The ability of two different Vibrio spp. bacteriophages to infect Vibrio harveyi, Vibrio cholerae and Vibrio mimicus

Payne, Mark; Oakey, Jane; Owens, Leigh

doi:10.1111/j.1365-2672.2004.02362.x

Cited by 17 publications

(8 citation statements)

References 25 publications

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“…The determination of the complete DNA sequence of the prototype phage VP58.5 revealed very strong similarity to phage VHML. Besides V. harveyi strains, VHML has been reported to lyse the strains Vibrio alginolyticus ACMM102 and Vibrio cholerae ATCC 14035, but there are no published data on the infectiousness for V. parahaemolyticus (33). On the other hand, we could not find a V. harveyi strain susceptible to VP58.5.…”

Section: Discussioncontrasting

confidence: 49%

The Linear Plasmid Prophage Vp58.5 of Vibrio parahaemolyticus Is Closely Related to the Integrating Phage VHML and Constitutes a New Incompatibility Group of Telomere Phages

Zabala

Hammerl

Espejo

et al. 2009

J Virol

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Vibrio parahaemolyticus O3:K6 pandemic strains recovered in Chile frequently possess a 42-kb plasmid which is the prophage of a myovirus. We studied the prototype phage VP58.5 and show that it does not integrate into the host cell chromosome but replicates as a linear plasmid (Vp58.5) with covalently closed ends (telomeres). The Vp58.5 replicon coexists with other plasmid prophages (N15, PY54, and ⌽KO2) in the same cell and thus belongs to a new incompatibility group of telomere phages. We determined the complete nucleotide sequence (42,612 nucleotides) of the VP58.5 phage DNA and compared it with that of the plasmid prophage. The two molecules share the same nucleotide sequence but are 35% circularly permuted to each other. In contrast to the hairpin ends of the plasmid, VP58.5 phage DNA contains 5-protruding ends. The VP58.5 sequence is 92% identical to the sequence of phage VHML, which was reported to integrate into the host chromosome. However, the gene order and termini of the phage DNAs are different. The VHML genome exhibits the same gene order as does the Vp58.5 plasmid. VHML phage DNA has been reported to contain terminal inverted repeats. This repetitive sequence is similar to the telomere resolution site (telRL) of VP58.5 which, after processing by the phage protelomerase, forms the hairpin ends of the Vp58.5 prophage. It is discussed why these closely related phages may be so different in terms of their genome ends and their lifestyle.Most temperate bacteriophages integrate into the host chromosome during lysogeny. However, there are some phages (telomere phages) whose prophages are linear plasmids with covalently closed ends. Members of this group of phages are the siphoviruses N15, PY54, and ⌽KO2 isolated from Escherichia coli, Yersinia enterocolitica, and Klebsiella oxytoca, respectively, and the recently described myoviruses ⌽HAP-1 of Halomonas aquamarina and VP882 of Vibrio parahaemolyticus (6,20,23,26,37). Despite their different origins (enterobacteria versus marine bacterium) and morphologies, all known telomere phages share similar genome organizations and some protein similarities. The linear DNA of each phage is a circular permutation of the respective linear plasmid prophage. For the generation of the terminal hairpins of the linear plasmid, the protelomerase (Tel) is essential (8). This enzyme has cleaving/joining activity; its target is a large palindromic DNA sequence called the telomere resolution site (telRL) located upstream of tel on the phage genome. After cleaving telRL by staggered cuts, the resulting self-complementary single-stranded DNA overhangs fold back and are rejoined by the protelomerase (9). Besides tel, all telomere phages possess the gene repA, encoding a multifunctional replication protein. repA of N15 and PY54 was shown to harbor the prophage replication origin and to function as a circular minimal replicon (35,42). Compatibility studies demonstrated that the N15 and ⌽KO2 plasmids belong to the same incompatibility group, whereas the PY54 plasmid is able to coexist...

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Section: Discussioncontrasting

confidence: 49%

The Linear Plasmid Prophage Vp58.5 of Vibrio parahaemolyticus Is Closely Related to the Integrating Phage VHML and Constitutes a New Incompatibility Group of Telomere Phages

Zabala

Hammerl

Espejo

et al. 2009

J Virol

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“…Oakey and Owens found that VHML has a narrow range and only infected four of the 36 strains tested. However, some other species of Vibrio, notably V. cholerae (ACM #2773/ATCC #14035) outside the Harveyi clade [19] and V. alginolyticus [16] could be infected by VHML. Of note was that the strains of the Harveyi clade successfully infected were mostly different between the two bacteriophages.…”

Section: Discussionmentioning

confidence: 99%

Virulence Changes to Harveyi Clade Bacteria Infected with Bacteriophage from Vibrio owensii

Busico-Salcedo

Owens

2013

Indian J. Virol.

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Vibrio owensii is one of the most virulent vibrios known being able to kill crustacean larvae at 10 2 CFU ml -1 . This study describes virulence changes to naïve strains of Vibrio harveyi and Vibrio campbellii when infected with the bacteriophage VOB from a closely related species V. owensii 47666-1. The bacteriophage from V. owensii was induced into lytic phase by using mitomycin C at 100 ng ml -1 . One strain of V. harveyi and two strains of V. campbellii from 29 tested containing no prophage were susceptible to lysogenic conversion with VOB. Virulence changes induced in Harveyi clade bacteria included the upregulation of protein secretion, statistically significant increased haemolysin and chitinase production and increased mortality to nauplii of Penaeus monodon. No change in siderophore production was observed. Bacteriophage VOB is likely to be responsible for some of the virulence factors expressed by V. owensii. As this bacteriophage is able to infect strains of V. harveyi and V. campbellii this phage may contribute to increased virulence of other vibrios in aquaculture and in the natural environment.

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“…Similarly, V. harveyi myovirus like (VHML) particles are reported to be associated with virulence in V. harveyi (Munro et al, 2003). Nine (5 isolates recovered from infected larvae and 4 from water samples) of V. harveyi isolates obtained during the LB disease outbreak in the present study were screened for VHML by polymerase chain reaction (PCR) (Payne et al, 2004) (data not given) and none of these isolates produced the 900 bp product specific for tail sheeth encoding gene of VHML (instead, a 350 bp fragment could be amplified). Occurrence of luminous vibriosis in larval shrimp by LB isolates that lack VHML suggests possibilities of involvement of other bacteriophages similar to VHML in the virulence of V. harveyi.…”

Section: Discussionmentioning

confidence: 99%

Distribution of luminescent Vibrio harveyi and their bacteriophages in a commercial shrimp hatchery in South India

et al. 2008

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Luminescent Vibrio harveyi is a natural microflora of marine and coastal water bodies and is associated with mortality of larval shrimp in penaeid shrimp hatcheries. It is also known that the bacteriophages occur virtually in all places where their hosts exist. In this study, distribution of luminescent V. harveyi and the bacteriophages affecting these hosts was examined in a commercial Penaeus monodon hatchery during three shrimp larval production cycles, including a cycle affected by luminescent bacterial (LB) disease outbreak.Out of a total of 1195 samples drawn from seawater source, sand-filtered water, nauplius, zoea, mysis and post larval rearing tanks, maturation and spawning tanks, Artemia hatching tank and algal culture tanks processed using conventional microbiological techniques, 21.4% of the samples harboured luminescent bacteria. During the larval production cycle affected by LB disease (LBD), luminescent V. harveyi could be recovered from 52% of the hatchery samples, whereas during luminescent bacterial disease-free larval production cycle (LBDF), these bacteria could be recovered from only about 9% of the samples. The predominant source of luminescent bacteria was the brood shrimp and their rearing tanks in maturation and spawning facilities. 73% of the maturation and 80% of the spawning tank water samples harbored LB during LBD, whereas, only 20% and 32% of the maturation and spawning tanks respectively harbored LB during LBDF. LB could be isolated from 17% of the water samples in tanks from nauplius stage onwards with increasing counts that subsequently lead to LB disease.Bacteriophages affecting the luminescent V. harveyi could be isolated from as many as 36% (21% and 43% of the samples analysed during LBDF and LBD respectively) of a total of 181 water samples drawn from various sources in the hatchery, using 27 luminescent V. harveyi hosts by agar overlay technique. The maturation tank water samples were found to be the predominant source of bacteriophages, followed by spawning tank water samples as observed with the LB. Sixty five bacteriophages, 13 during LBDF and 52 during LBD were isolated, which were grouped in to seven types based on their plaque morphology.The study has indicated that the brooders, maturation and spawning facilities in the shrimp hatchery are the main source of luminescent V. harveyi and their bacteriophages and that occurrence of LB even in low counts during early larval stages can possibly lead to development of LB disease despite presence of bacteriophages in the larval rearing tanks.

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The ability of two different Vibrio spp. bacteriophages to infect Vibrio harveyi, Vibrio cholerae and Vibrio mimicus

Cited by 17 publications

References 25 publications

The Linear Plasmid Prophage Vp58.5 of Vibrio parahaemolyticus Is Closely Related to the Integrating Phage VHML and Constitutes a New Incompatibility Group of Telomere Phages

The Linear Plasmid Prophage Vp58.5 of Vibrio parahaemolyticus Is Closely Related to the Integrating Phage VHML and Constitutes a New Incompatibility Group of Telomere Phages

Virulence Changes to Harveyi Clade Bacteria Infected with Bacteriophage from Vibrio owensii

Distribution of luminescent Vibrio harveyi and their bacteriophages in a commercial shrimp hatchery in South India

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