Th1 polarized response induced by intramuscular DNA-HSP65 immunization is preserved in experimental atherosclerosis

Fonseca, D.M.; Bonato, Vânia Luiza Deperon; Silva, C.L.; Sartori, Alexandrina

doi:10.1590/s0100-879x2007001100010

Cited by 4 publications

(4 citation statements)

References 12 publications

(13 reference statements)

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“…In general, natural viral infections induce immune responses skewed towards the Th1 response along with cytotoxic T cells as part of antiviral immunity ( 32 33 ). To mimic the infection-like immunity as much as possible through vaccination, we adopted a DNA vaccine platform to utilize its Th1-biased systemic immune responses ( 34 35 36 ). However, the characteristics of the systemic immunity produced by natural infection with seasonal HCoVs remain incompletely understood, even in humans, likely leading to differences between respiratory infection and vaccination.…”

Section: Resultsmentioning

confidence: 99%

Pre-existing Immunity to Endemic Human Coronaviruses Does Not Affect the Immune Response to SARS-CoV-2 Spike in a Murine Vaccination Model

Jeong

Lee

et al. 2023

Immune Netw

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Endemic human coronaviruses (HCoVs) have been evidenced to be cross-reactive to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Although a correlation exists between the immunological memory to HCoVs and coronavirus disease 2019 (COVID-19) severity, there is little experimental evidence for the effects of HCoV memory on the efficacy of COVID-19 vaccines. Here, we investigated the Ag-specific immune response to COVID-19 vaccines in the presence or absence of immunological memory against HCoV spike Ags in a mouse model. Pre-existing immunity against HCoV did not affect the COVID-19 vaccine-mediated humoral response with regard to Ag-specific total IgG and neutralizing Ab levels. The specific T cell response to the COVID-19 vaccine Ag was also unaltered, regardless of pre-exposure to HCoV spike Ags. Taken together, our data suggest that COVID-19 vaccines elicit comparable immunity regardless of immunological memory to spike of endemic HCoVs in a mouse model.

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Section: Resultsmentioning

confidence: 99%

Pre-existing Immunity to Endemic Human Coronaviruses Does Not Affect the Immune Response to SARS-CoV-2 Spike in a Murine Vaccination Model

Jeong

Lee

et al. 2023

Immune Netw

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“…We tested twelve different electropulse parameter settings in this regard: a high anti-Aβ42 antibody response without inducing accompanying inflammatory cellular immune responses. We also used intradermal and not intramuscular DNA injections, as it has been described that intramuscular DNA vaccination resulted in strong cellular immune responses (39, 40). The skin is the largest organ of the human body with a total area of about 20 square feet and easily accessible for immunizations via intradermal injections.…”

Section: Discussionmentioning

confidence: 99%

Intradermal active full-length DNA Aβ42 immunization via electroporation leads to high anti-Aβ antibody levels in wild-type mice

Rosenberg

Lambracht-Washington

2018

Journal of Neuroimmunology

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Aβ immunotherapies with anti-Aβ antibody responses have high potential as possible prevention treatment for Alzheimer's disease. We have previously shown that active DNA Aβ1-42 immunization via gene gun delivery led to a non-inflammatory immune response resulting in decreased Aβ levels in brains of an immunized AD mouse model. To make DNA vaccination more applicable for clinical use, we used here intradermal electroporation. With fine tuning of the electropulse parameters, high antibody levels and low levels of inflammatory cytokines in the cellular immunoassays were observed. Full-length DNA Aβ1-42 immunization delivered via electroporation has potential to be used in the clinical setting.

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“…Mice were monitored for the onset of diabetes by measurements of blood glucose using Prestige LX Smart System test-strips (Home Diagnostic, Inc., Fort Lauderdale, FL, USA). Consecutive readings of blood glucose levels = 200 mg/dL (12 mmol/L) accompanied by glycosuria on 2 consecutive days were considered to be diagnostic of diabetes onset [ 13 ]. The incidence is expressed as percentages.…”

Section: Methodsmentioning

confidence: 99%

New strategy for testing efficacy of immunotherapeutic compounds for diabetes in vitro

et al. 2016

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BackgroundThe valuable role of immunotherapy in treating autoimmune diseases is increasingly recognized by those involved in the research and clinical application of new biopharmaceuticals products. However, many aspects related to the mechanisms of immune-modulated therapies remain to be elucidated in order to explore fully the emerging opportunities. The non-obese diabetic NOD mouse develops insulin-dependent diabetes mellitus spontaneously as a consequence of an autoimmune process in the presence of pathogenic CD4+ T cells that typically exhibit Th17 cell phenotypes. The change of a Th17 phenotype into a pattern of regulatory T cells (Treg) is extremely important in controlling autoimmune diseases. Heat shock proteins (HSPs) are stress-induced proteins with immunoregulatory properties. In the current study, the capacity of Hsp65 and Hsp70 mycobacterial HSPs and a constructed DNA encoded Hsp65 (DNAhsp65) to transform the pattern of the immune response from Th17 into Treg cells has been studied in vitro using co-cultures of antigen presenting cells (APCs) and T cells in NOD mice.ResultsCells harvested from NOD mice and cultured for 48 h (without immunoregulatory compounds) presented with Th1/Th17 patterns and secretions of IL-6, IFN-γ, IL-10 and IL-17 cytokines. The cultured cells from the non-diabetic BALB/C mice exhibited a Th1 pattern and the production of IL 6 and IFN-γ secretions. An up-regulation was observed in the supernatants from the co-cultures of NOD cells that were stimulated with DNAhsp65, Hsp65 or Hsp70 through increased levels of IL-10 secretion and the suppression of IL-6, IFN-γ and IL-17 production. In addition, immunoregulation was demonstrated through IL-17 suppression in the co-culture stimulated by the specific insulin antigen. Moreover, an increase of immunoregulatory compounds were observed in the co-culture through the expression of CD11b+CD86+ activation markers on APCs, as well as the frequency of Treg cells expressing CD4+CD3+ and CD4+CD25hi.ConclusionsThe in vitro observation of Th17 cells differentiating into Tregs in NOD mice could raise the hypothesis that the immune regulatory activity of HSPs could be an efficient strategy for diabetes prevention and treatment.

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Th1 polarized response induced by intramuscular DNA-HSP65 immunization is preserved in experimental atherosclerosis

Abstract: We previously reported that a DNA vaccine constructed with the heat shock protein (HSP65) gene from Mycobacterium leprae (DNA-

Cited by 4 publications

References 12 publications

Pre-existing Immunity to Endemic Human Coronaviruses Does Not Affect the Immune Response to SARS-CoV-2 Spike in a Murine Vaccination Model

Pre-existing Immunity to Endemic Human Coronaviruses Does Not Affect the Immune Response to SARS-CoV-2 Spike in a Murine Vaccination Model

Intradermal active full-length DNA Aβ42 immunization via electroporation leads to high anti-Aβ antibody levels in wild-type mice

New strategy for testing efficacy of immunotherapeutic compounds for diabetes in vitro

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