The 5′-flanking sequence of the loach oocyte 5S rRNA gene contains a signal for effective transcription

Felgenhauer, P.E.; Sedman, Juhan; Shostak, N. G.; Timofeeva, M. Ya.; Lind, Anna-Karin; Bayev, A.A.

doi:10.1016/0378-1119(90)90186-u

Cited by 11 publications

(7 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2), suggesting a regulatory role, such as the control of transcription by RNA polymerase III (Nederby-Nielsen et al 1993;Pendás et al 1994;Suzuki et al 1996). Moreover, as in the NTSs of other fish species (Felgenhauser et al 1990;Pendás et al 1994;Sajdak et al 1998;Martins et al , 2001, a TATA-like sequence was observed upstream of the 5S rRNA gene in Brycon, at the -19 to -27 position (Fig. 2).…”

Section: Resultsmentioning

confidence: 87%

Molecular organization of 5S rDNA in fishes of the genusBrycon

Wasko

Martins²,

Wright³

et al. 2001

Genome

View full text Add to dashboard Cite

There are few reports on the genomic organization of 5S rDNA in fish species. To characterize the 5S rDNA nucleotide sequence and chromosomal localization in the Neotropical fishes of the genus Brycon, 5S rDNA copies from seven species were generated by PCR. The nucleotide sequences of the coding region (5S rRNA gene) and the nontranscribed spacer (NTS) were determined, revealing that the 5S rRNA genes were highly conserved, while the NTSs were widely variable among the species analyzed. Moreover, two classes of NTS were detected in each species, characterized by base substitutions and insertions-deletions. Using fluorescence in situ hybridization (FISH), two 5S rDNA chromosome loci that could be related to the two 5S rDNA NTS classes were observed in at least one of the species studied. 5S rDNA sequencing and chromosomal localization permitted the characterization of Brycon spp. and suggest a higher similarity among some of them. The data obtained indicate that the 5S rDNA can be an useful genetic marker for species identification and evolutionary studies.

show abstract

Section: Resultsmentioning

confidence: 87%

Molecular organization of 5S rDNA in fishes of the genusBrycon

Wasko

Martins²,

Wright³

et al. 2001

Genome

View full text Add to dashboard Cite

show abstract

“…Upstream (5Ј) control regions have been defined for the somatic 5S of X. laevis (Oei and Pieler 1990), the loach [Misgurnus fossilis (Felgenhauer et al 1990)], and the silkworm [Bombyx mori (Morton and Sprague 1984)]. Study of deletion mutants has shown that upstream control elements are required for wild-type expression.…”

Section: Resultsmentioning

confidence: 99%

“…Note 1: Testing of deletion mutants in transcription assays showed reduced levels of transcription when 5Ј flanking regions were shorter than these (Oei and Pieler 1990;Felgenhauer et al 1990;Morton and Sprague 1984). (Pieler et al 1987).…”

Section: Resultsmentioning

confidence: 99%

Intraindividual and Interspecies Variation in the 5S rDNA of Coregonid Fish

1998

View full text Add to dashboard Cite

This study was designed to characterize further the nontranscribed intergenic spacers (NTSs) of the 5S rRNA genes of fish and evaluate this marker as a tool for comparative studies. Two members of the closely related North American Great Lakes cisco species complex (Coregonus artedi and C. zenithicus) were chosen for comparison. Fluorescence in situ hybridization found the ciscoes to have a single multicopy 5S locus located in a C band-positive region of the largest submetacentric chromosome. The entire NTS was amplified from the two species by polymerase chain reaction with oligonucleotide primers anchored in the conserved 5S coding region. Complete sequences were determined for 25 clones from four individuals representing two discrete NTS length variants. Sequence analysis found the length variants to result from presence of a 130-bp direct repeat. No two sequences from a single fish were identical. Examination of sequence from the coding region revealed two types of 5S genes in addition to pseudogenes. This suggests the presence of both somatic and germline (oocyte) forms of the 5S gene in the genome of Coregonus. The amount of variation present among NTS sequences indicates that accumulation of variation (mutation) is greater in this multicopy gene than is gene conversion (homogenization). The high level of sequence variation makes the 5S NTS an inappropriate DNA sequence for comparisons of closely related taxa.

show abstract

“…In various vertebrate species, a conserved external promoter sequence, a D-box in mammals, is known to be present in the NTS upstream of the 5S rRNA gene (Hart and Folk 1982;Nielsen et al 1993;Hallenberg et al 1994;Jensen and Frederiksen 2000;Frederiksen and Andersen 2003;Fujiwara et al 2006), besides an internal control region that functions as a promoter for transcription of the gene by RNA polymerase III (Hallenberg et al 1994). Also, a similar cis-acting element influencing the transcriptional activity is located in the region between position -18 and the transcription start point in the 5S rRNA gene of loach oocyte (Felgenhauer et al 1990). Taking altogether, the conserved NTS portion of the bitterling 5S rDNA may function as an external promoter such as a mammalian D-box.…”

Section: Discussionmentioning

confidence: 96%

Molecular organization of 5S rDNA in bitterlings (Cyprinidae)

et al. 2008

View full text Add to dashboard Cite

Molecular organization and nucleotide sequences of the 5S rRNA gene and NTS were investigated in freshwater fish, bitterlings (Acheilognathinae), including 10 species/subspecies of four genera, Acheilognathus, Pseudoperilampus, Rhodeus, and Tanakia, to understand the evolutionary trait of 5S rDNA arrays. Southern hybridization analysis revealed a general trend with tandem repeats of 5S rDNA in all the examined bitterlings. Sequence analysis demonstrated a conserved 120 bp sequence of the 5S rRNA gene and a short NTS of 56-67 bp with two distinct portions, a conserved (5'-flanking portion; at positions -1 to -38) and a variable part (3'-flanking portion), in 6 of 10 species/subspecies examined. The conserved NTS region was most likely an external promoter so far observed in various vertebrates, whereas the variable NTS region could be divided into two types due to its nucleotide polymorphisms. Molecular phylogeny using the 5S rRNA gene and NTS sequences suggested the occurrence of 5S rDNA duplication before speciation and a concerted evolution for the gene and conserved NTS regions, but a birth-and-death process to maintain the variable NTS region. Thus, the 5S rDNA in the examined bitterlings might have evolved under a mixed process of evolution.

show abstract

The 5′-flanking sequence of the loach oocyte 5S rRNA gene contains a signal for effective transcription

Cited by 11 publications

References 26 publications

Molecular organization of 5S rDNA in fishes of the genusBrycon

Molecular organization of 5S rDNA in fishes of the genusBrycon

Intraindividual and Interspecies Variation in the 5S rDNA of Coregonid Fish

Molecular organization of 5S rDNA in bitterlings (Cyprinidae)

Contact Info

Product

Resources

About