The (18–48)-Fragment of human parathyroid hormone is a partial agonist for cAMP-Dependent actions with retained mitogenic function

Duvos, C.; Scutt, Andrew; Mayer, Hubert

doi:10.1016/s8756-3282(95)00266-9

Cited by 6 publications

(3 citation statements)

References 14 publications

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“…In isolation, neither hPTH-(53-84) nor hPTH-(28-48) had any affect calcium release however, both peptides significantly induced ALP activity [13,14]. Calcium levels in the culture system were optimised such that calcium release induced by PTH-(1-34) were maximised.…”

Section: Discussionmentioning

confidence: 99%

“…In this investigation we have demonstrated that the PTH fragments, hPTH-(53-84) and hPTH-(28-48), antagonised the effects of hPTH-(1-34) on calcium release and ALP activity, which are known to be cAMP mediated. In isolation, neither hPTH-(53-84) nor hPTH-(28-48) had any affect calcium release however, both peptides significantly induced ALP activity [13,14]. Calcium levels in the culture system were optimised such that calcium release induced by PTH-(1-34) were maximised.…”

Section: Discussionmentioning

confidence: 99%

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hPTH‐fragments (53–84) and (28–48) antagonize the stimulation of calcium release and repression of alkaline phosphatase activity by hPTH‐(1–34) in vitro

Duvos¹,

Scutt²,

Mayer³

2006

FEBS Letters

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Different C-terminal fragments of parathyroid hormone (PTH)-(1-84) in blood participate in the regulation of calcium homeostasis by PTH-(1-84), and an antagonizing effect for the large carboxyl-terminal parathyroid hormone (C-PTH)-fragment (7-84) on calcium release has been described in vivo and in vitro. In this study the smaller C-PTH-fragment (53-84) and mid-regional PTH fragment (28-48), which represent discrete areas of activity in the PTH-(7-84) molecule, were assayed for their effects on calcium release and alkaline phosphatase (ALP) activity in a chick bone organ culture system. Neither PTH-(28-48) nor PTH-(53-84) had any effect on calcium release into the medium and both fragments stimulated ALP activity in the bone tissue, suggesting that the cAMP/ PKA signalling pathway was not affected by these fragments. However they suppressed the calcium release induced by PTH-(1-34) and attenuated the down regulation of ALP activity caused by PTH-(1-34), suggesting that the effect on the cAMP/PKA signalling pathway may be indirectly. In conclusion, the study shows that the PTH-fragments (53-84) and (28-48) antagonize the PTH-(1-34) induced effects on calcium release and inhibition of ALP activity in a chick bone organ culture system.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

hPTH‐fragments (53–84) and (28–48) antagonize the stimulation of calcium release and repression of alkaline phosphatase activity by hPTH‐(1–34) in vitro

Duvos¹,

Scutt²,

Mayer³

2006

FEBS Letters

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“…More recent in vivo evidence of anabolic effect of PTH was obtained by Kalu and Liu [24] who showed large increases in the radiological density of metaphyseal bone in rats. Studies in animals have indicated that the anabolic effects of PTH may be related to its mitogenic actions [25]. …”

Section: Introductionmentioning

confidence: 99%

Synergistic Effect of Parathyroid Hormone and Growth Hormone on Trabecular and Cortical Bone Formation in Hypophysectomized Rats

Guevarra¹,

Yeh²,

Magana³

et al. 2010

Horm Res Paediatr

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Background/Aims: Growth hormone (GH) deficiency in pediatric patients results in short stature and osteopenia. We postulated that the GH and parathyroid hormone (PTH) combination would result in improvement in bone growth and bone formation. Methods: Forty hypophysectomized female rats at age 8 weeks were divided into hypophysectomy (HX), HX + PTH (62.5 µg/kg, s.c. daily), HX + GH (3.33 mg/kg, s.c. daily), and HX + PTH + GH for a 4-week study. Results: GH increased body weight, bone growth, bone mineral content (BMC) and bone mineral density (BMD), whereas PTH increased BMC and BMD without a significant effect on bone size. GH increased both periosteal and endocortical bone formation and cortical size, while PTH increased only endocortical bone formation. GH mitigated the trabecular bone loss by increasing bone formation, while PTH increased bone mass by increasing bone formation and suppressing osteoclast number per bone area. The result of combined intervention shows an increase in trabecular, periosteal and endocortical bone formation and suppression of bone resorption resulting in a synergistic effect on increasing trabecular and cortical bone volume and BMD. Conclusion: The combination treatment of PTH and GH increases bone growth, bone formation, decreases bone resorption and has a synergistic effect on increasing bone density and bone mass.

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Bone mineral density, osteopenia, and osteoporosis in the rhesus macaques of Cayo Santiago

Cerroni

Tomlinson

Turnquist

et al. 2000

Am. J. Phys. Anthropol.

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This cross-sectional study investigates metabolic bone disease and the relationship between age and bone mineral density (BMD) in males and females of a large, well-documented skeletal population of free-ranging rhesus monkeys (Macaca mulatta), from the Caribbean Primate Research Center Museum collection from Cayo Santiago, Puerto Rico. The sample consists of 254 individuals aged 1.0-20+ years. The data consist of measurements of bone mineral content and bone mineral density, obtained from dual-energy X-ray absorptiometry (DEXA), of the last lumbar vertebra from each monkey. The pattern of BMD differs between male and female rhesus macaques. Females exhibit an initial increase in BMD with age, with peak bone density occurring around age 9.5 years, and remaining constant until 17.2 years, after which there is a steady decline in BMD. Males acquire bone mass at a faster rate, and attain a higher peak BMD at an earlier age than do females, at around 7 years of age, and BMD remains relatively constant between ages 7-18.5 years. After age 7 there is no apparent effect of age on BMD in the males of this sample; males older than 18.5 years were excluded due to the presence of vertebral osteophytosis, which interferes with DEXA. The combined frequency of osteopenia and osteoporosis in this population is 12.4%. BMD values of monkeys with vertebral wedge fractures are generally higher than those of virtually all of the nonfractured osteopenic/osteoporotic individuals, thus supporting the view that BMD as measured by DEXA is a useful but imperfect predictor of fracture risk, and that low BMD may not always precede fractures in vertebral bones. Other factors such as bone quality (i.e., trabecular connectivity) should also be considered. The skeletal integrity of a vertebra may be compromised by the loss of key trabeculae, resulting in structural failure, but the spine may still show a BMD value within normal limits, or within the range of osteopenia.

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The (18–48)-Fragment of human parathyroid hormone is a partial agonist for cAMP-Dependent actions with retained mitogenic function

Cited by 6 publications

References 14 publications

hPTH‐fragments (53–84) and (28–48) antagonize the stimulation of calcium release and repression of alkaline phosphatase activity by hPTH‐(1–34) in vitro

hPTH‐fragments (53–84) and (28–48) antagonize the stimulation of calcium release and repression of alkaline phosphatase activity by hPTH‐(1–34) in vitro

Synergistic Effect of Parathyroid Hormone and Growth Hormone on Trabecular and Cortical Bone Formation in Hypophysectomized Rats

Bone mineral density, osteopenia, and osteoporosis in the rhesus macaques of Cayo Santiago

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