The 1.9 Å crystal structure of the extracellular matrix protein Bap1 from Vibrio cholerae provides insights into bacterial biofilm adhesion

Kaus, Katherine; Biester, Alison; Chupp, Ethan; Lu, Jiongjiong; Visudharomn, Charlie; Olson, Rich

doi:10.1074/jbc.ra119.008335

Cited by 12 publications

(15 citation statements)

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“…Thus, a certain, as-yet-unidentified protein must be closely related to the maintenance of Staphylococcal biofilms. A recent report revealed that both matrix Bap 1 and Rbm C proteins produced by Vibrio cholerae play distinct adhesive roles in its biofilm formation ( Kaus et al, 2019 ). Wrobel et al (2020) have recently reported that two autotransporters of Yersinia ruckeri , which localize to the outer membrane, contribute to biofilm formation.…”

Section: Discussionmentioning

confidence: 99%

“…In Vibrio cholerae , the matrix proteins RbmA, RbmC, and Bap1, in addition to Vibrio polysaccharide and nucleic acids, are known as the major components of V. cholerae biofilm ( Fong et al, 2006 ; Fong and Yildiz, 2007 ; Berk et al, 2012 ). Recently, the crystal structure of Bap1 has also been elucidated ( Kaus et al, 2019 ). Thus, some bacterial biofilms are being analyzed at the molecular level to identify their components.…”

Section: Introductionmentioning

confidence: 99%

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Outer Membrane Vesicles Released From Aeromonas Strains Are Involved in the Biofilm Formation

et al. 2021

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Aeromonas spp. are Gram-negative rod-shaped bacteria ubiquitously distributed in diverse water sources. Several Aeromonas spp. are known as human and fish pathogens. Recently, attention has been focused on the relationship between bacterial biofilm formation and pathogenicity or drug resistance. However, there have been few reports on biofilm formation by Aeromonas. This study is the first to examine the in vitro formation and components of the biofilm of several Aeromonas clinical and environmental strains. A biofilm formation assay using 1% crystal violet on a polystyrene plate revealed that most Aeromonas strains used in this study formed biofilms but one strain did not. Analysis of the basic components contained in the biofilms formed by Aeromonas strains confirmed that they contained polysaccharides containing GlcNAc, extracellular nucleic acids, and proteins, as previously reported for the biofilms of other bacterial species. Among these components, we focused on several proteins fractionated by SDS-PAGE and determined their amino acid sequences. The results showed that some proteins existing in the Aeromonas biofilms have amino acid sequences homologous to functional proteins present in the outer membrane of Gram-negative bacteria. This result suggests that outer membrane components may affect the biofilm formation of Aeromonas strains. It is known that Gram-negative bacteria often release extracellular membrane vesicles from the outer membrane, so we think that the outer membrane-derived proteins found in the Aeromonas biofilms may be derived from such membrane vesicles. To examine this idea, we next investigated the ability of Aeromonas strains to form outer membrane vesicles (OMVs). Electron microscopic analysis revealed that most Aeromonas strains released OMVs outside the cells. Finally, we purified OMVs from several Aeromonas strains and examined their effect on the biofilm formation. We found that the addition of OMVs dose-dependently promoted biofilm formation, except for one strain that did not form biofilms. These results suggest that the OMVs released from the bacterial cells are closely related to the biofilm formation of Aeromonas strains.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Outer Membrane Vesicles Released From Aeromonas Strains Are Involved in the Biofilm Formation

et al. 2021

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“…More specifically, RmbC has a role in maintaining and stabilizing the biofilm [177]. A study using mutants lacking RbmC and its homolog Bap1 showed a change of colonial morphology and the loss of biofilm formation capacity [177,178]. On the other hand, RbmA controls the structure of the biofilm [9,179].…”

Section: Biofilm Maturationmentioning

confidence: 99%

The Secretome of Vibrio cholerae

Mathieu-Denoncourt¹,

Giacomucci²,

Duperthuy³

2021

Infections and Sepsis Development

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Vibrio cholerae is a facultative human pathogen responsible for the cholera disease which infects millions of people worldwide each year. V. cholerae is a natural inhabitant of aquatic environments and the infection usually occurs after ingestion of contaminated water or food. The virulence factors of V. cholerae have been extensively studied in the last decades and include the cholera toxin and the coregulated pilus. Most of the virulence factors of V. cholerae belong to the secretome, which corresponds to all the molecules secreted in the extracellular environment such as proteins, exopolysaccharides, extracellular DNA or membrane vesicles. In this chapter, we review the current knowledge of the secretome of V. cholerae and its role in virulence, colonization and resistance. In the first section, we focus on the proteins secreted through conventional secretion systems. The second and third sections emphasize on the membrane vesicles and on the secretome associated with biofilms.

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“…For V. cholerae, the biofilm matrix primarily consists of the long-chain polysaccharide VPS, which is bound by the major matrix protein RbmA (20)(21)(22). Further matrix components are extracellular DNA (23,24), the RbmC protein and several additional matrix proteins whose functions are mostly uncharacterized (25,26), as well as factors used for surface attachment of the cells, including Bap1, FraH, CraA, and MSHA pili (20,(27)(28)(29)(30)(31)(32). During V. cholerae biofilm growth, RbmA is known to be processed by the proteases HapA, PrtV, and IvaP (33,34), yet neither of these proteases is known to play a role in dispersal from abiotic surfaces (35).…”

Section: Introductionmentioning

confidence: 99%

Vibrio choleraebiofilm dispersal regulator causes cell release from matrix through type IV pilus retraction

Singh

Rode

Buffard

et al. 2021

Preprint

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The extracellular matrix is a defining feature of bacterial biofilms and provides structural stability to the community by binding cells to the surface and to each other. Transitions between bacterial biofilm initiation, growth, and dispersion require different regulatory programs, all of which result in modifications to the extracellular matrix composition, abundance, or functionality. However, the mechanisms by which individual cells in biofilms disengage from the matrix to enable their departure during biofilm dispersal are unclear. Here, we investigated active biofilm dispersal of Vibrio cholerae during nutrient starvation, resulting in the discovery of the conserved Vibrio biofilm dispersal regulator VbdR. We show that VbdR triggers biofilm dispersal by controlling cellular release from the biofilm matrix, which is achieved by inducing the retraction of the mannose-sensitive hemagglutinin (MSHA) type IV pili and the expression of a matrix protease IvaP. We further show that MSHA pili have numerous binding partners in the matrix and that the joint effect of MSHA pilus retraction and IvaP activity is necessary and sufficient for causing biofilm dispersal. These results highlight the crucial role of type IV pilus dynamics during biofilm dispersal and provide a new target for controlling V. cholerae biofilm abundance through the induction and manipulation of biofilm dispersal.

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The 1.9 Å crystal structure of the extracellular matrix protein Bap1 from Vibrio cholerae provides insights into bacterial biofilm adhesion

Cited by 12 publications

References 61 publications

Outer Membrane Vesicles Released From Aeromonas Strains Are Involved in the Biofilm Formation

Outer Membrane Vesicles Released From Aeromonas Strains Are Involved in the Biofilm Formation

The Secretome of Vibrio cholerae

Vibrio choleraebiofilm dispersal regulator causes cell release from matrix through type IV pilus retraction

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