The 1.4‐β‐glucan cellobiohydrolases of Trichoderma reesei QM 9414

The primary structure of a 1,4‐β‐glucan cellobiohydrolase from the fungus Trichoderma reesei QM 9414

“…Structural studies on other cellulolytic enzymes from T. reesei have revealed the same type of blockage in a second cellobiohydrolase (CBH II) [2] and in the main 1,4-fi-glucan glucanohydrolase [23].…”

“…The reaction was terminated at times 0, 1, 2,3,4,5,10,15,30 and 60 min with glacial acetic acid. Released amino acids were determined by amino acid analysis.…”

“…The cellobiohydrolase activity has been shown to be present as at least two structurally unrelated enzymes [ 1,2]. Under optimal cultur-+ To whom correspondence should be addressed Abbreviatto~; CBH I, 1,4-@-glucan cellobiohydrol~e; RCM, reduced and carboxymethylated ing conditions the most abundant cellobiohydrolase constitutes about 50% of the protein released into the culture medium [3].…”

Regulation of transcription of cellulases- and hemicellulases-encoding genes in Aspergillus niger and Hypocrea jecorina (Trichoderma reesei)

“…Filamentous fungi like Aspergillus niger and Hypocrea jecorina (Trichoderma reesei) have been shown to produce a wide spectrum of polysaccharide-hydrolytic enzymes. Of these fungi, A. niger is industrially used as a producer of many pectinases (Bussink et al 1992;Delgado et al 1992;Parenicová et al 2000) and hemicellulosedegrading enzymes, like xylanases and arabinases (Gielkens et al 1997;van Peij et al 1997), while H. jecorina is the most important organism used in cellulase production (Fägerstam and Pettersson 1980;Irwin et al 1993;Nidetzky et al 1994;Wood and McCrae 1979).…”

Separation of endo‐ and exo‐type cellulases using a new affinity chromatography method

“…Ion-exchange chromatographic methods have been used most often [ 1,2]. Affinity chromatography on mi'crocrystalline cellulose (Avicel) or on amorphous cellulose has been attempted by several authors .…”