The absence of introns within a human fibroblast interferon gene

Houghton, Michael; Jackson, Ian J.; Porter, Alan G.; Doel, S. M.; Catlin, Graham; Barber, C. E.; Carey, N. H.

doi:10.1093/nar/9.2.247

Cited by 86 publications

(32 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this context it is intriguing that the genes for both a-and 3-interferons lack intervening sequences (17,22,36,48). These genes must produce functional mRNAs under the stress of viral infection, which, interestingly, is usually accompanied by fever in the intact organism.…”

Section: Resultsmentioning

confidence: 99%

Heat shock alters nuclear ribonucleoprotein assembly in Drosophila cells.

Mayrand¹,

Pederson²

1983

Mol. Cell. Biol.

View full text Add to dashboard Cite

Heterogeneous nuclear RNA is normally complexed with a specific set of proteins, forming ribonucleoprotein particles termed hnRNP. These particles are likely to be involved in mRNA processing. We have found that the structure of hnRNP is profoundly altered during the heat shock response in Drosophila cultured cells. Although hnRNA continues to be synthesized at a near-normal rate during heat shock, its assembly into hnRNP is incomplete, as evidenced by a greatly decreased protein content of the particles in Cs2SO4 density gradients. RNA-protein cross-linking conducted in vivo (Mayrand and Pederson, Proc. Natl. Acad. Sci. U.S. A. 78:2208-2212, 1981) also reveals that hnRNA made during heat shock is complexed with greatly reduced amounts of protein. The block of hnRNP assembly occurs immediately upon heat shock, even before the onset of heat shock protein synthesis. Additional experiments reveal that hnRNP assembled normally at 25°C subsequently disassembles during heat shock. The capacity for normal hnRNP assembly is gradually restored after heat-shocked cells are returned to 25°C. Heat-shocked mammalian cells also show a similar block in hnRNP assembly. We suggest that incomplete assembly of hnRNP during heat shock leads to abortive processing of most mRNA precursors and favors the processing or export (or both) of others whose pathway of nuclear maturation is less dependent on, or even independent of, normal hnRNP particle structure. This hypothesis is compatible with a large number of previous observations. Exposure of Drosophila embryos or cultured cells to elevated temperature elicits major changes in gene transcription and protein synthesis (2). Transcription of a small set of genes is activated by heat shock (4, 11), and the resulting heat shock mRNAs are rapidly exported to the cytoplasm where they are preferentially translated (19, 29, 47). Meanwhile, the appearance of new non-heat shock mRNAs in the cytoplasm ceases (46). It is not known whether this latter effect of heat shock reflects decreased rates of transcription, a reduced efficiency of mRNA processing, or both. We have previously reported that the rate of actin mRNA transcription rapidly decreases to a low level during heat shock in Drosophila cultured cells, suggesting that this particular mRNA may be regulated chiefly at the transcriptional level (11). However, there are other observations which indicate that transcription of heterogeneous nuclear RNA (hnRNA) continues during heat shock. First, although the rates of heat shock gene transcription are very high, these mRNAs nevertheless are found to comprise a surprisingly small fraction (2%) of the nonribosomal nuclear t Paper no. 22 in a series entitled "Ribonucleoprotein Organization of Eukaryotic RNA."RNA that is synthesized during heat shock (11). Second, the size distribution of nonribosomal nuclear RNA made during heat shock is heterogeneous and is similar to that of nuclear RNA from non-heat-shocked Drosophila cells (23)(24)(25)(26). In the present investigation we examined hnRNA transcr...

show abstract

Section: Resultsmentioning

confidence: 99%

Heat shock alters nuclear ribonucleoprotein assembly in Drosophila cells.

Mayrand¹,

Pederson²

1983

Mol. Cell. Biol.

View full text Add to dashboard Cite

show abstract

“…Whether the increased levels of/3 mRNA in the absence ofprotein synthesis can be explained entirely by the increase in the half-life of the /3 mRNA (5) or whether in the presence of cycloheximide the rate of transcription is altered as well (28) (29,30). It is interesting to note, but may only be coincidental, that both interferon and histone mRNAs belong to the rare class of unspliced mRNAs (31)(32)(33).…”

Section: Resultsmentioning

confidence: 99%

Analysis of interferon mRNA in human fibroblast cells induced to produce interferon.

Raj¹,

Pitha²

1981

Proc. Natl. Acad. Sci. U.S.A.

135

View full text Add to dashboard Cite

The levels of interferon mRNA as a function of interferon induction by poly(rI)-poly(rC) in human fibroblast cells were determined by RNA hybridization using a cloned 13 interferon cDNA and by translation in Xenopus oocytes. Whereas previous studies analyzed mixtures of interferons, the availability of the cloned 13 interferon cDNA and the antiserum to purified 13 interferon enabled us to focus on the expression of only one class (13) of interferon genes. The induction of interferon synthesis depends primarily on the accumulation of interferon 13 mRNA in the cells, and the interferon 13 mRNA rapidly disappears several hours after its appearance in the cytoplasm. No detectable interferon 13 mRNA sequences are present in uninduced cells. The degradation ofinterferon 13 mRNA in the induced cells requires ongoing protein synthesis; accumulation of interferon 13 mRNA was observed in the continuous presence of cycloheximide. The interferon 13 mRNA detected at the early stages of induction is 1100 nucleotides long and its size progressively decreases with time. By both the hybridization and the translational assay in Xenopus oocytes, only one size of interferon 13 mRNA and one species of 13 interferon could be identified.

show abstract

“…No strong structural relationship between the IFN-~/fl genes and the IFN-~ gene could be detected. Furthermore, the IFN-~ gene contains three introns , whereas the IFN-~/fl genes do not have introns 0000-6392 © 1985 SGM Goeddel et al, 1981 ;Tavernier et al, 1981 ;Houghton et al, 1981 ;Higashi et al, 1983;Shaw et al, 1983;E. C. Zwarthoffet al, unpublished results).…”

Section: Introductionmentioning

confidence: 97%

“…Most data published so far show that both the human genome and the mouse genome contain one copy of the IFN-fl gene (Houghton et al, 1981 ;Tavernier et al, 1981 ;Higashi et al, 1983), one copy of the IFN-~ gene ) and a series of over ten IFN-~ genes Bracket al, 1981 ;Goeddel et al, 1981 ;Owerbach et al, 1981 ;Shaw et al, 1983). The structure of the human and mouse IFN-fl (Taniguchi et al, 1980a;Derynck et al, 1980;Higashi et al, 1983), IFN-~, and (part of) the IFN-cc genes Streuli et al, 1980;Mantei et al, 1980;Goeddel et al, 1981;Ullrich et aL, 1982;Shaw et al, 1983;E.…”

Section: Introductionmentioning

confidence: 99%

Mouse Interferon Alpha Beta Genes Are Linked at the Centromere Proximal Region of Chromosome 4

Korput¹,

Hilkens²,

Kroezen³

et al. 1985

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYIn order to determine the chromosomal localization of the murine interferon-co (MuIFN-a) and murine interferon-fl (MuIFN-fl) genes the DNAs of a panel of somatic cell hybrids were analysed by Southern blot hybridization. The hybrid cells were derived from E36 Chinese hamster cells and GRSL or GR MaTu mouse cells and retained all hamster chromosomes but segregated mouse chromosomes. The MuIFN-~ probe used was a 0.7 kb HindIII-EcoRI fragment derived from the MuIFN-al gene which hybridized with both mouse and hamster DNA. However, four fragments present in EcoRI digests of mouse DNA were clearly absent from the hybridization profile of EcoRI-digested hamster DNA and could be used for detection of MuIFN-a sequences in the hybrid cells. The MuIFN-/~ probe, a 0.5 kb BglII-BamHI fragment derived from the MuIFN-fl gene, hybridized with a 2-6 kb EcoRI fragment of mouse DNA and only weakly cross-hybridized with a 4.8 kb EcoRI fragment in hamster DNA. Southern blot analysis of DNA from mouse/hamster hybrids compared with the analysis of chromosome markers showed that both the MuIFN-cc and the MulFN-fl genes are located on chromosome 4. Analysis of DNA from hybrids that contained only part of chromosome 4 indicated that the MuIFN-~ gene family and the MuIFN-fl gene are situated at the centromere-proximal region of the chromosome.

show abstract

The absence of introns within a human fibroblast interferon gene

Cited by 86 publications

References 47 publications

Heat shock alters nuclear ribonucleoprotein assembly in Drosophila cells.

Heat shock alters nuclear ribonucleoprotein assembly in Drosophila cells.

Analysis of interferon mRNA in human fibroblast cells induced to produce interferon.

Mouse Interferon Alpha Beta Genes Are Linked at the Centromere Proximal Region of Chromosome 4

Contact Info

Product

Resources

About