The 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active copies of the widespread insertion sequence IS6100

Tauch, Andreas; Götker, Susanne; Pühler, Alfred; Kalinowski, Jörn; Thierbach, Georg

doi:10.1016/s0147-619x(02)00120-8

Cited by 112 publications

(94 citation statements)

References 29 publications

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“…IS6100 was originally discovered in Mycobacterium fortuitum (accession number X53635) (18) and was subsequently found in several gram-negative and -positive bacteria (9,26). It has been reported that transposition of IS6100 stimulates genetic rearrangement (12).…”

Section: Discussionmentioning

confidence: 99%

Genetic Environments of the rmtA Gene in Pseudomonas aeruginosa Clinical Isolates

Yamane

Doi

Yokoyama

et al. 2004

Antimicrob Agents Chemother

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Nine Pseudomonas aeruginosa strains showing very high levels of resistance to various aminoglycosides have been isolated from clinical specimens in seven separate Japanese hospitals in five prefectures since 1997. These strains harbor the newly identified 16S rRNA methylase gene (rmtA). When an rmtA gene probe was hybridized with genomic DNAs of the nine strains digested with EcoRI, two distinct patterns were observed. The 11.1-and 15.8-kb regions containing the rmtA genes of strains AR-2 and AR-11, respectively, were sequenced and compared. In strain AR-2, a transposase gene-like sequence (sequence 1) and a probable tRNA ribosyltransferase gene (orfA) were located upstream of rmtA, and a Na ؉ /H ؉ antiporter gene-like sequence (sequence 2) was identified downstream of rmtA. This 6.2-kbp insert (the rmtA locus) was flanked by 262-bp ␥ elements. Part of the orfQ gene adjacent to an inverted repeat was found outside of the rmtA locus. In strain AR-11, the rmtA gene and sequence 2 were found, but the 5 end of the orfA gene was truncated and replaced with IS6100. An orfQ-orfI region was present on each side of the rmtA gene in strain AR-11. The G؉C content of the rmtA gene was about 55%, and since the newly identified rmtA gene may well be mediated by some mobile genetic elements such as Tn5041, further dissemination of the rmtA gene could become an actual clinical problem in the near future.

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Section: Discussionmentioning

confidence: 99%

Genetic Environments of the rmtA Gene in Pseudomonas aeruginosa Clinical Isolates

Yamane

Doi

Yokoyama

et al. 2004

Antimicrob Agents Chemother

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“…Class 1 integron has been commonly reported in a large variety of clinical gram-negative organisms, including Acinetobacter, Aeromonas, Alcaligenes, Burkholderia, Campylobacter, Citrobacter, Enterobacter, Escherichia, Klebsiella, Mycobacterium, Pseudomonas, Salmonella, Serratia, Shigella and Vibrio, and occasionally observed Yu et al 1303 in Campylobacter jejuni, Providencia stuartii, Serratia marcescens, Stenotrophomonas maltophilia, with a broad distribution of 22-59% (Clark et al, 1999;Nandi et al, 2004;Nesvera J, 1998;Tauch et al, 2002;Arakawa et al, 1995;Lee et al, 2002;Chang et al, 2004;Li et al, 2002;Isabelle Plante, 2003;Ruiz et al, 2004). Regarded as a primary source of antimicrobial resistance genes and reservoirs and exchanging platforms of resistance genes within microbial populations, the role of class 1 integron in the dissemination of antibiotic resistance genes had been well investigated and documented in a large number of gram-negative bacteria (Labbate et al, 2009;Maguire et al, 2001;Martinez-Freijo et al, 1998;Sallen et al, 1995).…”

Section: Class 1 Integronmentioning

confidence: 99%

Role of integrons in antimicrobial resistance: A review

Gao¹,

Li²,

Liu³

et al. 2013

Afr. J. Microbiol. Res.

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Antibiotic resistance in bacteria still remains one of the leading concerns in global public health. Horizontal transfer of resistance genes is considered to be a major cause to facilitate the rapid spread of antibiotic resistance in microbes. In recent years, the role of integrons as mobile genetic elements playing a central role in horizontal transfer of antibiotic resistance have been well studied and documented. Nevertheless, up to date, most of the available investigations and studies on integron had focused on class 1 integrons of Gram-negative microorganisms. In the last decade, class 4 integrons (also known as super integrons) has been considered a major concern in antimicrobial resistance, as well as, a significant factor in bacterial genome evolution; however, most reports had been limited to Vibrio. Thus, this review is aimed at summarizing the occurrence and prevalence of integrons in bacterial isolates, with the focus on class 1 integron within Gram-positive bacteria, class 2 and 3 integrons.

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“…Tet(I) has not been sequenced, but phenotypic studies suggest it encodes an efflux pump (Jones et al, 1992a). The protein Tet(33) found in gram-positive Corynebacterium glutamicum revealed amino acid sequence homology to the group 1 tetracycline efflux systems, especially to Tet(Z) (Tauch et al, 2002).…”

Section: Active Effluxmentioning

confidence: 99%

“…The gene tet(33) was found on the 27.8-kb plasmid pTET3 from Corynebacterium glutamicum where it was flanked by identical copies of the widespread insertion sequence IS6100 (Tauch et al, 2002). The amino acid sequence of the encoded protein Tet(33) has shown a homology to the group 1 of tetracycline efflux systems, with the highest similarity to Tet(Z), also found in the same species.…”

Section: Active Efflux Determinants Of Grampositive Bacteriamentioning

confidence: 99%

Tetracyclines in veterinary medicine and bacterial resistance to them

Michalova¹,

PNovotna²,

Schlegelová³

2004

Vet. Med.

119

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Since their discovery in 1945, tetracyclines have been used extensively in the therapy and prophylaxis of infectious diseases and as growth promoters. These wide applications have led to the equally fast spread of tetracycline resistant strains of gram-positive and gram-negative bacterial genera, including strains belonging to pathogenic as well as nonpathogenic species. Nonpathogenic bacteria could act as a reservoir of resistance determinants, which can be disseminated by horizontal transfer into pathogens. More than thirty different tetracycline resistance genes have been characterized. They encode two major mechanisms of resistance: 1 -active efflux of the antibiotic, and 2 -protection of ribosomes. Further mechanisms of tetracycline resistance include enzymatic inactivation of antibiotic, permeability barriers, mutations or multidrug transporter systems. Effective horizontal spread is favoured by the location of tetracycline resistance genes on mobile genetic elements such as plasmids and transposons. Their exchange, enhanced by the use of tetracyclines, is observed between bacteria of the same or different species and genera as well. Thus, questions of reevaluating and global reducing of tetracyclines in human and animal healthcare and food production are extensively discussed.

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The 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active copies of the widespread insertion sequence IS6100

Cited by 112 publications

References 29 publications

Genetic Environments of the rmtA Gene in Pseudomonas aeruginosa Clinical Isolates

Genetic Environments of the rmtA Gene in Pseudomonas aeruginosa Clinical Isolates

Role of integrons in antimicrobial resistance: A review

Tetracyclines in veterinary medicine and bacterial resistance to them

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