Th1/Th2 PB balance and CD200 expression of patients with active severe alopecia areata

Abstract

Th1/Th2 peripheral blood balance and CD200 expression in patients with severe alopecia areata (SAA) in the active stage were investigated. Fifty patients with active SAA, 50 patients with stable SAA and 50 healthy controls were continuously selected and expression of Th1/Th2 of peripheral T lymphocytes, and peripheral B lymphocytes was detected by flow cytometry; RT-PCR was used to detect the expression of the PBMC CD200 mRNA and the expression of CD200 in hair follicles of alopecia area was detected by immunohistochemically staining; ELISA was used to detect expression levels of serum LFN-γ and serum interleukin (IL)-10. The expression of CD200 in patients with alopecia areata in active phase on CD3+ T lymphocytes and CD19+ B lymphocytes was significantly lower (P<0.05) than those in stable phase and of the control group. CD200 expression in patients with alopecia areata in stable phase on T lymphocytes was greatly lower than that of the control group (P<0.05). However, the comparison of expression of CD200 in patients with alopecia areata in stable phase on B lymphocytes with the control group were statistically non-significant. The level of the expression of CD200 mRNA in active phase was obviously lower than those of the other two groups and the difference was statistically significant (P<0.05); the moderate positive and strong positive percentage of CD200 in the active phase was significantly lower than those of the other two groups. Positive expression rate of CK15 among the three groups were compared with each other; the differences had no statistical significance. The level of LFN-γ in the active phase had obviously increased while the IL-10 level decreased significantly (P<0.05). In conclusion, the level of expression of CD200 on peripheral blood and hair follicle outer root sheath of patients with SAA was decreased. This may be associated with the imbalance of the Th1/Th2 equilibrium.

Differences in Intercellular Communication During Clinical Relapse and Gadolinium-Enhanced MRI in Patients With Relapsing Remitting Multiple Sclerosis: A Study of the Composition of Extracellular Vesicles in Cerebrospinal Fluid

“…CD19 and CD200 are both expressed on immature and naïve B lymphocytes ( Rasmussen et al, 2015 ). This double staining has never been associated with MS, but a reduction in the number of CD19/CD200 B lymphocytes has recently been described in the acute phase of severe alopecia areata, a hair follicle-specific Th1 cell-mediated autoimmune disease ( Gilhar and Kalish, 2006 ; Ma et al, 2017 ).…”

Evaluation of serum levels of IL-6, IL-10, and TNF-a in alopecia areata patients: A systematic review and meta-analysis

“…The levels of IL-10 in the serum of healthy subjects were higher than AA patients, but the difference was not significant (SMD = -0.22, 95% CI -0.95 to 0.50). Among six studies that report IL-10 levels in the sera of AA patients, one study shows reduced levels of IL-10 in AA patients compared to healthy controls 17 , one study shows a raised level 16 , and four studies show no significant difference 14,18,25, 26 . There was a significant elevation of IL-6 in the serum of AA patients compared to healthy controls (SMD = 1.57, 95% CI 0.17 to 2.97).…”

“…Recent studies on the serum levels of these two inflammatory cytokines in AA patients have shown contradictory results 11,14, 15 . Furthermore, recent studies on the serum levels of IL-10 in AA patients have shown paradoxical results, including both in-creasing and decreasing 16, 17 . Several studies have explored the links between AA and IL-6, IL-10 and AA and TNF-α 11,16, 18 .…”

The Role of Serum Th1, Th2, and Th17 Cytokines in Patients with Alopecia Areata: Clinical Implications

“…According to the majority of previously published studies, an increased serum level of IFN-γ was observed in patients with alopecia areata compared to healthy controls [ 22 , 23 , 25 , 26 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 ]. Higher IFN-γ expression was also detected in peripheral blood mononuclear cells in patients with alopecia areata in comparison with the control group [ 42 , 43 ].…”