The ability of farnesol to prevent adhesion and disrupt Fusarium keratoplasticum biofilm

Kischkel, Brenda; Souza, Gustavo Henrique de; Chiavelli, Lucas Ulisses Rovigatti; Pomini, Armando M.; Svidzinski, Terezinha Inez Estivalet; Negri, Melyssa

doi:10.1007/s00253-019-10233-2

Cited by 27 publications

(14 citation statements)

References 47 publications

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“…A. versicolor is very frequent in natural environments on different organic substrates; it often releases a high number of spores in air but is clinically not relevant.Within the genus Fusarium, there are many important plant pathogens with broad host specificity and strong saprophytic properties.F. proliferatum)(Al-Hatmi et al, 2016;Al-Maqtoofi & Thornton, 2016;Kischkel et al, 2020;Short et al, 2011). Among the over 40 phylogenetically distinct FSSC(Zhang et al 2006), there are mainly four clinically important Neocosmospora taxa, N. petroliphila, N. keratoplastica, N. falciformis, and N. metavorans, causing different forms of superficial infections (e.g., keratitis and eye infections) but occasionally also invasive fusariosis (Table3)(Sandoval-Denis & Crous, 2018).…”

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confidence: 99%

“…. proliferatum)(Al-Hatmi et al, 2016;Al-Maqtoofi & Thornton, 2016;Kischkel et al, 2020;Short et al, 2011). Among the over 40 phylogenetically distinct FSSC(Zhang et al 2006), there are mainly four clinically important Neocosmospora taxa, N. petroliphila, N. keratoplastica, N. falciformis, and N. metavorans, causing different forms of superficial infections (e.g., keratitis and eye infections) but occasionally also invasive fusariosis (Table3)(Sandoval-Denis & Crous, 2018).…”

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confidence: 99%

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Crossover between the control of fungal pathogens in medicine and the wider environment, and the threat of antifungal resistance

Gisi¹

2021

Plant Pathology

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A primary human pathogen is an agent (fungus, bacterium, virus) causing any kind of disease in humans, either superficially or within the body (invasive, systemic). All fungal propagules (all kind of cells, spores) existing in the natural environment can be transmitted to the human body. Fungal infections usually start in the lung by inhalation of contaminated air, or on the skin, nails, and mucosa (vagina, mouth, sinuses) by direct contact (e.g., through wounds), and rarely also in the gastrointestinal tract by intake of contaminated food or water. Fungal infections in humans are rarely serious (lifethreatening) unless the patient's immune system is weakened, for

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Crossover between the control of fungal pathogens in medicine and the wider environment, and the threat of antifungal resistance

Gisi¹

2021

Plant Pathology

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“…JawsII cells (ATCC ® CRL-11904™, immature dendritic cell; monocyte - bone marrow from C57BL/6) were used. Untreated controls, or wells containing only the peptide diluents or cell culture medium were used ( 28 ). Peptides (HcP 1-4) were evaluated at a concentration of 10 µg per well, GP-HcP 1-4 and GP-HcP 2,3,4; 2,3 or 1,4 were tested at 5 µg per well.…”

Section: Methodsmentioning

confidence: 99%

Immunoproteomic and Immunopeptidomic Analyses of Histoplasma capsulatum Reveal Promiscuous and Conserved Epitopes Among Fungi With Vaccine Potential

et al. 2021

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As there are more than 6 million human deaths due to mycoses each year, there is an urgent need to develop fungal vaccines. Moreover, given the similarities among pathogenic fungi, it may be possible to create a multi-fungi vaccine. In this study, we combined immunoproteomic and immunopeptidomic methods, for which we have adapted a technique based on co-immunoprecipitation (Co-IP) that made it possible to map Histoplasma capsulatum epitopes for the first time in a natural context using murine dendritic cells (DCs) and macrophages (Mφ). Although polysaccharide epitopes exist, this research focused on mapping protein epitopes as these are more immunogenic. We used different algorithms to screen proteins and peptides identified by two-dimensional electrophoresis (2-D) and Co-IP. Seventeen proteins were revealed by 2-D gels, and 45 and 24 peptides from distinct proteins were presented by DCs and Mφ, respectively. We then determined which epitopes were restricted to MHC-I and II from humans and mice and showed high promiscuity, but lacked identity with human proteins. The 4 most promising peptides were synthesized, and the peptides with and without incorporation into glucan particles induced CD4+ and CD8+ T cell proliferation and produced a Th1 and Th17 response marked by the secretion of high levels of IFN-γ, IL-17 and IL-2. These epitopes were from heat shock protein 60, enolase, and the ATP-dependent molecular chaperone HSC82, and they each have a high degree of identity with proteins expressed by other medically important pathogenic fungi. Thus, the epitopes described in this study have the potential for use in the development of vaccines that could result in cross-protection among fungal species.

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“…At 125 µg/mL, vanillin protected C. elegans against C. albicans infection and enhanced its survival [ 155 ]. Kischkel et al [ 156 ] evaluated farnesol on preformed F. solani complex biofilms particularly formed by Fusarium keratoplasticum, which is the most prevalent fungi related to biofilm formation in hospital water systems and in internal pipelines. Farnesol showed activity against F. keratoplasticum preformed biofilms, and was effective also during its formation at different times (at adhesion and at 24, 48 and 72 h) with a complete inhibition at 700 µM, as assessed by counting the CFU number and by CV and XTT assays.…”

Section: Reported Antibiofilm Activities Of Pure Natural Compoundsmentioning

confidence: 99%

Fungal Biofilms as a Valuable Target for the Discovery of Natural Products That Cope with the Resistance of Medically Important Fungi—Latest Findings

et al. 2021

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The development of new antifungal agents that target biofilms is an urgent need. Natural products, mainly from the plant kingdom, represent an invaluable source of these entities. The present review provides an update (2017–May 2021) on the available information on essential oils, propolis, extracts from plants, algae, lichens and microorganisms, compounds from different natural sources and nanosystems containing natural products with the capacity to in vitro or in vivo modulate fungal biofilms. The search yielded 42 articles; seven involved essential oils, two Brazilian propolis, six plant extracts and one of each, extracts from lichens and algae/cyanobacteria. Twenty articles deal with the antibiofilm effect of pure natural compounds, with 10 of them including studies of the mechanism of action and five dealing with natural compounds included in nanosystems. Thirty-seven manuscripts evaluated Candida spp. biofilms and two tested Fusarium and Cryptococcus spp. Only one manuscript involved Aspergillus fumigatus. From the data presented here, it is clear that the search of natural products with activity against fungal biofilms has been a highly active area of research in recent years. However, it also reveals the necessity of deepening the studies by (i) evaluating the effect of natural products on biofilms formed by the newly emerged and worrisome health-care associated fungi, C. auris, as well as on other non-albicans Candida spp., Cryptococcus sp. and filamentous fungi; (ii) elucidating the mechanisms of action of the most active natural products; (iii) increasing the in vivo testing.

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The ability of farnesol to prevent adhesion and disrupt Fusarium keratoplasticum biofilm

Cited by 27 publications

References 47 publications

Crossover between the control of fungal pathogens in medicine and the wider environment, and the threat of antifungal resistance

Crossover between the control of fungal pathogens in medicine and the wider environment, and the threat of antifungal resistance

Immunoproteomic and Immunopeptidomic Analyses of Histoplasma capsulatum Reveal Promiscuous and Conserved Epitopes Among Fungi With Vaccine Potential

Fungal Biofilms as a Valuable Target for the Discovery of Natural Products That Cope with the Resistance of Medically Important Fungi—Latest Findings

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