal and distal regions of the small intestine of young rats.

Quantitative assessment of the transmission of labelled protein by the proximal and distal regions of the small intestine of young rats.

Intestinal Transport of Antibodies in the Newborn Rat

The mechanism of intestinal uptake and transcellular transport of IgG in the neonatal rat

Contact Info

Product

Resources

About