The absence of significant mutational events of the p53 gene in the only two salivary gland tumors possessing radiation-related development risks, mucoepidermoid carcinoma and Warthin tumor

To evaluate age-related differences in the expression of heat shock protein (HSP) 27 in periodontal ligament (PDL) cells at the protein and mRNA levels, we obtained PDL cells from the incisors of rats weighing approximately 150 g (young group) and 350 g (aged group) and seeded the cells in culture dishes. Over 2 weeks of cultivation, PDL cells were subjected to quantitative reverse transcription polymerase chain reaction (RT-PCR) to measure HSP27 mRNA, and HSP27 protein expression was examined by immunofluorescence. HSP27 mRNA expression in the young group increased slightly over the 2 weeks, while in the aged group it increased significantly over the same time period. The expression ratio of HSP27 mRNA in the aged group was higher than in the young group at 14 days. Furthermore, immunofluorescence signals for HSP27 were stronger in the aged group than in the young group. These data reveal that PDL cells vary their expression of HSP27 with age, and suggest that in view of such changes in HSP27 mRNA expression, the self-defense mechanism is enhanced in aged PDL cells although their overall homeostatic conditions are suppressed.

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Age-related differences in the expression of heat shock protein 27 by rat periodontal ligament cells in culture

Matsuzaka

Kokubu

Ito

et al. 2010

Oral Med Pathol

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Effect of mechanical loading on cultured osteogenic cells derived from different stages of bone wound healing in rats: experimental models for immediate or early implantation

et al. 2010

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Abstract:The purpose of this study was to evaluate the effects of mechanical loading on cultured osteogenic cells from different stages of bone wound healing. A bone cavity was created in a rat femur, and cells were obtained from the cavity during various phases of wound healing: the blood clot (BC) stage, the development of granulation tissue (GT), and callus formation (CF). After 7 days of primary culture, mechanical loading was applied once a day. Cells without mechanical loading were used as controls. Cell proliferation, alkaline phosphatase (ALP) activity and expression of mRNAs of both bone sialoprotein (BSP) and osteopontin (OPN) were evaluated. Cell proliferation of BC cells was significantly inhibited by mechanical loading. However, GT cells were significantly up-regulated by ALP activity at day 5, as were the expressions of BSP and OPN mRNAs at day 7 by mechanical loading. These results suggest that cells in bone granulation tissues have the ability to transduce mechanical stress to osteogenic differentiation.

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The absence of significant mutational events of the p53 gene in the only two salivary gland tumors possessing radiation-related development risks, mucoepidermoid carcinoma and Warthin tumor

Cited by 2 publications

References 52 publications

Age-related differences in the expression of heat shock protein 27 by rat periodontal ligament cells in culture

Age-related differences in the expression of heat shock protein 27 by rat periodontal ligament cells in culture

Effect of mechanical loading on cultured osteogenic cells derived from different stages of bone wound healing in rats: experimental models for immediate or early implantation

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