Thalassobacillus, a genus of extreme to moderate environmental halophiles with biotechnological potential

Tuesta-Popolizio, Diego A.; Velázquez-Fernández, Jesús Bernardino; Rodríguez-Campos, Jacobo; Contreras-Ramos, Silvia Maribel

doi:10.1007/s11274-021-03116-0

Cited by 9 publications

(3 citation statements)

References 75 publications

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“…In relation to this group of microorganisms, it should be mentioned that the bacterium Thalassobacillus devorans was isolated for the first time from saline soils in southern Spain [ 70 ]. Many other species belonging to the genus Thalassobacillus have been isolated from various saline environments, such as a hypersaline lake, marine coastal tidal flat sediments, and salty animal skins [ 71 , 72 , 73 , 74 ]. Thalassobacillus bacteria are moderately halophilic.…”

Section: Discussionmentioning

confidence: 99%

New Insights into the Effect of Fipronil on the Soil Bacterial Community

et al. 2022

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Fipronil is a broad-spectrum insecticide with remarkable efficacy that is widely used to control insect pests around the world. However, its extensive use has led to increasing soil and water contamination. This fact is of concern and makes it necessary to evaluate the risk of undesirable effects on non-target microorganisms, such as the microbial community in water and/or soil. Studies using the metagenomic approach to assess the effects of fipronil on soil microbial communities are scarce. In this context, the present study was conducted to identify microorganisms that can biodegrade fipronil and that could be of great environmental interest. For this purpose, the targeted metabarcoding approach was performed in soil microcosms under two environmental conditions: fipronil exposure and control (without fipronil). After a 35-day soil microcosm period, the 16S ribosomal RNA (rRNA) gene of all samples was sequenced using the ion torrent personal genome machine (PGM) platform. Our study showed the presence of Proteobacteria, Actinobacteria, and Firmicutes in all of the samples; however, the presence of fipronil in the soil samples resulted in a significant increase in the concentration of bacteria from these phyla. The statistical results indicate that some bacterial genera benefited from soil exposure to fipronil, as in the case of bacteria from the genus Thalassobacillus, while others were affected, as in the case of bacteria from the genus Streptomyces. Overall, the results of this study provide a potential contribution of fipronil-degrading bacteria.

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Section: Discussionmentioning

confidence: 99%

New Insights into the Effect of Fipronil on the Soil Bacterial Community

et al. 2022

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“…The possibilities are endless given the great variability of conditions in which microorganisms can be found naturally. In the literature, we can find many examples of natural sources of isolation from hypersaline lakes, ponds contaminated with hydrocarbons, desert lands in Mexico, arctic seas, and forest floors [17,[20][21][22].…”

Section: Natural Sources Of Isolation Of Fermentative Biotechnologica...mentioning

confidence: 99%

Fundaments and Concepts on Screening of Microorganisms for Biotechnological Applications. Mini Review

et al. 2022

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Microbial biotechnology uses microorganisms and their derivatives to generate industrial and/or environmental products that impact daily life. Modern biotechnology uses proteomics, metabolomics, quantum processors, and massive sequencing methods to yield promising results with microorganisms. However, the fundamental concepts of microbial biotechnology focus on the specific search for microorganisms from natural sources and their correct analysis to implement large-scale processes. This mini-review focuses on the methods used for the isolation and selection of microorganisms with biotechnological potential to empathize the importance of these concepts in microbial biotechnology. In this work, a review of the state of the art in recent years on the selection and characterization of microorganisms with a basic approach to understanding the importance of fundamental concepts in the field of biotechnology was carried out. The proper selection of isolation sources and the design of suitable selection criteria according to the desired activity have generated substantial changes in the development of biotechnology for more than three decades. Some examples include Taq polymerase in the PCR method and CRISPR technology. The objective of this mini review is to establish general ideas for the screening of microorganisms based on basic concepts of biotechnology that are left aside in several articles and maintain the importance of the basic concepts that this implies in the development of modern biotechnology.

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“…Halophilic microorganisms are capable of surviving and proliferating in high-salt environments [1,2]. They possess unique physiological characteristics and adaptive mechanisms to withstand high salt conditions, including "salt-in" and "compatible-solute" [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Temporal dynamics of stress response in Halomonas elongata to NaCl shock: physiological, metabolomic, and transcriptomic insights

Yu,

Zhang,

Liu

et al. 2024

Microb Cell Fact

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Background The halophilic bacterium Halomonas elongata is an industrially important strain for ectoine production, with high value and intense research focus. While existing studies primarily delve into the adaptive mechanisms of this bacterium under fixed salt concentrations, there is a notable dearth of attention regarding its response to fluctuating saline environments. Consequently, the stress response of H. elongata to salt shock remains inadequately understood. Results This study investigated the stress response mechanism of H. elongata when exposed to NaCl shock at short- and long-time scales. Results showed that NaCl shock induced two major stresses, namely osmotic stress and oxidative stress. In response to the former, within the cell’s tolerable range (1–8% NaCl shock), H. elongata urgently balanced the surging osmotic pressure by uptaking sodium and potassium ions and augmenting intracellular amino acid pools, particularly glutamate and glutamine. However, ectoine content started to increase until 20 min post-shock, rapidly becoming the dominant osmoprotectant, and reaching the maximum productivity (1450 ± 99 mg/L/h). Transcriptomic data also confirmed the delayed response in ectoine biosynthesis, and we speculate that this might be attributed to an intracellular energy crisis caused by NaCl shock. In response to oxidative stress, transcription factor cysB was significantly upregulated, positively regulating the sulfur metabolism and cysteine biosynthesis. Furthermore, the upregulation of the crucial peroxidase gene (HELO_RS18165) and the simultaneous enhancement of peroxidase (POD) and catalase (CAT) activities collectively constitute the antioxidant defense in H. elongata following shock. When exceeding the tolerance threshold of H. elongata (1–13% NaCl shock), the sustained compromised energy status, resulting from the pronounced inhibition of the respiratory chain and ATP synthase, may be a crucial factor leading to the stagnation of both cell growth and ectoine biosynthesis. Conclusions This study conducted a comprehensive analysis of H. elongata’s stress response to NaCl shock at multiple scales. It extends the understanding of stress response of halophilic bacteria to NaCl shock and provides promising theoretical insights to guide future improvements in optimizing industrial ectoine production.

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Thalassobacillus, a genus of extreme to moderate environmental halophiles with biotechnological potential

Cited by 9 publications

References 75 publications

New Insights into the Effect of Fipronil on the Soil Bacterial Community

New Insights into the Effect of Fipronil on the Soil Bacterial Community

Fundaments and Concepts on Screening of Microorganisms for Biotechnological Applications. Mini Review

Temporal dynamics of stress response in Halomonas elongata to NaCl shock: physiological, metabolomic, and transcriptomic insights

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