The 308-nm excimer laser stimulates melanogenesis via the wnt/β-Catenin signaling pathway in B16 cells

Li, Lili; Liang, Yen Nan; Zhang, Donghong; Wang, Chen; Pan, Nannan; Hong, Jiqiong; Xiao, Hewei; Xie, Zhi

doi:10.1080/09546634.2019.1572861

Cited by 17 publications

(16 citation statements)

References 25 publications

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“…Melanin metabolic abnormalities play a crucial role in the development of vitiligo. 2,3 Abnormal melanin loss leads to vitiligo associated pathological processes. 4 Therefore, therapies that promote melanogenesis and melanocyte proliferation have been considered as the most promising treatment methods for vitiligo.…”

Section: Introductionmentioning

confidence: 99%

<p>308-nm Excimer Laser Plus Platelet-Rich Plasma for Treatment of Stable Vitiligo: A Prospective, Randomized Case–Control Study</p>

Deng¹,

Li²,

Yang³

2020

CCID

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Purpose: 308-nm excimer laser has a confirmed treatment effect on vitiligo. Platelet-rich plasma (PRP) is an autologous preparation which contains a variety of growth factors. The effect of 308-nm excimer laser combined with PRP on vitiligo has been rarely reported. This study investigated the effect of PRP combined with 308-nm excimer laser on stable vitiligo. Patients and Methods: A total of 60 patients with localized stable vitiligo who received treatment at Beijing Friendship Hospital and Xi'an Vitiligo Specialist Hospital between May 2019 and January 2020 were consecutively enrolled. They were equally randomized into three groups according to different treatment methods: intradermal PRP injection (group I), 308-nm excimer laser alone (group II), and 308-nm excimer laser plus PRP injection (group III). All treatments lasted for 3 months. At 3 months after treatment, clinical assessments were performed in terms of the visual analogue scale (VAS) score, repigmentation response and side effects. Results: The VAS scores showed significant differences among the three groups (P<0.001), with the highest score in group III, followed by group II and then group I. Repigmentation responses also showed significant differences among the groups (P<0.001), and the best effect was observed in group III. No side effects were reported in any of the groups. Conclusion: The effect of PRP combined with 308-nm excimer laser on stable vitiligo is significantly better than that of PRP and 308-nm excimer laser alone. It is safe and satisfactorily tolerant.

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Section: Introductionmentioning

confidence: 99%

<p>308-nm Excimer Laser Plus Platelet-Rich Plasma for Treatment of Stable Vitiligo: A Prospective, Randomized Case–Control Study</p>

Deng¹,

Li²,

Yang³

2020

CCID

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“…Recent proof has shown that vitiligo is attributed to the disruption of melanogenesis and metabolic function . The abnormal loss of melanin outcomes in vitiligo‐related pathological procedures .…”

Section: Introductionmentioning

confidence: 99%

“…2 Recent proof has shown that vitiligo is attributed to the disruption of melanogenesis and metabolic function. 3 The abnormal loss of melanin outcomes in vitiligo-related pathological procedures. 4 The promotion of melanocyte proliferation and melanogenesis enhancement is, therefore, a prospective vitiligo therapeutic strategy.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of combined excimer laser and platelet‐rich plasma for the treatment of nonsegmental vitiligo: A prospective comparative study

Khattab

Abdelbary

Fawzi

2019

J of Cosmetic Dermatology

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Background Excimer laser therapy of vitiligo generally takes months to years to achieve excellent outcomes. Platelet‐rich plasma is an autologous preparation with a focus on various growth factors that can help with vitiligo repigmentation. Objectives To assess the additive effect of PRP in the therapy of vitiligo on the results of the excimer laser. Patients and Methods A comparative study included 52 patients (8 males and 44 females) in two groups with stable (no new lesion for 6 months), nonsegmental and symmetrical vitiligo. Group I (26 patients): The patient was treated by intradermal PRP injection and the excimer laser, while group II (26 patients) was treated with the excimer laser only. The PRP injection was repeated every 3 weeks for 4 months and excimer laser two times a week and for 16 weeks till complete response. VAS for patient's satisfaction assessment, safety assessment for complications, and follow‐up for 3 months was done. Clinical (repigmentation response) and histopathological assessment was done. Results There was a higher statistically significant treatment response in group I compared with group II. In addition, a statistically significant correlation between the treatment response and the lesion site in group I (P < .000). A significant difference in VAS between both groups (P < .000). Few the side effects were reported. Conclusion The combination of PRP and excimer laser phototherapy is an efficient vitiligo treatment as PRP increases the excimer laser impact and also improves the result.

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“…However, these therapies have severe side effects when used for a long time [6]. As research on vitiligo continues to unveil molecular factors involved in its progression, upregulation of melanogenesis and promotion of melanocyte proliferation are primary goals of therapeutic strategies for this condition [7,8].…”

Section: Introductionmentioning

confidence: 99%

Suppression of FADS1 induces ROS generation, cell cycle arrest, and apoptosis in melanocytes: implications for vitiligo

Tang

et al. 2019

Aging

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Vitiligo is a potentially serious condition characterized by loss of melanin and death of melanocytes. To identify potential therapeutic targets for vitiligo, we conducted a microarray analysis of three human vitiligo specimens and paired adjacent normal tissues. Because we found that the fatty acid desaturase 1 (FADS1) gene was downregulated in vitiligo specimens, we carried out experiments to assess its role in melanocyte replication and survival. RT-qPCR was used to verify that FADS1 expression was lower in vitiligo-affected tissues and vitiligo melanocyte PIG3V cells than in matched controls or normal human epidermal PIG1 melanocytes. In addition, CCK-8, immunofluorescence, western blot and flow cytometry assay were used to detect the proliferation and apoptosis in PIG1 cells respectively. Overexpression of FADS1 promoted proliferation of PIG3V melanocytes, while FADS1 silencing inhibited proliferation and induced cell death in PIG1 melanocytes. Increased ROS generation; induction of mitochondrial-mediated apoptosis via upregulation of Bax and active caspases 3 and 9 and downregulation of Bcl-2; and cell cycle arrest via downregulation of c-Myc and Cyclin D1 and upregulation of p21 were all enhanced after FADS1 silencing in PIG1 melanocytes. These findings implicate FADS1 downregulation in the pathogenesis of vitiligo and may open new avenues for its treatment.

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The 308-nm excimer laser stimulates melanogenesis via the wnt/β-Catenin signaling pathway in B16 cells

Cited by 17 publications

References 25 publications

<p>308-nm Excimer Laser Plus Platelet-Rich Plasma for Treatment of Stable Vitiligo: A Prospective, Randomized Case–Control Study</p>

<p>308-nm Excimer Laser Plus Platelet-Rich Plasma for Treatment of Stable Vitiligo: A Prospective, Randomized Case–Control Study</p>

Evaluation of combined excimer laser and platelet‐rich plasma for the treatment of nonsegmental vitiligo: A prospective comparative study

Suppression of FADS1 induces ROS generation, cell cycle arrest, and apoptosis in melanocytes: implications for vitiligo

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