The 3′UTR‐derived sRNA RsaG coordinates redox homeostasis and metabolism adaptation in response to glucose‐6‐phosphate uptake in Staphylococcus aureus

Abstract

Staphylococcus aureus is usually described as an extracellular opportunistic pathogen, infecting a wide range of organs and tissues.However, it also invades and replicates in various phagocytic or nonphagocytic host cells (Hamza & Li, 2014). To be successful as a pathogen, this bacterium needs to adapt to the hostile environment of the host and must acquire imposed nutriments for its survival.Consequently, the staphylococcal genome encodes several transporters for metabolites (sugars, metals, amino acids, etc.), which are

Thirty Years of sRNA-Mediated Regulation in Staphylococcus aureus: From Initial Discoveries to In Vivo Biological Implications

“…RsaC was found to repress the expression of the superoxide dismutase SodA, which is involved in the oxidative-stress response [40]. A recent study of the RsaG targetome revealed mRNAs of the transcription factors Rex, CcpA, and SarA as molecular targets [42]. Interestingly, studies conducted on both RsaI and RsaG showed that these two sRNAs interact together, which suggests complex sRNA regulatory networks (see Section 6 of this review).…”

“…The authors identified mRNAs encoding transcription factor MgrA, the SsaA protein involved in peptidoglycan synthesis, and the anti-inflammatory protein FLIPr, as the primary targets. A similar approach was used to identify the targetomes of RsaI, RsaC, SprY, and RsaG [39][40][41][42]. The use of RsaI as a bait allowed for the identification of mRNAs encoding a permease for glucose uptake (GlcU_2), along with Fn3K and IcaR, which are involved in protection against high concentrations of glucose and the repression of exopolysaccharide production, respectively [39].…”

“…The authors identified mRNAs encoding transcription factor MgrA, the SsaA protein involved in peptidoglycan synthesis, and the anti-inflammatory protein FLIPr, as the primary targets. A similar approach was used to identify the targetomes of RsaI, RsaC, SprY, and RsaG [39][40][41][42]. The Hybrid-trap-seq was developed a few years ago and has enabled the identification of RsaE targets [43].…”

“…To date, it is the sole sRNA group for which extensive data are available regarding the targetome through the implementation of MAPS [36,105]. Consequently, some direct sRNA targets were discovered in vitro for RsaA, RsaI, RsaC, and RsaG [38][39][40]42].…”

“…RsaG is the last Rsa sRNA characterized so far [42]. RsaG is highly expressed in response to extracellular glucose-6-phosphate (G6P), which is the activated form of glucose [39], but it is not essential for G6P uptake and catabolism [42].…”