The A and the extended V N‐terminal regions of streptococcal protein I/IIf mediate the production of tumour necrosis factor α in the monocyte cell line THP‐1

BAFF synthesis by rheumatoid synoviocytes is positively controlled by α5β1 integrin stimulation and is negatively regulated by tumor necrosis factor α and toll‐like receptor ligands

“…Synthetic BLP, palmitoyl-3-cysteine-serine-lysine-4 (Pam 3 CSK 4 ), was obtained from EMC Microcollections (Tübingen, Germany). Streptococcal protein I/II was prepared as previously described (20). Potential endotoxins in the protein I/II preparation were removed using polymyxin B-agarose (Pierce, Rockford, IL) according to the manufacturer's recommendations.…”

ERK 1/2- and JNKs-dependent Synthesis of Interleukins 6 and 8 by Fibroblast-like Synoviocytes Stimulated with Protein I/II, a Modulin from Oral Streptococci, Requires Focal Adhesion Kinase

“…Purification of Protein I/II-Recombinant protein I/II of S. mutans OMZ 175 was purified from pHBsr-1-transformed Escherichia coli cell extract by gel filtration and immunoaffinity chromatography as previously described (31). The purity of the protein was checked by SDS-PAGE after staining with Coomassie Blue.…”

Involvement of alpha5beta1 integrins in interleukin 8 production induced by oral viridans streptococcal protein I/IIf in cultured endothelial cells

“…Recombinant protein I/IIf and protein PD 26 from Streptococcus mutans OMZ 175 were puri®ed from pHBsr-1or pSR D 26 transformed E. coli cell extract, respectively (Ogier et al, 1989), by ammonium sulphate precipitation, followed by fractionation on an anti-protein I/IIf IgG Sepharose column and further puri®cation by anion exchange chromatography (Chatenay-Rivauday et al, 1998). Protein I/IIf migrated as a single band with an apparent molecular mass of 195 kDa, as checked by SDS±PAGE after staining with Coomassie blue.…”

“…Moreover, we and others have demonstrated the capacity of protein I/IIf to bind to extracellular matrix proteins, such as ®bronectin, collagen I and laminin (Love et al, 1997;Sciotti et al, 1997), and to epithelial, endothelial and monocytic cells (Soell et al, 1994;Vernier et al, 1996). We demonstrated recently that the speci®c binding of protein I/IIf from Streptococcus mutans serotype f to monocytic THP-1 cells occurs via lectin interactions speci®c for N-acetyl neuraminic acid (NANA) and fucose and is controlled by the extended V-region (Chatenay-Rivauday et al, 1998). We also showed that (i) the binding of members of the oral viridans streptococci to cells induces the release of pro-in¯ammatory cytokines by epithelial and endothelial cells (Vernier et al, 1996); (ii) protein I/IIf belongs to the recently de®ned class of modulins and exerts immunomodulatory effects on human cells (Soell et al, 1994;Vernier et al, 1996).…”

“…After binding to its cognate receptor, protein I/IIf promotes the induction of IL-8 from epithelial cells, IL-8 and IL-6 from endothelial cells, IL-1b, tumour necrosis factor alpha (TNF-a) and IL-6 from monocytes and the upregulation of E-selectin, ICAM-1 and VCAM-1 expression on endothelial cells (Vernier-Georgenthum et al, 1998). The lectin-like mode of recognition of monocyte receptor(s) by the V-extended region is a determinant in the release of TNF-a by THP-1 cells (Chatenay-Rivauday et al, 1998).…”