The 13C-Butyrate Breath Test: a New Non-Invasive Method for Assessing Colitis in a Murine Model

13C‐butyrate breath test is a novel non‐invasive screening tool for anti‐inflammatory activity of kampo medicines

“…We have also confirmed that 13 C-butyrate breath test can assess the amelioration of inflammation after salicylazosulfapyridine treatment in DSS-induced murine colitis [13]. Therefore, 13 C-butyrate breath test could be used to easily screen anti-inflammatory kampo medicines with various combinations of crude herbs. We verified the applicability of this test by using it to study the effects of two kampo medicines (often prescribed for inflammatory bowel disorders) on the excretion of 13 CO 2 in DSS murine colitis.…”

“…In conclusion, 13 C-butyrate breath test is a useful new non-invasive method for evaluating anti-inflammatory kampo medicines in murine colitis. This method is a novel screening tool for the anti-inflammatory activity of uninvestigated kampo medicines.…”

“…This method is a novel screening tool for the anti-inflammatory activity of uninvestigated kampo medicines. 13 C-butyrate breath test (data given as mean AE SEM of 4-7 mice). ## P < 0.01, significantly different from the normal group; *P < 0.05 and **P < 0.01, significantly different from the control group.…”

“…The real‐time evaluation of the inflammatory phase and effectiveness of a therapeutic agent using an animal colitis model requires many experimental animals. We have previously established a new non‐invasive method, the 13 C‐butyrate breath test, which reduces the number of experimental animals needed to assess dextran sulfate sodium salt (DSS)‐induced murine colitis . We have also confirmed that 13 C‐butyrate breath test can assess the amelioration of inflammation after salicylazosulfapyridine treatment in DSS‐induced murine colitis .…”

“…We have previously established a new non-invasive method, the 13 C-butyrate breath test, which reduces the number of experimental animals needed to assess dextran sulfate sodium salt (DSS)-induced murine colitis [13]. We have also confirmed that 13 C-butyrate breath test can assess the amelioration of inflammation after salicylazosulfapyridine treatment in DSS-induced murine colitis [13]. Therefore, 13 C-butyrate breath test could be used to easily screen anti-inflammatory kampo medicines with various combinations of crude herbs.…”