The absorbance coefficient of beef heart cytochrome c1

Mechanism of Thiosulfate Oxidation in the SoxA Family of Cysteine-ligated Cytochromes

“…TsdA was further purified on a Superdex75 (16/60) preparative grade size exclusion column (GE Healthcare) that had been equilibrated in 30 m m Tris-HCl, pH 8.0, 160 m m NaCl. TsdA concentrations were determined from the heme concentration measured by the pyridine hemochrome method ( 23 ).…”

Biochemical and Molecular Studies of Mitochondrial Function in Diabetes Insipidus, Diabetes Mellitus, Optic Atrophy, and Deafness

“…Studies of oxidative flux through the respiratory chain were performed within 2 h of the mitochondrial preparation, using pyruvate and malate, succinate and oxoglutarate as substrates, and potassium ferricyanide as the electron acceptor (18). The mitochondrial cytochromes aa^, b, and c were measured from the reduced minus oxidized spectra at the temperature of liquid nitrogen using the extinction coefficients given by Tervoort et al (19). The remaining mitochondrial fraction was frozen at -85°C.…”