The 14-3-3 Proteins Encoded by the BMH1 and BMH2 Genes are Essential in the Yeast Saccharomyces cerevisiae and Can be Replaced by a Plant Homologue

Heusden, G. Paul H. van; Griffiths, Dominic J. F.; Ford, Jon C.; Chin-A-Woeng, Thomas F. C.; Schrader, Peter A. T.; Carr, Anthony; Steensma, H. Yde

doi:10.1111/j.1432-1033.1995.tb20435.x

Cited by 8 publications

(7 citation statements)

References 45 publications

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“…In order to study the role of 14-3-3 proteins in transcription regulation, we investigated the effect of mutation of the BMH genes on the genome-wide transcription profile. Such studies are complicated by the fact that deletion of both BMH genes is lethal in most laboratory strains [12,13]. In our previous study, we constructed a temperature-sensitive bmh2 mutant by deleting both BMH genes and introduction of a plasmid containing a temperature-sensitive bmh2 allele [24].…”

Section: Construction Of a Bmh2 Mutantmentioning

confidence: 99%

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Regulation of transcription bySaccharomyces cerevisiae14-3-3 proteins

Bruckmann

Steensma

Mattos

et al. 2004

Biochemical Journal

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14-3-3 proteins form a family of highly conserved eukaryotic proteins involved in a wide variety of cellular processes, including signalling, apoptosis, cell-cycle control and transcriptional regulation. More than 150 binding partners have been found for these proteins. The yeast Saccharomyces cerevisiae has two genes encoding 14-3-3 proteins, BMH1 and BMH2. A bmh1 bmh2 double mutant is unviable in most laboratory strains. Previously, we constructed a temperature-sensitive bmh2 mutant and showed that mutations in RTG3 and SIN4, both encoding transcriptional regulators, can suppress the temperature-sensitive phenotype of this mutant, suggesting an inhibitory role of the 14-3-3 proteins in Rtg3-dependent transcription [van Heusden and Steensma (2001) Yeast 18, 1479-1491]. In the present paper, we report a genome-wide transcription analysis of a temperature-sensitive bmh2 mutant. Steady-state mRNA levels of 60 open reading frames were increased more than 2.0-fold in the bmh2 mutant, whereas those of 78 open reading frames were decreased more than 2.0-fold. In agreement with our genetic experiments, six genes known to be regulated by Rtg3 showed elevated mRNA levels in the mutant. In addition, several genes with other cellular functions, including those involved in gluconeogenesis, ergosterol biosynthesis and stress response, had altered mRNA levels in the mutant. Our data show that the yeast 14-3-3 proteins negatively regulate Rtg3-dependent transcription, stimulate the transcription of genes involved in ergosterol metabolism and in stress response and are involved in transcription regulation of multiple other genes.

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Section: Construction Of a Bmh2 Mutantmentioning

confidence: 99%

“…The yeast Saccharomyces cerevisiae has two genes, BMH1 and BMH2, encoding 14-3-3 proteins [11][12][13][14]. A bmh1 bmh2 disruption is lethal in most, but not all, laboratory strains, and the lethal bmh1 bmh2 disruption can be complemented by at least four of the Arabidopsis isoforms and by a human and a Dictyostelium isoform [15,16].…”

Section: Introductionmentioning

confidence: 99%

Regulation of transcription bySaccharomyces cerevisiae14-3-3 proteins

Bruckmann

Steensma

Mattos

et al. 2004

Biochemical Journal

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“…In Saccharomyces cerevisiae and Scizosaccharomyces pombe there are two 14-3-3 genes designated BMH1 and BMH2 (van Heusden et al, 1992(van Heusden et al, , 1995 and rad24 and rad25 (Ford et al, 1992), respectively. In these fungi, deletion of one 14-3-3 gene has little effect but disruption of both renders the cell inviable in most strains.…”

Section: Introductionmentioning

confidence: 99%

14-3-3 regulates the G2/M transition in the basidiomycete Ustilago maydis

Mielnichuk

Pérez-Martı́n

2008

Fungal Genetics and Biology

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“…In our previous study we showed that in the presence of phosphate GFP-tagged SPL2 is expressed in more than 90% of the cells, and in a small number of cells no expression is seen [22]. On the other hand, upon deletion of BMH1, encoding the major 14-3-3 protein isoform [23][24][25], SPL2-GFP is expressed in only 20 to 30% of the cells and the majority of the cells are not expressing SPL2-GFP. After 60 min of phosphate or potassium starvation expression of SPL2-GFP is strongly induced in all cells, both in the wild type strain and in the bmh1Δ deletion mutant.…”

Section: Introductionmentioning

confidence: 95%

Cell-to-cell heterogeneity of phosphate gene expression in yeast is controlled by alternative transcription, 14-3-3 and Spl2

Crooijmans

Hensen

et al. 2021

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanism

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Dependent on phosphate availability the yeast Saccharomyces cerevisiae expresses either low or high affinity phosphate transporters. In the presence of phosphate yeast cells still express low levels of the high affinity phosphate transporter Pho84. The regulator Spl2 is expressed in approximately 90% of the cells, and is not expressed in the remaining cells. Here we report that deletion of RRP6, encoding an exonuclease degrading noncoding RNA, or BMH1, encoding the major 14-3-3 isoform, resulted in less cells expressing SPL2 and in increased levels of RNA transcribed from sequences upstream of the SPL2 coding region. SPL2 stimulates its own expression and that of PHO84 ensuing a positive feedback. Upon deletion of the region responsible for upstream SPL2 transcription almost all cells express SPL2. These results indicate that the cell-to-cell variation in PHO84 and SPL2 expression is dependent on a specific part of the SPL2 promoter and is controlled by Bmh1 and Spl2.

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The 14-3-3 Proteins Encoded by the BMH1 and BMH2 Genes are Essential in the Yeast Saccharomyces cerevisiae and Can be Replaced by a Plant Homologue

Cited by 8 publications

References 45 publications

Regulation of transcription bySaccharomyces cerevisiae14-3-3 proteins

Regulation of transcription bySaccharomyces cerevisiae14-3-3 proteins

14-3-3 regulates the G2/M transition in the basidiomycete Ustilago maydis

Cell-to-cell heterogeneity of phosphate gene expression in yeast is controlled by alternative transcription, 14-3-3 and Spl2

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