The AAA+ motor complex of subunits CobS and CobT of cobaltochelatase visualized by single particle electron microscopy

Lundqvist, Joakim; Elmlund, Dominika; Heldt, Dana; Deery, Evelyne; Söderberg, Christopher; Hansson, Mats; Warren, Martin J.; Al-Karadaghi, Salam

doi:10.1016/j.jsb.2009.06.013

Cited by 38 publications

(25 citation statements)

References 37 publications

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“…The Mg-chelatase complex has both sequence and structural homology with the Class I cobalt chelatase of the O 2 -dependent cobalamin biosynthetic pathway (Lundqvist et al 2009). The CobN, CobS, and CobT subunits of the trimeric cobalt chelatase are homologous with the BchH/ChlH, BchI/ChlI, and BchD/ChlD subunits of magnesium chelatase, respectively (Fodje et al 2001; Lundqvist et al 2009).…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…The CobN, CobS, and CobT subunits of the trimeric cobalt chelatase are homologous with the BchH/ChlH, BchI/ChlI, and BchD/ChlD subunits of magnesium chelatase, respectively (Fodje et al 2001; Lundqvist et al 2009). This homology has been interpreted as reflecting ancient duplication and divergence (Lundqvist et al 2009). Although there is a broad taxonomic distribution of the CobN gene among cobalamin-dependent organisms, a much narrower distribution is observed for the genes that compose the AAA+ motor complex ( CobS and CobT ) (Rodionov et al 2003).…”

Section: Resultsmentioning

confidence: 99%

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Chlorophyll Biosynthesis Gene Evolution Indicates Photosystem Gene Duplication, Not Photosystem Merger, at the Origin of Oxygenic Photosynthesis

Sousa

Shavit-Grievink

Allen

et al. 2012

Genome Biology and Evolution

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An open question regarding the evolution of photosynthesis is how cyanobacteria came to possess the two reaction center (RC) types, Type I reaction center (RCI) and Type II reaction center (RCII). The two main competing theories in the foreground of current thinking on this issue are that either 1) RCI and RCII are related via lineage divergence among anoxygenic photosynthetic bacteria and became merged in cyanobacteria via an event of large-scale lateral gene transfer (also called "fusion" theories) or 2) the two RC types are related via gene duplication in an ancestral, anoxygenic but protocyanobacterial phototroph that possessed both RC types before making the transition to using water as an electron donor. To distinguish between these possibilities, we studied the evolution of the core (bacterio)chlorophyll biosynthetic pathway from protoporphyrin IX (Proto IX) up to (bacterio)chlorophyllide a. The results show no dichotomy of chlorophyll biosynthesis genes into RCI- and RCII-specific chlorophyll biosynthetic clades, thereby excluding models of fusion at the origin of cyanobacteria and supporting the selective-loss hypothesis. By considering the cofactor demands of the pathway and the source genes from which several steps in chlorophyll biosynthesis are derived, we infer that the cell that first synthesized chlorophyll was a cobalamin-dependent, heme-synthesizing, diazotrophic anaerobe.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Chlorophyll Biosynthesis Gene Evolution Indicates Photosystem Gene Duplication, Not Photosystem Merger, at the Origin of Oxygenic Photosynthesis

Sousa

Shavit-Grievink

Allen

et al. 2012

Genome Biology and Evolution

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“…given that CobS and CobT have a common evolutionary origin with, respectively, the magnesium chelatase subunits BchI and BchD 19 , and considering that BchI is known to be a chaperone essential for the function of the BchD magnesium chelatase subunit 20 Supplementary Fig. 3).…”

Section: Biosynthesis Of Cbad In E Colimentioning

confidence: 99%

Metabolic engineering of Escherichia coli for de novo biosynthesis of vitamin B₁₂

Fang

Liu

Kang

et al. 2018

Preprint

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The only known source of vitamin B12 (adenosylcobalamin) is from bacteria and archaea, and the only unknown step in its biosynthesis is the production of the intermediate adenosylcobinamide phosphate. Here, using genetic and metabolic engineering, we generated an Escherichia coli strain that produces vitamin B12 via an engineered de novo aerobic biosynthetic pathway. Excitingly, the BluE and CobC enzymes from Rhodobacter capsulatus transform L-threonine into (R)-1-Amino-2-propanol O-2-Phosphate, which is then condensed with adenosylcobyric acid to yield adenosylcobinamide phosphate by either CobD from the aeroic R. capsulatus or CbiB from the anerobic Salmonella typhimurium. These findings suggest that the biosynthetic steps from co(II)byrinic acid a,c-diamide to adocobalamin are the same in both the aerobic and anaerobic pathways. Finally, we increased the vitamin B12 yield of a recombinant E. coli strain by more than ~250-fold to 307.00 µg/g DCW via metabolic engineering and optimization of fermentation conditions. Beyond our scientific insights about the aerobic and anaerobic pathways and our demonstration of E. coli as a microbial biosynthetic platform for vitamin B12 production, our study offers an encouraging example of how the several dozen proteins of a complex biosynthetic pathway can be transferred between organisms to facilitate industrial production.

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“…Only one of probably six intermediates has been identified [108], and the enzymes involved are not yet known. Two chelatases structurally related to the cobalt chelatase CobNS [109] could be nickel chelatases that catalyze the incorporation of Ni 2+ into precorrin-2 or a precorrin-2 product (MTBMA_c10550-10570, 09440; MTH673, 556). It has been proposed that proteins structurally related to the nitrogenase [Fe]-protein NifH and the [MoFe]-protein NifDK could have a function in pyrrole ring reduction involved in the synthesis of F 430 from precorrin-2 [110].…”

Section: Genes For the Synthesis Of Prosthetic Groups Of Methanogmentioning

confidence: 99%

More Than 200 Genes Required for Methane Formation from H₂and CO₂and Energy Conservation Are Present inMethanothermobacter marburgensisandMethanothermobacter thermautotrophicus

Kaster

Goenrich

Seedorf

et al. 2011

Archaea

109

115

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The hydrogenotrophic methanogens Methanothermobacter marburgensis and Methanothermobacter thermautotrophicus can easily be mass cultured. They have therefore been used almost exclusively to study the biochemistry of methanogenesis from H2 and CO2, and the genomes of these two model organisms have been sequenced. The close relationship of the two organisms is reflected in their genomic architecture and coding potential. Within the 1,607 protein coding sequences (CDS) in common, we identified approximately 200 CDS required for the synthesis of the enzymes, coenzymes, and prosthetic groups involved in CO2 reduction to methane and in coupling this process with the phosphorylation of ADP. Approximately 20 additional genes, such as those for the biosynthesis of F430 and methanofuran and for the posttranslational modifications of the two methyl-coenzyme M reductases, remain to be identified.

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The AAA+ motor complex of subunits CobS and CobT of cobaltochelatase visualized by single particle electron microscopy

Cited by 38 publications

References 37 publications

Chlorophyll Biosynthesis Gene Evolution Indicates Photosystem Gene Duplication, Not Photosystem Merger, at the Origin of Oxygenic Photosynthesis

Chlorophyll Biosynthesis Gene Evolution Indicates Photosystem Gene Duplication, Not Photosystem Merger, at the Origin of Oxygenic Photosynthesis

Metabolic engineering of Escherichia coli for de novo biosynthesis of vitamin B₁₂

More Than 200 Genes Required for Methane Formation from H₂and CO₂and Energy Conservation Are Present inMethanothermobacter marburgensisandMethanothermobacter thermautotrophicus

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The AAA+ motor complex of subunits CobS and CobT of cobaltochelatase visualized by single particle electron microscopy

Cited by 38 publications

References 37 publications

Chlorophyll Biosynthesis Gene Evolution Indicates Photosystem Gene Duplication, Not Photosystem Merger, at the Origin of Oxygenic Photosynthesis

Chlorophyll Biosynthesis Gene Evolution Indicates Photosystem Gene Duplication, Not Photosystem Merger, at the Origin of Oxygenic Photosynthesis

Metabolic engineering of Escherichia coli for de novo biosynthesis of vitamin B12

More Than 200 Genes Required for Methane Formation from H2and CO2and Energy Conservation Are Present inMethanothermobacter marburgensisandMethanothermobacter thermautotrophicus

Contact Info

Product

Resources

About

Metabolic engineering of Escherichia coli for de novo biosynthesis of vitamin B₁₂

More Than 200 Genes Required for Methane Formation from H₂and CO₂and Energy Conservation Are Present inMethanothermobacter marburgensisandMethanothermobacter thermautotrophicus