The absence of direct antimicrobial activity in extracts of cytotoxic lymphocytes

Joag, Sanjay V.; Young, John Ding‐E

doi:10.1016/0165-2478(89)90190-9

Cited by 3 publications

(1 citation statement)

References 11 publications

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“…Bactericidal activities of T cells were examined according to the method described by Joag and Young (10). Separated splenic T cells were put onto 96 well plates by 1´10…”

Section: Bactericidal Activity Of Splenic T Cellsmentioning

confidence: 99%

Addition of Maitake D-fraction Reduces the Effective Dosage of Vancomycin for the Treatment of Listeria-Infected Mice

Kodama

Yamada

Nanba

2001

Japanese Journal of Pharmacology

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ABSTRACT-Maitake D-fraction, b1,6-glucan having b1,3-branches, has been reported to activate the immune system of the host. To elucidate whether the D-fraction can reduce the clinical effective dosage of antibiotics in the treatment of opportunistic bacterial infection, we examined the effects of D-fraction on the treatment of Listeria monocytogenes-infected mice in combination with vancomycine (VCM), the only antibiotic used for methicillin-resistant Staphylococcus aureus (MRSA). Listeria-infection was introduced by its inoculation into the abdominal cavity of mice. Without treatment, all inoculated mice died within 3 days after the inoculation. In contrast, in the mice treated with combined therapy of D-faction (10 mg /kg per day) and VCM (10 mg /kg per day), the survival rate was maintained at 60% on the 10th day after the inoculation, which was superior to that of mice treated with VCM alone (10 mg /kg per day). To investigate the mechanism underlying the reinforcement of VCM treatment by the D-fraction, the activities of macrophages and splenic T cells of Listeria-infected mice were evaluated. In mice administered with both D-fraction and VCM, macrophages produced 2.7 times as much interleukin-1 as that of non-treated control mice. The bactericidal activity of splenic T cells was also enhanced by 2.6 times of that of non-treated control mice. These results indicate that D-fraction activates immuno-competent cells, induced cytokine production, and consequently enhanced the bactericidal activities of the splenic T cells against Listeria monocytogenes, suggesting the clinical benefit of D-fraction in the case of anti-bacterial treatment for patients with high risks.

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“…Bactericidal activities of T cells were examined according to the method described by Joag and Young (10). Separated splenic T cells were put onto 96 well plates by 1´10…”

Section: Bactericidal Activity Of Splenic T Cellsmentioning

confidence: 99%

Addition of Maitake D-fraction Reduces the Effective Dosage of Vancomycin for the Treatment of Listeria-Infected Mice

Kodama

Yamada

Nanba

2001

Japanese Journal of Pharmacology

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Overview of CTL-Target Adhesion and Other Critical Events in the Cytotoxic Mechanism

Martz¹

1993

Cytotoxic Cells: Recognition, Effector Function, Generation, and Methods

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Can CTL Control Virus Infections Without Cytolysis? The Prelytic Halt Hypothesis

Martz¹

1993

Cytotoxic Cells: Recognition, Effector Function, Generation, and Methods

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The absence of direct antimicrobial activity in extracts of cytotoxic lymphocytes

Cited by 3 publications

References 11 publications

Addition of Maitake D-fraction Reduces the Effective Dosage of Vancomycin for the Treatment of Listeria-Infected Mice

Addition of Maitake D-fraction Reduces the Effective Dosage of Vancomycin for the Treatment of Listeria-Infected Mice

Overview of CTL-Target Adhesion and Other Critical Events in the Cytotoxic Mechanism

Can CTL Control Virus Infections Without Cytolysis? The Prelytic Halt Hypothesis

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