The absence of high-risk human papillomavirus in Czech non-small cell lung cancer cases

Jaworek, Hana; Koudeláková, Vladimíra; Slavkovský, Rastislav; Drábek, Jir̆ı́; Hajdúch, Marián

doi:10.5507/bp.2018.079

Cited by 7 publications

(4 citation statements)

References 21 publications

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“…The primary search generated 34 potentially relevant articles, six of which met the inclusion criteria ( 32 - 37 ) (Supplemental Material (Supplementary material) ). Two studies ( 38 , 39 ) did not find HPV in NSCLC patients whether or not they had EGFR mutations, and therefore could not be included in the meta-analysis.…”

Section: Methodsmentioning

confidence: 99%

Infection with human cytomegalovirus, Epstein-Barr virus, and high-risk types 16 and 18 of human papillomavirus in EGFR-mutated lung adenocarcinoma

et al. 2023

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Aim To assess the frequency of human cytomegalovirus (HCMV), Epstein-Barr virus (EBV), and high-risk types of human papillomavirus (HPV16 and HPV18) infections in lung adenocarcinoma samples. Methods Lung adenocarcinoma cytological smears and their DNA isolates were obtained from patients hospitalized at the Department for Lung Diseases Jordanovac, Zagreb, in 2016 and 2017. Overall, 67 lung adenocarcinoma samples were examined: 34 with epidermal growth factor receptor gene ( EGFR ) mutations and 33 without EGFR mutations. The EGFR mutation status and virus presence were assessed with a polymerase chain reaction, and random samples were additionally tested for EBV with Sanger sequencing. HCMV, EBV, HPV16, and HPV18 infections were evaluated in relation to EGFR mutation, smoking status, and sex. A meta-analysis of available data about HPV infection in non-small cell lung cancer was performed. Results More frequent HCMV, EBV, HPV16, and HPV18 infections were observed in lung adenocarcinoma samples with EGFR mutations than in samples without these mutations. Coinfection of the investigated viruses was observed only in lung adenocarcinoma samples with mutated EGFR . In the group with EGFR mutations, smoking was significantly associated with HPV16 infection. The meta-analysis showed that non-small cell lung cancer patients with EGFR mutations had a higher odds of HPV infection. Conclusion HCMV, EBV, and high-risk HPV infections are more frequent in EGFR -mutated lung adenocarcinomas, which indicates a possible viral impact on the etiology of this lung cancer subtype.

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Section: Methodsmentioning

confidence: 99%

Infection with human cytomegalovirus, Epstein-Barr virus, and high-risk types 16 and 18 of human papillomavirus in EGFR-mutated lung adenocarcinoma

et al. 2023

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“…The PCR primers and probes listed in Table 1 were synthesized by Generi Biotech Ltd. (Hradec Kralove, the Czech Republic). The qPCR analysis was performed using a Light Cycler 480 II (Roche Diagnostics, Mannheim, Germany) according to a previously described protocol [16].…”

Section: Methodsmentioning

confidence: 99%

Pitfalls of commercially available HPV tests in HPV68a detection

et al. 2019

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Background Human papillomavirus 68 (HPV68) is a probable carcinogenic HPV genotype which is included in almost all HPV screening assays and exists as two genetically variable subtypes (HPV68a and HPV68b). Routine HPV sample testing has shown that the cobas 4800 HPV Test (Roche) provides higher false-negative rates for HPV68 status than PapilloCheck HPV-Screening (Greiner Bio-One). The aim of our study was to evaluate the efficacy of cobas 4800 in HPV68 detection. Methods A total of 2,145 cervical/cervicovaginal samples from women aged 17–88 were tested for HPV68 status using the cobas 4800 and PapilloCheck HPV tests. Viral load was assessed by quantitative PCR in all of the HPV68-positive cases. HPV68a/b subtyping was performed with real-time PCR followed by high resolution melting curve analysis, and was subsequently confirmed by Sanger sequencing. Results Cobas 4800 detected HPV positivity in only 13/33 HPV68 single-genotype infection cases. Viral load was comparable across both tested subgroups. HRM analysis and Sanger sequencing identified the HPV68a subtype in all of the 20 instances of cobas 4800 false negatives. HPV68a and HPV68b were detected in 3/13 and 10/13 cases identified as other HPV-positive by cobas 4800. Conclusion The HPV68a subtype was missed by cobas 4800 in more than 85% of all HPV68a-positive cases. Therefore, commercially available assays may underestimate HPV68 prevalence.

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“…When the HPV was analyzed by conducting a consensus PCR, only five out of one hundred and thirty-two (3.8%) samples were HPV-positive [ 106 ]. In the Czech Republic, Jaworek et al (2020) did not detect HPV in 80 primary NSCLCs by using qPCR [ 107 ]. In Bordeaux, France, Chaussade et al (2022) reported HPV incidence rate ratios (IRR) of 1.8 [1.4–2.2] in a cohort of HIV-positive patients with lung cancer (incidence rates for 100 000 people per year) [ 108 ].…”

Section: Epidemiology Of Hpv In Lung Cancermentioning

confidence: 99%

High-Risk Human Papillomavirus Infection in Lung Cancer: Mechanisms and Perspectives

Osorio

Candia-Escobar

Corvalán

et al. 2022

Biology

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Lung cancer is a very prevalent and heterogeneous group of malignancies, and most of them are etiologically associated with tobacco smoking. However, viral infections have been detected in lung carcinomas, with high-risk human papillomaviruses (HR-HPVs) being among them. The role of HR-HPVs in lung cancer has been considered to be controversial. This issue is due to the highly variable presence of this virus in lung carcinomas worldwide, and the low viral load frequently that is detected. In this review, we address the epidemiological and mechanistic findings regarding the role of HR-HPVs in lung cancer. Some mechanisms of HR-HPV-mediated lung carcinogenesis have been proposed, including (i) HPV works as an independent carcinogen in non-smoker subjects; (ii) HPV cooperates with carcinogenic compounds present in tobacco smoke; (iii) HPV promotes initial alterations being after cleared by the immune system through a “hit and run” mechanism. Additional research is warranted to clarify the role of HPV in lung cancer.

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The absence of high-risk human papillomavirus in Czech non-small cell lung cancer cases

Cited by 7 publications

References 21 publications

Infection with human cytomegalovirus, Epstein-Barr virus, and high-risk types 16 and 18 of human papillomavirus in EGFR-mutated lung adenocarcinoma

Infection with human cytomegalovirus, Epstein-Barr virus, and high-risk types 16 and 18 of human papillomavirus in EGFR-mutated lung adenocarcinoma

Pitfalls of commercially available HPV tests in HPV68a detection

High-Risk Human Papillomavirus Infection in Lung Cancer: Mechanisms and Perspectives

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