The ability of sulfate-reducing bacteria Desulfovibrio desulfuricans Ya-11 i Desulfobacter sp. to use nitrate as electron acceptor

Peretyatko, T. B.; Gudz, S. P.

doi:10.30970/sbi.0502.150

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…The ability of bacteria to use different electron acceptors such as sulfate, sulfite, thiosulfate, fumarate in the amount of 4.5 g/L (Asaulenko et al, 2010) and nitrate 3 and at a concentration of 10 μM) (Peretyatko & Gudz, 2011) was investigated in Postgate's "C" medium. Ferum (II) sulfate was replaced by ferric (II) chloride.…”

Section: Physiological-biochemical Properties Of the Isolated Strainsmentioning

confidence: 99%

Genotypic, physiological and biochemical features of Desulfovibrio strains in a sulfidogenic microbial community isolated from the soil of ferrosphere

Tkachuk¹,

Zelena

Mazur

et al. 2020

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The purpose of this work was the isolation of the predominant representatives of sulfate-reducing bacteria (SRB) of the sulfidogenic microbial community separated from the soil ferrosphere and the examination of their morphological, physiological, biochemical and genotypic peculiarities, the evaluation of some physiological processes under co-culturing with their satellite species Anaerotignum propionicum. During the study two isolates of sulfate-reducing bacteria NUChC SRB1 and NUChC SRB2 were obtained from sulfidogenic microbial community isolated from soil ferrosphere on Postgate's "B" medium and their belonging to different strains (using ISSR-PCR method) was proved. As a result of molecular-genetic analysis of the strains, a 16S rRNA gene fragments of 613 bp and 522 bp were amplified and sequenced. The strains were identified as Desulfovibrio oryzae by the complex of microbiological, physiological and biochemical features and on the basis of 16S rRNA gene sequences (phylogenetic analysis). The 16S rRNA gene sequences were submitted in GenBank as MT102713 (NUChC SRB1) and MT102714 (NUChC SRB2). The co-cultivation of isolated SRB strains with A. propionicum NUChC Sat1 strain (in the absence of electron donors, the presence of sulfates and yeast extract) showed the formation of sulfur-reducing bacteria of hydrogen sulfide, which was not observed during their mono-cultivation. In this case, the phenomenon of syntrophy probably takes place-co-growth on the nutrient substrate, and the electron donor appears due to the use of the yeast extract compounds by the NUChC Sat1 strain. Therefore, in the sulfidogenic community isolated from the soil ferrosphere, there is a mutual growth of the association of bacteria D. oryzae and A. propionicum, which is caused by trophic interaction. Possibly the contribution of these associated bacteria to the corrosion process lies in the utilization of hydrogen (D. oryzae) and the formation of substrate products of SRB metabolism (hydrogen and organic acids), which are both corrosive compounds (A. propionicum). Without a doubt the corrosion process involving this association needs further investigation.ferrosphere, sulfate-reducing bacteria, Desulfovibrio oryzae, 16S rRNA gene, ISSR-PCR.

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Section: Physiological-biochemical Properties Of the Isolated Strainsmentioning

confidence: 99%

Genotypic, physiological and biochemical features of Desulfovibrio strains in a sulfidogenic microbial community isolated from the soil of ferrosphere

Tkachuk¹,

Zelena

Mazur

et al. 2020

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“…Nitrate reductase NarGHI is an enzymatic complex that includes multi-heme b-type cytochrome, proteins with Fe-S clusters, and Mo-containing cofactor [13,21]. Nitrate reduction with the formation of nitrite and its further reduction by complex of periplasmic dissimilatory nitrite reductases to NH 4 + was descri bed in Desulfovibrio desulfuricans, Desulfotomaculum sp., Desulfobacter sp., and Wolinella succinogenes [4,11,20,25,29,39].…”

Section: Introductionmentioning

confidence: 99%

Reduction of sulfate, nitrate and nitrite ions by Desulfovibrio sp. under the influence of ferrum (III) citrate

2020

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Dissimilatory reduction of sulfate, nitrate and nitrite ions by bacteria Desulfovibrio sp. under the influence of potassium dichromate

Moroz

Hnatush

Yavorska

et al. 2022

Regul. Mech. Biosyst.

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In the process of anaerobic respiration, sulfate reducing bacteria, besides sulfates, can use other electron acceptors: nitrates, nitrites, oxidized forms of heavy metals, in particular, hexavalent chromium, which are harmful for organisms. Selection of pollutant-resistant stains of this kind of bacteria isolated from technogenically altered ecotopes, capable of reductive transformation of various nature pollutants, is an especially relevant task for the creation of new effective remediation biotechnologies. The purpose of this work was to investigate the regularities of usage of sulfate, nitrate or nitrite ions by bacteria of the Desulfovibrio genus, isolated from Yavorivske Lake, at conditions of simultaneous presence in the medium of another electron acceptor – Cr(VI), to establish a succession of electron acceptors’ reduction by investigated sulfidogenic bacteria and to evaluate the efficiency of their possible application in technologies of complex purification of the environment from metal, sulfur and nitrogen compounds. Bacteria were grown under anaerobic conditions for 10 days in Kravtsov-Sorokin medium without Mohr’s salt. To study the efficiency of sulfate, nitrate, or nitrite ions’ reduction at simultaneous presence in the medium of Cr(VI), bacteria were sown in media with Na2SO4×10H2O, NaNO3, NaNO2 or K2Cr2O7 to final SO42–, NO3–, NO2– or Cr(VI) concentration in the medium of 3.47 (concentration of SO42– in medium of standard composition) or 1.74, 3.47, 5.21, 6.94, 10.41 mM. Biomass was determined turbidimetrically, and the concentrations of sulfate, nitrate, nitrite, ammonium ions, hydrogen sulfide, Cr(VI), Cr(ІІІ) in cultural liquid were determined by spectrophotometric method. It has been established that Cr(VI) inhibits the biomass accumulation, sulfate ions’ reduction and hydrogen sulfide production by Desulfovibrio sp. after simultaneous introduction into the medium of 3.47 mM SO42– and 1.74–10.41 mM Cr(VI). In the medium with the same initial content (3.47 mM) of SO42– and Cr(VI), bacteria reduced 2.1–2.3 times more Cr(VI) than sulfate ions with Cr(III) production at concentrations up to 2.2 times higher than hydrogen sulfide. It has been shown that K2Cr2O7 inhibits the biomass accumulation, the nitrate ions reduction and the ammonium ions production by bacteria after simultaneous addition into the medium of 3.47 mM NO3– and 1.74–10.41 mM Cr(VI) or 1.74–10.41 mM NO3– and 3.47 mM Cr(VI). In the medium with the same initial content (3.47 mM) of NO3– and Cr(VI) bacteria reduced 1.1–1.3 times more nitrate ions than Cr(VI) with the production of ammonium ions at concentrations up to 1.3 times higher than that of Cr(III). It has been established that K2Cr2O7 inhibits the biomass accumulation, the nitrite ions’ reduction and the ammonium ions’ production by bacteria after simultaneous addition into the medium of 3.47 mM NO2– and 1.74–10.41 mM Cr(VI) or 1.74–10.41 mM NO2– and 3.47 mM Cr(VI). In the medium with the same initial content (3.47 mM) NO2– and Cr(VI) the reduction of Cr(VI) by bacteria practically did not differ from the reduction of nitrite ions (was only slightly lower – up to 1.1 times), almost the same concentrations of trivalent chromium and ammonium ions in the cultural liquid were detected. The processes of nitrate and nitride reduction, carried out by bacteria of Desulfovibrio genus, were revealed to be less sensitive to the negative influence of sodium dichromate, as compared with the process of sulfate ions’ reduction, which in the medium with 3.47 mM SO42– and 1.74–10.41 mM Cr(VІ) decreased by 3.2–4.6 times as compared with this process in the medium with only Na2SO4×10H2O. The investigated strains of bacteria are adapted to high concentrations of toxic pollutants (up to 10.41 mM) and therefore are promising for application in technologies of complex environment purification from hexavalent chromium, sulfur and nitrogen compounds.

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The ability of sulfate-reducing bacteria Desulfovibrio desulfuricans Ya-11 i Desulfobacter sp. to use nitrate as electron acceptor

Cited by 3 publications

References 10 publications

Genotypic, physiological and biochemical features of Desulfovibrio strains in a sulfidogenic microbial community isolated from the soil of ferrosphere

Genotypic, physiological and biochemical features of Desulfovibrio strains in a sulfidogenic microbial community isolated from the soil of ferrosphere

Reduction of sulfate, nitrate and nitrite ions by Desulfovibrio sp. under the influence of ferrum (III) citrate

Dissimilatory reduction of sulfate, nitrate and nitrite ions by bacteria Desulfovibrio sp. under the influence of potassium dichromate

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