The 5-HT4 receptor agonist prucalopride does not facilitate cholinergic neurotransmission in circular and longitudinal smooth muscle preparations of equine mid-jejunum

The influence of a simulated digest of an equine dietary feed additive G's formula on contractile activity of gastric smooth muscle in vitro

“…Various studies have used in vitro force transduction to investigate the impact of prokinetic pharmaceuticals on specific areas of interest within the equine GIT (Lefebvre, Callens, Colen, & Delesalle, 2017; Nieto, Maher, Maher, Stanley, Larson, & Snyder, 2013; Nieto, Morales, et al, 2013; Nieto, Rakestraw, Snyder, & Vatistas, 2000). In vitro techniques using smooth muscle from different regions of the GIT to examine the impact of different dietary components and bioactive compounds have been performed for application in species of commercial relevance (Jalilzadeh‐Amin, Maham, Dalir‐Naghadeh, & Kheiri, 2012, 2012b) or those more typically used as human models such as murines and Guinea pigs (Amira et al, 2008; Badary, Awad, Sherief, & Hamada, 2006; Budriesi et al, 2010; Gharzouli & Holzer, 2004).…”

“…At the highest concentration of Ach, FA increased the rate of force generation compared to both CO and BL, which also points towards a sensitizing effect of the digest on the muscle tissue. Integral under the force curve is a useful characteristic of muscle contraction to measure (Harlow & Weekley, 1986; Lefebvre et al, 2017; Lu et al, 2018) and accounts for both the frequency and amplitude of phasic type contractions. By using AUC to compare the reaction of the smooth muscle CO and BL treated tissue, particularly for the full 8 min following Ach stimulation, pre‐treatment with the simulated digest of FA appears to have increased the rate and magnitude of tissue contraction as well as reduced muscle relaxation following stimulation with Ach.…”

“…At the highest concentration of Ach, FA increased the rate of force generation compared to both CO and BL, which also points towards a sensitizing effect of the digest on the muscle tissue. Integral under the force curve is a useful characteristic of muscle contraction to measure (Harlow & Weekley, 1986;Lefebvre et al, 2017;Lu et al, 2018) and accounts for both the frequency and amplitude of phasic type contractions.…”

Suspected lidocaine toxicity and the use of metoclopramide and neostigmine to treat post‐operative small intestinal ileus in a donkey

“…Post‐operative reflux (POR) and post‐operative ileus (POI) after colic surgery in horses are important causes of mortality and morbidity despite advances in our understanding of intestinal injury 1–3 . While little data are available in donkeys, the reported incidence of POI after colic surgery in horses ranges from 14% to 47%, 4–7 with mortality as high as 83%–86% 5,7 .…”

Efeito da eletroacupuntura sobre a motilidade e trânsito intestinal de equinos

“…O tratamento desta enfermidade é motivo de inúmeras e constantes pesquisas científicas (LAUS et al, 2017;LEFEBVRE et al, 2017;NIETO et al, 2013aNIETO et al, , 2013bOKAMURA et al, 2009). Há décadas sabe-se que o trato gastrointestinal possui uma diversidade de receptores, os quais irão originar as mais diversas respostas, quando ligados aos seus agonistas ou antagonistas.…”